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Human herpesvirus type 6 DNA derived from human breath was discovered to contaminate PCR reactions during routine reaction preparation. Parallel PCR experiments were conducted in which expiratory secretions were blocked by a surgical mask, while others were performed without any attempt to circumvent respiratory contamination. The experimenter was previously determined to harbor HHV-6 DNA in the saliva. All reactions in which expiration was obstructed were negative for HHV-6 DNA via PCR. Reactions in which there was no attempt to obstruct respiratory secretions were positive for HHV-6 DNA. These data suggest that PCR assays investigating the presence of HHV-6 may be highly susceptible to contamination from the experimenter leading to false positive results.  相似文献   
3.
A country’s economy heavily depends on agricultural development. However, due to several plant diseases, crop growth rate and quality are highly suffered. Accurate identification of these diseases via a manual procedure is very challenging and time-consuming because of the deficiency of domain experts and low-contrast information. Therefore, the agricultural management system is searching for an automatic early disease detection technique. To this end, an efficient and lightweight Deep Learning (DL)-based framework (E-GreenNet) is proposed to overcome these problems and precisely classify the various diseases. In the end-to-end architecture, a MobileNetV3Small model is utilized as a backbone that generates refined, discriminative, and prominent features. Moreover, the proposed model is trained over the PlantVillage (PV), Data Repository of Leaf Images (DRLI), and a new Plant Composite (PC) dataset individually, and later on test samples, its actual performance is evaluated. After extensive experimental analysis, the proposed model obtained 1.00%, 0.96% and 0.99% accuracies on all three included datasets. Moreover, the proposed method achieves better inference speed when compared with other State-Of-The-Art (SOTA) approaches. In addition, a comparative analysis is conducted where the proposed strategy shows tremendous discriminative scores as compared to the various pre-trained models and other Machine Learning (ML) and DL methods.  相似文献   
4.
提出一种基于提升二进小波变换的检测人脸部位(如鼻子、眼睛和嘴唇等)的快速算法。在提升二进小波滤波器中的自由参数是学习的,从便使两个向量(其中一个向量的分量是提升二进小波滤波器系数,另一个向量的分量是人脸某部位像素)之间的夹角的余弦值最大化。学习得到的滤波器应用到测试图像中,可以检测人脸某些部位。实验结果表明,该方法具有较强的鲁棒性,能够很好地解决复杂背景下的人脸检测问题。由于该方法实现的简单性,并容易由硬件实现,使得检测速度进一步提高,因此该方法在可视电话等领域有着广阔的应用前景。  相似文献   
5.
Salmonella serovars are increasing in importance as significant pathogens of both human and animals. Although water and wastewater are treated to eliminate pathogenic microorganisms, they still play an important role in the transmission of Salmonella spp. In this study, bacteriophages infecting Salmonella spp. were isolated from wastewater and evaluated; for their potential to lyse environmental Salmonella strains in vitro at different MOIs and temperatures; and to control the wastewater bacterial community. Three distinct phages designated sww65, sww275, and sww297; as defined by plaque morphology, electron microscopy and host range; were obtained from wastewater. Challenge tests were performed at 37, and 30 °C with the infection of the Salmonella cultures with individual phage, a mixture of two phages, and cocktail of three phages at MOIs of 100, 102, and 104 PFU/CFU. At 30, and 37 °C, a cocktail of three phages reduced all of the Salmonella cultures tested. These results required a high multiplicity of infection. However, when infected with only one phage or a mixture of two phages at MOIs of 100 or 10 2 PFU/CFU, an emergence of bacterial resistance was observed. The dynamic monitoring of wastewater enterobacterial community was conducted using Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR). The number of bands decreased gradually with the use of individual phage or phage cocktails. Moreover, the dynamic monitoring of Salmonella community during wastewater treatment was performed using PCR detection of virulence gene invA. The results correlated with the ERIC-PCR fingerprints, and suggested that Salmonella community was affected by the phage treatment. Indeed, in wastewater, bacteriophages are reducing Salmonella and other members of the Enterobacteriaceae. These results indicated that dynamic changes are closely related with the process of treatment. The introduction of wide host range bacteriophages in wastewater can have a potential impact on the dynamics of the microbial communities, manifested by the reduction or the elimination of microbial species.  相似文献   
6.
This work focuses on the feasibility of reusing and valorising rubber aggregates from shredded worn tyres in cement composites. Our purpose is to study the influence of both microstructures of matrix and of interfacial transition zone (ITZ) on mechanical properties of mortar–rubber aggregates mixtures. The same volume of rubber aggregates substituted volume fractions of sand. Volume ratios of rubber aggregates were ranged from 0% to 50%. Micro structural morphology of various samples was observed by means of scanning electron microscopy (SEM). The matrix porosity and the evolution of hydration products (CSH, CaOH2, CaCO3) were estimated for specimens that had been curing for 28 days at constant temperature and constant relative humidity. An energy dispersive X-ray analyser and X-ray diffraction were used together with SEM in order to quantify these results.  相似文献   
7.
Indium sulfide (In2S3) is a good window or buffer layer for photovoltaic application. In this work, β-In2?xAlxS3 thin films with different thicknesses (400, 442, 646 and 714 nm) are successfully synthesized on heated glass substrates using a chemical spray pyrolysis technique. The thin film thickness effect on the structural, optical and photoluminescence (PL) properties of β-In2?xAlxS3 material is studied. The X-ray diffraction patterns suggest the formation of β-In2S3 cubic phase preferentially oriented towards (400) direction. The level of the residual dislocation seems to be reduced to 3.12 × 109 lines mm?2 for the optimum thickness (646 nm) for which the β-In2?xAlxS3 film crystallinity is the best one. In order to enhance the electrical properties, β-In2?xAlxS3 layers are annealed in air at 400 °C for different annealing times (15, 30 and 45 min). The minimum resistivity, maximum Hall mobility and carrier concentration are found for β-In2?xAlxS3 films annealed for 30 min. All samples have high transmittance of about 75 % but the wide band gap (Eg = 3.32 eV) is obtained for this optimum thickness. This result indicates good optical quality of β-In2?xAlxS3 layers. Defects-related PL properties are also discussed.  相似文献   
8.
Microsystems technology is an emerging technology that is used in ever more technical systems, such as inkjet nozzles or accelerometers. 3D micromechanical structures became feasible by miniaturizing macromechanical elements. One big challenge in downsizing mechanical elements is to adapt conventional manufacturing technologies to the conditions of microtechnology. Integrated design and production processes must be suited to the new requirements, in-depth knowledge of production technology is required to design micromechanical components. Since 2000, the Collaborative Research Center 499 “Design, Production and Quality Assurance of Molded Microcomponents made of Metallic and Ceramic Materials” has been focusing on a continuous and stable process chain for molded micro components (Albers et al. 2005, Microsyst Technol). The aim of prior funding periods was to develop the knowledge about how components, such as gearwheels, and systems, such as micro gears, can be designed. Present research is focused on how to design functional elements, i.e. shaft-hub joints to transmit torque. This paper will introduce an approach that generates know-how via testing several plain bearings and shaft-hub joints in order to derive the necessary know-how to develop simulation tools later on.  相似文献   
9.
Structure of unsteady laminar flow and heat transfer of power-law fluids in two-dimensional horizontal plane channel with a built-in heated square cylinder is studied numerically. The governing equations are solved using a control volume finite element method (CVFEM) adapted to the staggered grid. Computations are performed over a range of Reynolds and Richardson numbers from Re = 20 to 200 and from Ri = 0 to 8, respectively at fixed Prandtl number Pr = 50 and blockage ratio value β′ = 1/8. Three different values of the power-law index (n = 0.5, 1 and 1.4) are considered in this study to show its effect on the value of the critical Reynolds number defining the transition between two different flow regimes (symmetrical and periodic flows), the variations of Strouhal number, drag and lift coefficients and the heat transfer from the square cylinder as function of Reynolds number. Heat transfer correlations are obtained through forced convection. A discussion about the buoyancy effect on the flow pattern and the heat transfer for different power-law index is also presented.  相似文献   
10.
This paper deals with the optimisation of two-levels assembly system planning. This system is composed of a single machine, inventories at levels 1 and 2 for stock keeping components to assembly and final assembled product. It assumed that the machine processes all assembly operations and is subject to random failure. A mathematical model is developed to incorporate a supply planning for two-level assembly systems under stochastic lead times and breakdowns machine. A preventive maintenance plan is carried out to reduce the frequency of the corrective maintenance actions. This work has double goals. The first one is to find the optimal order release dates for the different components at level 2 and the optimal preventive maintenance plan. The second one is to quantify the risk due to machine failures which have an impact on the lead-time of the finished product. To consider the maintenance actions, preventive maintenance actions are perfectly performed to restore the machine to state “as good as new”, minimal repair is considered at failure. The model minimises the total cost, which is the sum of inventory holding cost for components at levels 1 and 2, backlogging and inventory holding cost for the finished products and maintenance costs. To illustrate the effectiveness of the proposed model, different meta-heuristics are applied; the genetic algorithm shows the most suited to our analytical model, the optimal release date founded by this algorithm allows finding the optimal preventive maintenance plan. The obtained optimal maintenance planning is used in the risk assessment in order to find the threshold repair period that avoids lost profit.  相似文献   
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