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1.
An improved method for automatically reconstructing a three-dimensional object from serial cross sections is presented. The method improves the dynamic elastic contour interpolation technique. There are two major improvements: (1) in the case of branching that involves concave contours, instead of pairwise interpolation between the start contour and each goal contour, the goal image is considered globally and local constraints are imposed on the forces exerting upon the vertices; and (2) it takes the continuity of high-order derivatives into consideration and incorporates the schemes of spline theory, quadratic-variation-based surface interpolation, and finite-element-based multilevel surface interpolation to create smoother surfaces of the reconstructed object. Based on the output from the preliminary processing, two alternatives, a quadratic-variation-based surface interpolation algorithm and a finite-element-based multilevel surface interpolation algorithm, can be adopted to obtain the final surface representation 相似文献
2.
The distorted Born iterative method (DBIM) is used to solve two-dimensional inverse scattering problems, thereby providing another general method to solve the two-dimensional imaging problem when the Born and the Rytov approximations break down. Numerical simulations are performed using the DBIM and the method proposed previously by the authors (Int. J. Imaging Syst. Technol., vol.1, no.1, p.100-8, 1989) called the Born iterative method (BIM) for several cases in which the conditions for the first-order Born approximation are not satisfied. The results show that each method has its advantages; the DBIM shows faster convergence rate compared to the BIM, while the BIM is more robust to noise contamination compared to the DBIM. 相似文献
3.
Byrdwell WC 《Lipids》2001,36(4):327-346
Atmospheric pressure chemical ionization (APCI) mass spectrometry (MS) has proven to be a very valuable technique for analysis
of lipids from a variety of classes. This instrumental method readily produces useful ions with gentle fragmentation from
large neutral molecules such as triacylglycerols and carotenoids, which are often difficult to analyze using other techniques.
Molecules that are easily ionized, such as phospholipids, produce molecular ions and diagnostically useful fragment ions that
are complementary to those produced by methods such as electrospray ionization MS with collision-induced dissociation. The
simplicity and versatility of APCI-MS make it an ideal tool for use in solving hitherto very difficult analytical problems. 相似文献
5.
Improved activity and thermostability of Candida antarctica lipase B by DNA family shuffling 总被引:2,自引:0,他引:2
Suen WC Zhang N Xiao L Madison V Zaks A 《Protein engineering, design & selection : PEDS》2004,17(2):133-140
DNA family shuffling was used to create chimeric lipase B proteins with improved activity toward the hydrolysis of diethyl 3-(3',4'-dichlorophenyl)glutarate (DDG). Three homologous lipases from Candida antarctica ATCC 32657, Hyphozyma sp. CBS 648.91 and Crytococcus tsukubaensis ATCC 24555 were cloned and shuffled to generate a diverse gene library. A high-throughput screening assay was developed and used successfully to identify chimeric lipase B proteins having a 20-fold higher activity toward DDG than lipase B from C.antarctica ATCC 32657 and a 13-fold higher activity than the most active parent derived from C.tsukubaensis ATCC 24555. In addition, the stability characteristics of several highly active chimeric proteins were also improved as a result of family shuffling. For example, the half-life at 45 degrees C and melting point (T(m)) of one chimera exceeded those of lipase B from C.antarctica ATCC 32657 by 11-fold and 6.4 degrees C, respectively, which closely approached the stability characteristics of the most thermostable parent derived from Hyphozyma sp. CBS 648.91. 相似文献
6.
本文以北京天文馆的“复杂并形曲面夹层中空玻璃”为例,介绍如何根据玻璃的三雏效果图利用计算机辅助设计来模拟展开空间曲面玻璃。如何绘制切割文件进行玻璃切割;如何根据玻璃的三维效果图利用计算机辅助设计来分割曲面玻璃。进行模具的加工等。 相似文献
7.
Epicardial boundary detection using fuzzy reasoning 总被引:2,自引:0,他引:2
8.
分级无线传感器网络路由协议设计研究 总被引:2,自引:0,他引:2
针对多跳步无线传感器网络(WSN)设计了分级无线传感器网络路由协议框架。协议中级别的设置有效地保证了路径的长度,并且采用最大可用能量优先的决策策略,因此提高了能源的使用效率,网络的使用寿命也得以延长。通过和现有其他协议的仿真比较,该协议的有效性得到了验证。 相似文献
9.
The estimation of dynamically evolving ellipsoids from noisy lower-dimensional projections is examined. In particular, this work describes a model-based approach using geometric reconstruction and recursive estimation techniques to obtain a dynamic estimate of left-ventricular ejection fraction from a gated set of planar myocardial perfusion images. The proposed approach differs from current ejection fraction estimation techniques both in the imaging modality used and in the subsequent processing which yields a dynamic ejection fraction estimate. For this work, the left ventricle is modeled as a dynamically evolving three-dimensional (3-D) ellipsoid. The left-ventricular outline observed in the myocardial perfusion images is then modeled as a dynamic, two-dimensional (2-D) ellipsoid, obtained as the projection of the former 3-D ellipsoid. This data is processed in two ways: first, as a 3-D dynamic ellipsoid reconstruction problem; second, each view is considered as a 2-D dynamic ellipse estimation problem and then the 3-D ejection fraction is obtained by combining the effective 2-D ejection fractions of each view. The approximating ellipsoids are reconstructed using a Rauch-Tung-Striebel smoothing filter, which produces an ejection fraction estimate that is more robust to noise since it is based on the entire data set; in contrast, traditional ejection fraction estimates are based only on true frames of data. Further, numerical studies of the sensitivity of this approach to unknown dynamics and projection geometry are presented, providing a rational basis for specifying system parameters. This investigation includes estimation of ejection fraction from both simulated and real data. 相似文献
10.
A paraboliclike quantum well structure can be used to produce polarization-insensitive waveguide type modulators. We propose the use of interdiffusion of AlGaAs/GaAs quantum wells to achieve the parabolic well shape. Criteria to achieve the paraboliclike quantum wells by interdiffusion are discussed. The theoretical results indicate that interdiffused quantum wells can produce equal eigenstate spacing, polarization insensitive Stark shifts, and modulation similar to an ideal parabolic quantum well. Three procedures are also proposed to develop polarization-insensitive on and off states in paraboliclike interdiffused quantum wells. The modulation depth is compatible with that of the measured parabolic quantum wells. For diffused quantum wells one can take advantage of using an as-grown rectangular quantum well with postgrowth thermal processing. These features demonstrate that an interdiffused quantum well structure can be used to produce a polarization-insensitive electroabsorptive modulator. 相似文献