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排序方式: 共有160条查询结果,搜索用时 15 毫秒
1.
H Asahara K Fujisawa T Kobata T Hasunuma T Maeda M Asanuma N Ogawa H Inoue T Sumida K Nishioka 《Canadian Metallurgical Quarterly》1997,40(5):912-918
Green fluorescent protein (GFP) is increasingly being used in plant biology from the cellular level to whole plant level. At the cellular level, GFP is being used as an in vivo reporter to assess frequency of transient and stable transformation. GFP has also proven to be an invaluable tool in monitoring trafficking and subcellular localization of protein. At the organ level and up, many exciting applications are rapidly emerging. The development of brighter GFP mutants with more robust folding properties has enabled better macroscopic visualization of GFP in whole leaves and plants. One interesting example has been the use of GFP to monitor virus movement in and among whole plants. GFP is also emerging as a powerful tool to monitor transgene movement and transgenic plants in the field. In a proof-of-concept study, tobacco was transformed with a modified version of the GFP gene controlled by a constitutive (35S) promoter. GFP expression in progeny plants ranged from 0% to 0.5%, and approximately 0.1% GFP was the minimal amount needed for unambiguous macroscopic detection. GFP is the first truly in vivo reporter system useful in whole plants, and we project its usefulness will increase even further as better forms of GFP genes become available. 相似文献
2.
Keiichi Tomishige Tomohisa Miyazawa Takeo Kimura Kimio Kunimori Naoto Koizumi Muneyoshi Yamada 《Applied catalysis. B, Environmental》2005,60(3-4):299-307
In the partial oxidation of tar derived from the pyrolysis of cedar wood, the effect of H2S addition was investigated over non-catalyst, steam reforming Ni catalyst, and Rh/CeO2/SiO2 using a fluidized bed reactor. In the non-catalytic gasification, the product distribution was not influenced by the presence of H2S. Steam reforming Ni catalyst was effective for the tar removal without H2S addition, however, the addition of H2S deactivated drastically. In contrast, Rh/CeO2/SiO2 exhibited higher and more stable activity than the Ni catalyst even under the presence of high concentration of H2S (280 ppm). On the Ni catalyst, the adsorption of sulfur was observed by XPS and Ni species was oxidized during the partial oxidation of tar. In the case of Rh/CeO2/SiO2, the adsorption of sulfur was below the detection limit of XPS. This can be related to the self-cleaning of catalyst surface during the circulation in the fluidized bed reactor for the partial oxidation of tar derived from cedar pyrolysis. 相似文献
3.
Methylcyclohexane dehydrogenation for hydrogen production via a bimodal catalytic membrane reactor 下载免费PDF全文
Lie Meng Xin Yu Takuya Niimi Hiroki Nagasawa Masakoto Kanezashi Tomohisa Yoshioka Toshinori Tsuru 《American Institute of Chemical Engineers》2015,61(5):1628-1638
The dehydrogenation of methylcyclohexane (MCH) to toluene (TOL) for hydrogen production was theoretically and experimentally investigated in a bimodal catalytic membrane reactor (CMR), that combined Pt/Al2O3 catalysts with a hydrogen‐selective organosilica membrane prepared via sol‐gel processing using bis(triethoxysilyl) ethane (BTESE). Effects of operating conditions on the membrane reactor performance were systematically investigated, and the experimental results were in good agreement with those calculated by a simulation model with a fitted catalyst loading. With H2 extraction from the reaction stream to the permeate stream, MCH conversion at 250°C was significantly increased beyond the equilibrium conversion of 0.44–0.86. Because of the high H2 selectivity and permeance of BTESE‐derived membranes, a H2 flow with purity higher than 99.8% was obtained in the permeate stream, and the H2 recovery ratio reached 0.99 in a pressurized reactor. A system that combined the CMR with a fixed‐bed prereactor was proposed for MCH dehydrogenation. © 2015 American Institute of Chemical Engineers AIChE J, 61: 1628–1638, 2015 相似文献
4.
Biosynthesis of Trehangelin in Polymorphospora rubra K07–0510: Identification of Metabolic Pathway to Angelyl‐CoA 下载免费PDF全文
Dr. Yuki Inahashi Taro Shiraishi Dr. Kaia Palm Prof. Dr. Yoko Takahashi Prof. Dr. Satoshi Ōmura Prof. Dr. Tomohisa Kuzuyama Dr. Takuji Nakashima 《Chembiochem : a European journal of chemical biology》2016,17(15):1442-1447
Trehangelins are trehalose angelates discovered from endophytic actinomycete Polymorphospora rubra K07‐0510. We identified the trehangelin biosynthetic gene cluster, including genes that encode a glycoside hydrolase‐like protein (thgC), α‐amylase (thgD), 3‐ketoacyl‐ACP synthase III (thgI), 3‐ketoacyl‐ACP reductase (thgK), enoyl‐CoA hydratase (thgH) and acyl transferase (thgJ). Heterologous expression of thgH, thgI, thgJ and thgK confirmed the importance of these genes in the biosynthesis of trehangelin A. Enzymatic activity studies showed that ThgI catalyses the condensation of acetyl‐CoA and methylmalonyl‐CoA to 2‐methylacetoacetyl‐CoA (MAA‐CoA), ThgK catalyses NADPH‐dependent reduction of MAA‐CoA to 3‐hydroxy‐2‐methylbutyryl‐CoA (HMB‐CoA) and ThgH catalyses the dehydration of HMB‐CoA to angelyl‐CoA (AN‐CoA). This is the first report on the elucidation of the enzymatic formation of AN‐CoA. 相似文献
5.
A. Takeoka Y. Fukuda M. Suzuki M. Hasunuma E. Sakoguchi H. Tokizaki S. Kouzuma M. Waki M. Ohnishi S. Nakano Y. Kuwano 《Progress in Photovoltaics: Research and Applications》1993,1(1):47-54
We have successfully developed and industrialized a solar-powered air conditioner that operates through a combination of photovoltaic (PV) arrays and commercial power supplies. In order to achieve the most efficient operation possible from a one-horsepower inverter-type household air conditioner, we selected a PV array output of 540 W under standard test conditions, based on single-day power use patterns for air conditioners as well as on solar cell output patterns. When using a 540-W PV array, we have shown that the system can supply at least 90% of the power consumed by an air conditioner during peak power periods in summer. We have confirmed that on average the power generated by the PV array can satisfy at least 50% of the power consumed by an air conditioner in summer. We also confirmed that the system functions according to system design specifications. There is little doubt that the introduction and widespread use of this system will play an important role in reducing peak power energy requirements in summer. 相似文献
6.
A photocatalytic membrane reactor for gas-phase reactions using porous titanium oxide membranes 总被引:10,自引:0,他引:10
Toshinori Tsuru Takehiro Kan-no Tomohisa Yoshioka Masashi Asaeda 《Catalysis Today》2003,82(1-4):41-48
Photocatalytic membrane reactors using porous titanium oxide membranes having pore sizes of several nanometers were utilized for a gas-phase reaction of methanol. Air mixed with methanol (MeOH) vapor, the concentration of which was controlled in the range of 500–6000 ppm, was fed to the photocatalytic membrane reactor in the range of 50–500 cm3/min using several types of flow patterns. Photocatalysis with membrane permeation resulted in a large decomposition rate, compared to photocatalysis without membrane permeation. The characteristics of the reaction such as decomposition ratio of MeOH, the conversion of the decomposed MeOH to CO2 and H2O were found to be a function of the residence time in the reactor. The photocatalytic reaction was analyzed based on pseudo-first-order kinetics to ascertain its simplicity, and the fitted curves were found to be in a relatively good agreement with the experimental data. Apparent rate constants with and without membrane permeation were 2.5 and 1.5×10−6 m s−1, respectively, indicating that the performance of the photocatalytic reaction system with membrane permeation was enhanced. 相似文献
7.
Rong Xu Jinhui Wang Masakoto Kanezashi Tomohisa Yoshioka Toshinori Tsuru 《American Institute of Chemical Engineers》2013,59(4):1298-1307
Hybrid organosilica membranes were successfully prepared using bis(triethoxysilyl)ethane (BTESE) and applied to reverse osmosis (RO) desalination. The organosilica membrane calcined at 300°C almost completely rejected salts and neutral solutes with low‐molecular‐weight. Increasing the operating pressure led to an increase in water flux and salt rejection, while the flux and rejection decreased as salt concentration increased. The water permeation mechanism differed from the viscous flow mechanism. Observed activation energies for permeation were larger for membranes with a smaller pore size, and were considerably larger than the activation energy for water viscosity. The organosilica membranes exhibited exceptional hydrothermal stability in temperature cycles up to 90°C. The applicability of the generalized solution‐diffusion (SD) model to RO and pervaporation (PV) desalination processes were examined, and the quantitative differences in water permeance were accurately predicted by the application of generalized transport equations. © 2012 American Institute of Chemical Engineers AIChE J, 59: 1298–1307, 2013 相似文献
8.
Shiomi N Yasuda T Inoue Y Kusumoto N Iwasaki S Katsuda T Katoh S 《Journal of Bioscience and Bioengineering》2004,97(1):54-58
Soil microorganisms play an important role in maintaining soil pH at levels suitable for other soil organisms. To clarify the biological neutralization mechanism in soil, we isolated soil microorganisms showing a high ability to neutralize acids and studied their characteristics. From our taxonomic study, three isolated strains were identified as filamentous fungi, namely Mucor sp., Aspergillus fumigatus, and Aureobasidium pullulans. These strains could secrete basic materials, such as ammonia, for neutralization, grow in the medium at pH 4.0 and increase the pH of the medium to approximately 8.0. These microbial cells could neutralize not only nitric acid but also sulfuric and hydrochloric acids. The strains could also grow by utilizing nitric acid as a sole nitrogen source. In the soil containing these organisms, the pH was maintained in the neutral range by the buffering action of basic materials that they secrete. These results suggest that these fungal cells are useful for protecting the soil from acidification by acid rain. 相似文献
9.
Yamaguchi A Uejo F Yoda T Uchida T Tanamura Y Yamashita T Teramae N 《Nature materials》2004,3(5):337-341
A mesoporous membrane composed of nanochannels with a uniform diameter has a potential use for precise size-exclusive separation of molecules. Here, we report a novel method to form a hybrid membrane composed of silica-surfactant nanocomposite and a porous alumina membrane, by which size-selective transport of molecules across the membrane becomes possible. The nanocomposite formed inside each columnar alumina pore was an assembly of surfactant-templated silica-nanochannels with a channel diameter of 3.4 nm; the channel direction being predominantly oriented along the wall of the columnar alumina pore. Molecules could be transported across the membrane including the silica-surfactant nanocomposite with a capability of nanometre-order size-exclusive separation. Our proposed membrane system has a potential use not only for separation science, but also catalysis and chip technologies. 相似文献
10.
A classification technique using the neural networks has recently been developed. We apply a neural network of learning vector
quantization (LVQ) to classify remote-sensing data, including microwave and optical sensors, for the estimation of a rice-planted
area. The method has the capability of nonlinear discrimination, and the classification function is determined by learning.
The satellite data were observed before and after planting rice in 1999. Three sets of RADARSAT and one set of SPOT/HRV data
were used in Higashi–Hiroshima, Japan. Three RADARSAT images from April to June were used for this study. The LVQ classification
was applied the RADARSAT and SPOT to evaluate the estimate of the area of planted-rice. The results show that the true production
rate of the rice-planted area estimation of RADASAT by LVQ was approximately 60% compared with that of SPOT by LVQ. It is
shown that the present method is much better than the SAR image classification by the maximum likelihood method. 相似文献