Collective Practices in Common Information Spaces: Insight From Two Case Studies

This research explores the design of practice toolkits as components, distinct from community management systems, allowing members of a virtual community to engage in the practice the community is about. Our analysis is informed by two case studies in different application domains each presenting alternative but complementary insights to the design of computer-mediated practice toolkits. The first case study describes how established practices in music performance are encapsulated in a suitably augmented music notation toolkit so as to support the learning objectives of virtual teams engaged in music master classes. The second case study presents experience with the development of a toolkit for engaging in the practice of vacation package assembly. This time the virtual team is a cross-organization virtual community of practice whose members streamline their efforts by internalizing and performing in accordance to a new (virtual) practice. Findings from the two studies reveal two distinct orientations in the design of practice toolkits. Specifically, in application domains where practices are well established (i.e., music performance), the toolkit serves as the medium for reconstructing an existing practice in virtual settings. In contrast, when cross-organization collaboration is involved (i.e., vacation package assembly), the toolkit should be designed so as to encapsulate a “meta”-practice, exhibiting both boundary and locality.     

Determination of triterpenic acids in natural and alkaline-treated Greek table olives throughout the fermentation process

Maslinic and oleanolic acids are among the most abundant triterpenic acids found in olive fruits. Both acids are considered to be important bioactive compounds which can confer multiple beneficial health effects to the consumer. In the present work, we have monitored and quantified maslinic and oleanolic acids throughout processing in alkaline-treated green olives (Spanish-style) and in natural green olives of the Conservolea variety that is particularly popular in Greece. Our findings clearly demonstrate that the fast de-bittering process with NaOH treatment in Spanish-style olives has a profound negative effect on the concentration of both acids. This decrease of concentration was more prominent regarding maslinic acid when compared to oleanolic acid. In contrast, the slow de-bittering during natural fermentation of green olives had no effect on the content of maslinic or oleanolic acid. To verify the broad applicability of our observation we have also looked into the natural processing of the Kalamon variety (Greek-style). Our findings were consistent, since once again, natural fermentation did not influence the concentration of both acids.     

Decitabine Treatment Induces a Viral Mimicry Response in Cervical Cancer Cells and Further Sensitizes Cells to Chemotherapy

Purpose: To investigate the anti-cancer, chemosensitizing and/or immunomodulating effects of decitabine (DAC) to be used as a potential therapeutic agent for the treatment of cervical cancer (CC). Methods: Cervical cancer cell lines were treated with low doses of DAC treatment used as a single agent or in combination with chemotherapy. End-point in vitro assays were developed as indicators of the anti-cancer and/or immunomodulating effects of DAC treatment in CC cells. These assays include cell viability, cell cycle analysis, apoptosis, induction of a viral-mimicry response pathway, expression of MHC-class I and PD-L1 and chemosensitivity. Results: High and low doses of DAC treatment induced reduction in cell viability in HeLa (HPV18+), CaSki (HPV16+) and C33A (HPV−) cells. Specifically, a time-dependent reduction in cell viability of HeLa and CaSki cells was observed accompanied by robust cell cycle arrest at G2/M phase and alterations in the cell cycle distribution. Decrease in cell viability was also observed in a non-transformed immortal keratinocyte (HaCat) suggesting a non-cancer specific target effect. DAC treatment also triggered a viral mimicry response through long-term induction of cytoplasmic double-stranded RNA (dsRNA) and activation of downstream IFN-related genes in both HPV+ and HPV− cells. In addition, DAC treatment increased the number of CC cells expressing MHC-class I and PD-L1. Furthermore, DAC significantly increased the proportion of early and late apoptotic CC cells quantified using FACS. Our combination treatments showed that low dose DAC treatment sensitizes cells to chemotherapy. Conclusions: Low doses of DAC treatment promotes robust induction of a viral mimicry response, immunomodulating and chemosensitizing effects in CC, indicating its promising therapeutic role in CC in vitro.     

Sequence divergence of the RNA polymerase shared subunit ABC14.5 (Rpb8) selectively affects RNA polymerase III assembly in Saccharomyces cerevisiae

ABC14.5 (Rpb8) is a eukaryotic subunit common to all three nuclear RNA polymerases. In Saccharomyces cerevisiae, ABC14.5 (Rpb8) is essential for cell viability, however its function remains unknown. We have cloned and characterised the Schizosaccharomyces pombe rpb8(+) cDNA. We found that S.pombe rpb8, unlike the similarly diverged human orthologue, cannot substitute for S.cerevisiae ABC14. 5 in vivo. To obtain information on the function of this RNA polymerase shared subunit we have used S.pombe rpb8 as a naturally altered molecule in heterologous expression assays in S.cerevisiae. Amino acid residue differences within the 67 N-terminal residues contribute to the functional distinction of the two yeast orthologues in S.cerevisiae. Overexpression of the S.cerevisiae largest subunit of RNA polymerase III C160 (Rpc1) allows S.pombe rpb8 to functionally replace ABC14.5 in S.cerevisiae, suggesting a specific genetic interaction between the S.cerevisiae ABC14.5 (Rpb8) and C160 subunits. We provide further molecular and biochemical evidence showing that the heterologously expressed S.pombe rpb8 molecule selectively affects RNApolymerase III but not RNA polymerase I complex assembly. We also report the identification of a S.cerevisiae ABC14.5-G120D mutant which affects RNA polymerase III.     

Fuzzy cognitive map architectures for medical decision support systems

Medical decision support systems can provide assistance in crucial clinical judgments, particularly for inexperienced medical professionals. Fuzzy cognitive maps (FCMs) is a soft computing technique for modeling complex systems, which follows an approach similar to human reasoning and the human decision-making process. FCMs can successfully represent knowledge and human experience, introducing concepts to represent the essential elements and the cause and effect relationships among the concepts to model the behavior of any system. Medical decision systems are complex systems that can be decomposed to non-related and related subsystems and elements, where many factors have to be taken into consideration that may be complementary, contradictory, and competitive; these factors influence each other and determine the overall clinical decision with a different degree. Thus, FCMs are suitable for medical decision support systems and appropriate FCM architectures are proposed and developed as well as the corresponding examples from two medical disciplines, i.e. speech and language pathology and obstetrics, are described.     

XI. Yeast sequencing reports. The complete sequencing of a 24·6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases,membrane transporters,hydantoinases and the phospholipase A2-activating protein

We report the entire sequence of a 26·4 kb segment of chromosome XI of Saccharomyces cerevisiae. Identification of the known loci URA1, TRP3 and SAC1 revealed a translocation compared to the genetic map. Additionally, six unknown open reading frames have been identified. One of them is similar to catabolic threonine dehydratases. Another one contains characteristic features of membrane transporters. A third one is homologous in half of its length to the prokaryotic hydantoinase HyuA and in the other half to hydatoinase HyuB. A fourth one is homologous to the mammalian phospholipase A₂-activating protein. A fifth one, finally, is homologous to the hypothetical open reading frame YCR007C of chromosome III. The sequence has been deposited in the EMBL data library under Accession Number X75951.     

Complementary case-based reasoning and competitive fuzzy cognitive maps for advanced medical decisions

This paper presents a new hybrid modeling methodology suitable for complex decision making processes. It extends previous work on competitive fuzzy cognitive maps for medical decision support systems by complementing them with case based reasoning methods. The synergy of these methodologies is accomplished by a new proposed algorithm that leads to more dependable advanced medical decision support systems that are suitable to handle situations where the decisions are not clearly distinct. The methodology developed here is applied successfully to model and test two decision support systems, one a differential diagnosis problem from the speech pathology area for the diagnosis of language impairments and the other for decision making choices in external beam radiation therapy.