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1.
The G1 glycoprotein of California encephalitis (CE) virus plays a critical role in the infection of mosquito and mammalian cells. We found that CE virus enters baby hamster kidney (BHK-21) and Aedes albopictus (C6/36) cells by the endocytic pathway. Ammonium chloride, a lysosomotropic amine that prevents release of virus from endosomes, inhibited infection of both cell types when added within 10 min after viral adsorption. In addition, infected cells formed polykaryons when the extracellular pH was lowered to 6.3; optimal fusion occurred at pH 5.8 and 6.0 (C6/36 and BHK-21 cells, respectively). Two neutralizing G1 MAba, 6D5.5 and 7D4.5, inhibited low pH-induced syncytia formation without affecting viral attachment, suggesting a role for G1 in viral entry. Since viral fusion proteins have been demonstrated to undergo conformational changes at low pH, acid-induced changes in G1 and G2 were assessed. While both G1 and G2 demonstrated low pH-induced alterations in detergent binding, only G1 displayed an altered protease cleavage pattern at the fusion pH. These results indicate that the G1 protein of CE virus undergoes conformational changes necessary for low pH-mediated entry into both mosquito and mammalian cells. 相似文献
2.
PI Lobo C Spencer MT Douglas WC Stevenson TL Pruett 《Canadian Metallurgical Quarterly》1993,55(5):1063-1066
Recent reports indicate a higher incidence of both acute and chronic liver allograft rejection when, at the time of transplantation, the recipients serum contains donor-specific anti-HLA antibodies. From 9/89 to 5/91, 133 liver allografts were performed at our institution. Thirteen liver recipients had donor-specific IgG anti-HLA antibodies (complement-fixing) at the time of transplantation. In eleven patients, antibodies reacted to donor class I antigens while in 1 patient the donor-specific antibody had class II reactivity. Twelve patients have been followed for a minimum of 12 months (median 18 months, range 28-12 months). No hyperacute rejection was seen in any of the cases and four patients had acute rejections. Thus far only one of the twelve patients has biopsy evidence suggestive of chronic liver injury. The remaining have normal liver enzymes and bilirubin. Three of these twelve patients died (one from a myocardial infarction and the others from sepsis) accounting for a one-year graft survival of 75%. There was no significant statistical difference in the one-year graft survival in those recipients without donor-specific antibodies (i.e., 80.5%). In eight of the twelve patients, pretransplant preformed antibody level (PRA) was > 50%. In six of the thirteen patients donor-specific antibody was present at dilutions greater than 1:64. As previously reported, the donor-specific antibody disappeared from the serum posttransplant within hours and did not reappear. In vitro studies demonstrated no factor in portal or hepatic artery blood that could inhibit rabbit complement mediated lysis of anti-HLA antibodies. We conclude that it is not a contraindication to do liver transplants in the presence of donor-specific anti-HLA antibodies. 相似文献
3.
Saponara S. Fanucci L. Marsi S. Ramponi G. Kammler D. Witte E.M. 《Circuits and Systems II: Express Briefs, IEEE Transactions on》2007,54(7):596-600
This brief presents an application-specific instruction-set processor (ASIP) for real-time Retinex image and video filtering. Design optimizations are addressed at algorithmic and architectural levels, the latter including a dedicated memory structure, an adapted pipeline, bypasses, a custom address generator and special looping structures. Synthesized in CMOS technology, the ASIP stands for its better energy-flexibility tradeoff versus reference ASIC and digital signal processing Retinex implementations. 相似文献
4.
Lima A.M.N. Deep G.S. Neto J.S.R. Freire R.C.S. Lobo P.C. 《IEEE transactions on instrumentation and measurement》1994,43(2):133-138
An identification procedure to estimate the parameters of a thermoresistive solar radiation sensor is presented. The proposed technique employs only electrical excitation for the sensor. The estimation algorithm is recursive and is applied to the sensor model derived from the thermodynamic equilibrium differential equations. The simulation and the experimental results demonstrate the validity of the proposed approach 相似文献
5.
Digital cameras, new generation phones, commercial TV sets and, in general, all modern devices for image acquisition and visualization can benefit from algorithms for image enhancement suitable to work in real time and preferably with limited power consumption. Among the various methods described in the scientific literature, Retinex-based approaches are able to provide very good performances, but unfortunately they typically require a high computational effort. In this article, we propose a flexible and effective architecture for the real-time enhancement of video frames, suitable to be implemented in a single FPGA device. The video enhancement algorithm is based on a modified version of the Retinex approach. This method, developed to control the dynamic range of poorly illuminated images while preserving the visual details, has been improved by the adoption of a new model to perform illuminance estimation. The video enhancement parameters are controlled in real time through an embedded microprocessor which makes the system able to modify its behavior according to the characteristics of the input images, and using information about the surrounding light conditions. 相似文献
6.
A.c. impedance behaviour of MnO2 and doped -MnO2 electrodes in H2SO4 medium was assessed with a view to explaining the mechanism of the discharge behaviour of MnO2 electrodes in 4m H2SO4 electrolyte. The electrodes used in this work appear to be intermediate cases of planar and porous electrodes as the angles, , made by the low frequency part with the real axis are found to be in the range (30–60°). The Nyquist plots and the Randle plots tend to reinforce the observation made by Tye that the capacity yield is essentially diffusion controlled. The depression and flattening of semi-circles observed reveals a link with the heterogeneity of the planar electrode and with the porosity of the pitted electrode. The deviation from a 45° angle made by the low frequency part with the real axis may either be explained by the roughness of the electrode surface or the shallow pores on the surface of the electrodes; in other words due to the difference between the apparent and true surface areas. The double layer capacitance values of the electrodes seem to subsume adsorption capacitances and diffusion factors. Hence, the relative increase in magnitude. The electrodes appear to behave like planar electrodes when 10 µF is introduced into the circuit as a parallel capacitance since angles 0 vary between 40–58°. The undoped -MnO2 electrode, as well as those prepared from Li-MnO2, Ag-MnO2, and I.C.8, appear to be planar electrodes. 相似文献
7.
MR Harrison GB Mychaliska CT Albanese RW Jennings JA Farrell S Hawgood P Sandberg AH Levine E Lobo RA Filly 《Canadian Metallurgical Quarterly》1998,33(7):1017-22; discussion 1022-3
BACKGROUND/PURPOSE: Fetuses with congenital diaphragmatic hernia (CDH) who have a "poor prognosis" with postnatal treatment now can be identified on the basis of liver herniation, early diagnosis (before 25 weeks' gestation) and a low lung-to-head ratio (LHR). Because complete in utero repair proved unsuccessful for this group, the strategy of temporary tracheal occlusion was developed to gradually enlarge the hypoplastic fetal lung. The purpose of this study is to compare the outcome of patients in the poor-prognosis group treated by one of three methods: (1) standard postnatal care, (2) fetal tracheal occlusion via open hysterotomy, and (3) the recently developed video-fetoscopic (Fetendo) technique of tracheal occlusion without hysterotomy. METHODS: In the past 3 years, 34 of 86 fetuses with an isolated left CDH met criteria for the poor-prognosis group. Thirteen families chose postnatal treatment at an extracorporeal membrane oxygenation (ECMO) center, 13 underwent open fetal tracheal occlusion, and eight underwent fetoscopic tracheal occlusion. RESULTS: The survival rate was 38% in the group treated by standard postnatal therapy, 15% in the open tracheal occlusion group, and 75% in the Fetendo group. There were less postoperative pulmonary complications noted in mothers who underwent the Fetendo procedure versus the open tracheal occlusion. All but one Fetendo clip patient had a striking physiological response demonstrated by sonographic enlargement of the small left lung that was documented postnatally by plain radiographs and its subjective appearance during repair of the CDH. In contrast, only 5 of the 13 open tracheal occlusion patients demonstrated lung growth. CONCLUSION: Fetuses with a left CDH who have liver herniation and a low LHR are at high risk of neonatal demise and appear to benefit from temporary tracheal occlusion when performed fetoscopically, but not when performed by open fetal surgery. 相似文献
8.
Dan Lin Prathima Rao Elisa Bertino Ninghui Li Jorge Lobo 《International Journal of Information Security》2010,9(4):253-273
Policy integration and inter-operation is often a crucial requirement when parties with different access control policies
need to participate in collaborative applications and coalitions. Such requirement is even more difficult to address for dynamic
large-scale collaborations, in which the number of access control policies to analyze and compare can be quite large. An important
step in policy integration and inter-operation is to analyze the similarity of policies. Policy similarity can sometimes also
be a pre-condition for establishing a collaboration, in that a party may enter a collaboration with another party only if
the policies enforced by the other party match or are very close to its own policies. Existing approaches to the problem of
analyzing and comparing access control policies are very limited, in that they only deal with some special cases. By recognizing
that a suitable approach to the policy analysis and comparison requires combining different approaches, we propose in this
paper a comprehensive environment—EXAM. The environment supports various types of analysis query, which we categorize in the
paper. A key component of such environment, on which we focus in the paper, is the policy analyzer able to perform several
types of analysis. Specifically, our policy analyzer combines the advantages of existing MTBDD-based and SAT-solver-based
techniques. Our experimental results, also reported in the paper, demonstrate the efficiency of our analyzer. 相似文献
9.
Algorithmic and architectural design for real-time and power-efficient Retinex image/video processing 总被引:1,自引:1,他引:0
Sergio Saponara Luca Fanucci Stefano Marsi Giovanni Ramponi 《Journal of Real-Time Image Processing》2007,1(4):267-283
This paper presents novel algorithmic and architectural solutions for real-time and power-efficient enhancement of images
and video sequences. A programmable class of Retinex-like filters, based on the separation of the illumination and reflectance
components, is proposed. The dynamic range of the input image is controlled by applying a suitable non-linear function to
the illumination, while the details are enhanced by processing the reflectance. An innovative spatially recursive rational
filter is used to estimate the illumination. Moreover, to improve the visual quality results of two-branch Retinex operators
when applied to videos, a novel three-branch technique is proposed which exploits both spatial and temporal filtering. Real-time
implementation is obtained by designing an Application Specific Instruction-set Processor (ASIP). Optimizations are addressed
at algorithmic and architectural levels. The former involves arithmetic accuracy definition and linearization of non-linear
operators; the latter includes customized instruction set, dedicated memory structure, adapted pipeline, bypasses, custom
address generator, and special looping structures. The ASIP is synthesized in standard-cells CMOS technology and its performances
are compared to known Digital signal processor (DSP) implementations of real-time Retinex filters. As a result of the comparison,
the proposed algorithmic/architectural design outperforms state-of-art Retinex-like operators achieving the best trade-off
between power consumption, flexibility, and visual quality.
Sergio Saponara is a Research Scientist and Assistant Professor at the University of Pisa. He was born in Bari, Italy, in 1975. He received the Electronic Engineering degree cum laude and the Ph.D. in Information Engineering, both from Pisa University, in 1999 and 2003, respectively. Since 2001 he collaborates with Consorzio Pisa Ricerche, Italy and in 2002 he was with IMEC, Belgium as Marie Curie research fellow. His research and teaching interests include electronic circuits and systems for multimedia, telecom and automation. He co-authored more than 40 papers including journals, conferences and patents. Luca Fanucci is Associate Professor of Microelectronics at the University of Pisa. He was born in Montecatini, Italy, in 1965. He received the Doctor Engineer degree and the Ph.D. in Electronic Engineering from the University of Pisa in 1992 and 1996, respectively. From 1992 to 1996, he was with the European Space Agency's Research and Technology Center, Noordwijk, The Netherlands, and from 1996 to 2004 he was a Research Scientist of the Italian National Research Council in Pisa. His research interests include design technologies for integrated circuits and systems, with emphasis on system-level design, hardware/software co-design and low-power. He co-authored more than 100 journal/conference papers and holds more than 10 patents. Stefano Marsi was born in Trieste, Italy, in 1963. He received the Doctor Engineer degree in Electronic Engineering (summa cum laude) in 1990 and the Ph.D. degree in 1994. Since 1995 he has held the position of researcher in the Department of Electronics at the University of Trieste where he is the teacher of courses in electronic field. His research interests include non-linear operators for image and video processing and their realization through application specific electronics circuits. He is author or co-author of more than 40 papers in international journals, proceedings of international conferences or contributions in books. Giovanni Ramponi is Professor of Electronics at the Department of Electronics of the University of Trieste, Italy. His research interests include nonlinear digital signal processing, and the enhancement and feature extraction in images and image sequences. Prof. Ramponi has been an Associate Editor of the IEEE Signal Processing Letters and of the IEEE Transactions on Image Processing; presently is an AE of the SPIE Journal of Electronic Imaging. He has participated in various EU and National Research Projects. He is the co-inventor of various pending international patents and has published more than 140 papers in international journals and conference proceedings, and as book chapters. Prof. Ramponi contributes to several undergraduate and graduate courses on digital signal processing. 相似文献
Giovanni RamponiEmail: |
Sergio Saponara is a Research Scientist and Assistant Professor at the University of Pisa. He was born in Bari, Italy, in 1975. He received the Electronic Engineering degree cum laude and the Ph.D. in Information Engineering, both from Pisa University, in 1999 and 2003, respectively. Since 2001 he collaborates with Consorzio Pisa Ricerche, Italy and in 2002 he was with IMEC, Belgium as Marie Curie research fellow. His research and teaching interests include electronic circuits and systems for multimedia, telecom and automation. He co-authored more than 40 papers including journals, conferences and patents. Luca Fanucci is Associate Professor of Microelectronics at the University of Pisa. He was born in Montecatini, Italy, in 1965. He received the Doctor Engineer degree and the Ph.D. in Electronic Engineering from the University of Pisa in 1992 and 1996, respectively. From 1992 to 1996, he was with the European Space Agency's Research and Technology Center, Noordwijk, The Netherlands, and from 1996 to 2004 he was a Research Scientist of the Italian National Research Council in Pisa. His research interests include design technologies for integrated circuits and systems, with emphasis on system-level design, hardware/software co-design and low-power. He co-authored more than 100 journal/conference papers and holds more than 10 patents. Stefano Marsi was born in Trieste, Italy, in 1963. He received the Doctor Engineer degree in Electronic Engineering (summa cum laude) in 1990 and the Ph.D. degree in 1994. Since 1995 he has held the position of researcher in the Department of Electronics at the University of Trieste where he is the teacher of courses in electronic field. His research interests include non-linear operators for image and video processing and their realization through application specific electronics circuits. He is author or co-author of more than 40 papers in international journals, proceedings of international conferences or contributions in books. Giovanni Ramponi is Professor of Electronics at the Department of Electronics of the University of Trieste, Italy. His research interests include nonlinear digital signal processing, and the enhancement and feature extraction in images and image sequences. Prof. Ramponi has been an Associate Editor of the IEEE Signal Processing Letters and of the IEEE Transactions on Image Processing; presently is an AE of the SPIE Journal of Electronic Imaging. He has participated in various EU and National Research Projects. He is the co-inventor of various pending international patents and has published more than 140 papers in international journals and conference proceedings, and as book chapters. Prof. Ramponi contributes to several undergraduate and graduate courses on digital signal processing. 相似文献
10.
The ??systematicity argument?? has been used to argue for a classical cognitive architecture (Fodor in The Language of Thought. Harvester Press, London, 1975, Why there still has to be a language of thought? In Psychosemantics, appendix. MIT Press, Cambridge, pp 135?C154, 1987; Fodor and Pylyshyn in Cognition 28:3?C71, 1988; Aizawa in The systematicity arguments. Kluwer Academic Press, Dordrecht, 2003). From the premises that cognition is systematic and that the best/only explanation of systematicity is compositional structure, it concludes that cognition is to be explained in terms of symbols (in a language of thought) and formal rules. The debate, with connectionism, has mostly centered on the second premise-whether an explanation of systematicity requires compositional structure, which neural networks do not to exhibit (for example, Hadley and Hayward, in Minds and Machines, 7:1?C37). In this paper, I will take issue with the first premise. Several arguments will be deployed that show that cognition is not systematic in general; that, in fact, systematicity seems to be related to language. I will argue that it is just verbal minds that are systematic, and they are so because of the structuring role of language in cognition. A dual-process theory of cognition will be defended as the best explanation of the facts. 相似文献