全文获取类型
收费全文 | 305篇 |
免费 | 17篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 1篇 |
化学工业 | 164篇 |
金属工艺 | 7篇 |
机械仪表 | 7篇 |
建筑科学 | 7篇 |
能源动力 | 11篇 |
轻工业 | 34篇 |
无线电 | 3篇 |
一般工业技术 | 45篇 |
冶金工业 | 32篇 |
原子能技术 | 3篇 |
自动化技术 | 9篇 |
出版年
2024年 | 1篇 |
2023年 | 5篇 |
2022年 | 34篇 |
2021年 | 59篇 |
2020年 | 14篇 |
2019年 | 11篇 |
2018年 | 6篇 |
2017年 | 3篇 |
2016年 | 18篇 |
2015年 | 5篇 |
2014年 | 19篇 |
2013年 | 8篇 |
2012年 | 12篇 |
2011年 | 17篇 |
2010年 | 7篇 |
2009年 | 9篇 |
2008年 | 14篇 |
2007年 | 15篇 |
2006年 | 6篇 |
2005年 | 6篇 |
2004年 | 4篇 |
2003年 | 2篇 |
2002年 | 7篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1998年 | 6篇 |
1997年 | 4篇 |
1996年 | 4篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1991年 | 4篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有323条查询结果,搜索用时 15 毫秒
1.
2.
Marta Krychowiak-Manicka Mirosawa Krauze-Baranowska Sylwia Godlewska Zbigniew Kaczyski Aleksandra Bielicka-Giedo Natalia Grzegorczyk Magdalena Narajczyk Joanna E. Frackowiak Aleksandra Krolicka 《International journal of molecular sciences》2021,22(9)
Carnivorous plants are exemplary natural sources of secondary metabolites with biological activity. However, the therapeutic antimicrobial potential of these compounds is limited due to intrinsic resistance of selected bacterial pathogens, among which Pseudomonas aeruginosa represents an extreme example. The objective of the study was to overcome the intrinsic resistance of P. aeruginosa by combining silver nanoparticles (AgNPs) with secondary metabolites from selected carnivorous plant species. We employed the broth microdilution method, the checkerboard titration technique and comprehensive phytochemical analyses to define interactions between nanoparticles and active compounds from carnivorous plants. It has been confirmed that P. aeruginosa is resistant to a broad range of secondary metabolites from carnivorous plants, i.e., naphthoquinones, flavonoids, phenolic acids (MBC = 512 µg mL−1) and only weakly sensitive to their mixtures, i.e., extracts and extracts’ fractions. However, it was shown that the antimicrobial activity of extracts and fractions with a significant level of naphthoquinone (plumbagin) was significantly enhanced by AgNPs. Our studies clearly demonstrated a crucial role of naphthoquinones in AgNPs and extract interaction, as well as depicted the potential of AgNPs to restore the bactericidal activity of naphthoquinones towards P. aeruginosa. Our findings indicate the significant potential of nanoparticles to modulate the activity of selected secondary metabolites and revisit their antimicrobial potential towards human pathogenic bacteria. 相似文献
3.
Miniaturizing experimental sample volumes to the nanoliter volume range is one of the most economical ways to perform mid- and high-throughput compound screening experiments. Existing automation platforms for nanoliter fluid handling can be bulky, expensive, and require periodic calibration to provide consistent liquid dispensing. In addition, even with frequent calibration, significant instrument-to-instrument variation in low-volume dispensing can occur between different instrument platforms. Many of these issues can be addressed by the use of PocketTips. PocketTips are tips with a defined internal pocket designed to hold specific nanoliter volumes of compound dissolved in dimethylsulfoxide. Although the overall liquid-handling process with PocketTips uses the aspirate/dispense features of the specific liquid-handling device being used, the dispensed nanoliter volume is solely based on the dimensions of the pocket of the PocketTip and thus, the liquid-handling device itself need not have nanoliter dispensing capabilities. In this report, we demonstrate the performance of PocketTips on different automation platforms. In addition, we used a cell-based ?-lactamase reporter assay system to demonstrate that compound delivery by PocketTips compares favorably with a standard compound addition technique. 相似文献
4.
Gene expression changes in a transgenic mouse model overexpressing human wildtype and mutant torsinA
Grundmann K Hübener J Häbig K Reischmann B Poths S Hauser TK Magg J Riess O Bonin M Nguyen HP 《Proteomics. Clinical applications》2008,2(5):720-736
Primary torsion dystonia is an autosomal-dominantly inherited, neurodevelopmental movement disorder caused by a GAG deletion (ΔGAG) in the DYT1 gene, encoding torsinA. This mutation is responsible for approximately 70% of cases of early-onset primary torsion dystonia. The function of wildtype torsinA is still unknown, and it is unsolved how the deletion in the DYT1 gene contributes to the development of the disease. To better understand the molecular processes involved in torsinA pathology, we used genome-wide oligonucleotide microarrays to characterize gene expression patterns in the striatum of mouse models overexpressing the human wildtype and mutant torsinA. By this approach we were able to detect gene expression changes that seem to be specific for torsinA pathology. We found an impact of torsinA, independent from genotype, on vesicle trafficking, exocytosis, and neurotransmitter release in our mouse model. In addition, we were able to identify several new pathways and processes involved in the development of the nervous system that are affected by wildtype and mutant torsinA. Furthermore, we have striking evidence from our gene expression data that glutamate receptor mediated synaptic plasticity in the striatum is the affected underlying cellular process for impaired motor learning in human ΔGAG torsinA transgenic mice. 相似文献
5.
Andrzej Taube Robert Mroczyński Katarzyna Korwin-Mikke Sylwia Gierałtowska Jan Szmidt Anna Piotrowska 《Materials Science and Engineering: B》2012,177(15):1281-1285
In this work, we report on effects of post-deposition annealing on electrical characteristics of metal–insulator–semiconductor (MIS) structures with HfO2/SiO2 double gate dielectric stacks. Obtained results have shown the deterioration of electro-physical properties of MIS structures, e.g. higher interface traps density in the middle of silicon forbidden band (Ditmb), as well as non-uniform distribution and decrease of breakdown voltage (Ubr) values, after annealing above 400 °C. Two potential hypothesis of such behavior were proposed: the formation of interfacial layer between hafnia and silicon dioxide and the increase of crystallinity of HfO2 due to the high temperature treatment. Furthermore, the analysis of conduction mechanisms in investigated stacks revealed Poole–Frenkel (P–F) tunneling at broad range of electric field intensity. 相似文献
6.
7.
8.
Fatou Ndoye Muhammad Sulaiman Yousafzai Giovanna Coceano Serena Bonin Giacinto Scoles Oumar Ka 《International Journal of Optomechatronics》2016,10(1):53-62
We studied the lateral forces arising during the vertical indentation of the cell membrane by an optically trapped microbead, using back focal plane interferometry to determine force components in all directions. We analyzed the cell-microbead interaction and showed that indeed the force had also lateral components. Using the Hertz model, we calculated and compared the elastic moduli resulting from the total and vertical forces, showing that the differences are important and the total force should be considered. To confirm our results we analyzed cells from two breast cancer cell lines: MDA-MB-231 and HBL-100, known to have different cancer aggressiveness and hence stiffness. 相似文献
9.
Sour cherry (Prunus cerasus) is rich in biologically active phenolic compounds. These compounds are concentrated in fruit skin and most of them remain in the leftovers during the production of juice. Supercritical carbon dioxide extraction was used to separate phenolic compounds from sour cherry pomace. The effects of temperature, pressure and the addition of ethanol on anthocyanin and the total phenolic content and radical scavenging activity of the extracts were investigated. The best results were acquired for 35°C, 10 MPa and 80% ethanol addition. A strong correlation was found between the phenolic content and other features of the extracts. 相似文献
10.
Determination of Iron Species in Samples of Iron-Fortified Food 总被引:1,自引:0,他引:1
Przemyslaw Niedzielski Magdalena Zielinska-Dawidziak Lidia Kozak Pawel Kowalewski Barbara Szlachetka Sylwia Zalicka Weronika Wachowiak 《Food Analytical Methods》2014,7(10):2023-2032
The paper presents the determination of iron forms in food products. The procedure of sample extraction was developed and optimized, preserving the content of particular forms of iron. The colorimetric method using 2,2′-bipirydyl (measurements at 520 nm) was applied in Fe(II) determinations, while in Fe(III) determinations, the colorimetric method with potassium thiocyanate (measurements at 470 nm) was applied. The total content of iron was determined by the technique of atomic absorption spectrometry, which allowed for the determination of iron content in organic and inorganic complex compounds. Detection limits of 1 mg kg?1 were obtained for all determined iron forms, with the precision ranging between 0.7 % and 1.5 % for 10 mg kg?1 concentration. The optimized analytical procedure was applied in the determinations of iron forms in iron-fortified food products. 相似文献