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Jeong Da-Woon Jeong Hyun-Mo Shin Yu-Jeong Woo Seung-Hye Shim Jae-Hoon 《Food science and biotechnology》2020,29(5):667-674
Food Science and Biotechnology - To determine the physiochemical properties of the 4-α-glucanotransferase from Bifidobacterium sp., the bllj_0114 gene encoding 4-α-glucanotransferase was... 相似文献
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Jong-won Lee Moon-Kyoon Chun Kyung-Min Lee Yu-Jeong Kim Huen Lee 《Korean Journal of Chemical Engineering》2002,19(4):673-678
Understanding the phase behavior and formation kinetics of CO2 hydrate is essential for developing the sequestration process of CO2 into the deep ocean and its feasibility. Three-phase equilibria of solid hydrate, liquid water, and vapor were determined
for aqueous mixtures containing CO2 and NaCl/clay to examine the effect of both ocean electrolytes and sediments on hydrate stability. Due to the capillary effect
by clay pores and inhibition effect by NaCl the corresponding hydrate formation pressure appeared to be a little higher than
that required for simple and pure hydrate at specified temperature. In addition, the hydrate formation kinetics of carbon
dioxide in pure water and aqueous NaCl solutions with or without clay mineral were also measured at various conditions. The
formation kinetic behavior was found to be strongly influenced by pressure, temperature and electrolyte concentration. A simplified
kinetic model having two adjustable parameters was proposed and the estimated results agreed well with the experimental data.
This paper is dedicated to Professor Wha Young Lee on the occasion of his retirement from Seoul National University. 相似文献
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This study was conducted to determine the effects of polyvinyl alcohol (PVA), fetal bovine serum (FBS) and bovine serum albumin (BSA) on blastocoel formation, total cell number, apoptosis and Bcl-xL and Bak gene expression in porcine presumptive diploid parthenotes developing in vitro. The addition of 0.4% BSA to the culture medium enhanced the development of 2-cell or late 4-cell stage parthenotes to the blastocyst stage (P < 0.01) while FBS decreased the incidence of blastocoel formation. FBS also reduced the frequency of blastocysts developed from both 2-cell (P < 0.001) and late 4-cell (P < 0.05) embryos and increased the percentage of blastocysts undergoing apoptosis (P < 0.001). The relative abundance of Bcl-xL mRNA in presumptive diploid parthenotes in the control, PVA- and BSA-supplemented medium was similar to that of in vivo-derived embryos, but was significantly higher than in parthenotes cultured with FBS supplement (P < 0.05). Bak mRNA significantly increased at the blastocyst stage in FBS-supplemented cells (P < 0.01). These results suggest that apoptosis-related gene expression is significantly affected by FBS, and that this may result in alteration of apoptosis and embryo viability of porcine embryos developing in vitro. 相似文献
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The multi-walled carbon nanotube was introduced into the polymer matrix (PANI) to improve the electric conductivity as well as mechanical properties of the original polymer matrix.PANI/multi-walled carbon nanotube (MWCNT) composites were synthesized via ex-situ and in-situ polymerization to improve their electrical property.And the DC conductivities of PANI/MWCNT according to content and diameter of MWCNT were measured by four-point probe.The highest electric conductivity of PANI/MWCNT composite is 20 S/cm when 0.3% (mass fraction) MWCNTs with 10 nm in diameter and 15 μm in length are added in composite. 相似文献
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Jeong-Seob Won Je Young Yeon Hee-Jang Pyeon Yu-Jeong Noh Ji-Yoon Hwang Chung Kwon Kim Hyun Nam Kyung-Hoon Lee Sun-Ho Lee Kyeung Min Joo 《International journal of molecular sciences》2021,22(5)
Stem cell-based therapeutics are amongst the most promising next-generation therapeutic approaches for the treatment of spinal cord injury (SCI), as they may promote the repair or regeneration of damaged spinal cord tissues. However, preclinical optimization should be performed before clinical application to guarantee safety and therapeutic effect. Here, we investigated the optimal injection route and dose for adult human multipotent neural cells (ahMNCs) from patients with hemorrhagic stroke using an SCI animal model. ahMNCs demonstrate several characteristics associated with neural stem cells (NSCs), including the expression of NSC-specific markers, self-renewal, and multi neural cell lineage differentiation potential. When ahMNCs were transplanted into the lateral ventricle of the SCI animal model, they specifically migrated within 24 h of injection to the damaged spinal cord, where they survived for at least 5 weeks after injection. Although ahMNC transplantation promoted significant locomotor recovery, the injection dose was shown to influence treatment outcomes, with a 1 × 106 (medium) dose of ahMNCs producing significantly better functional recovery than a 3 × 105 (low) dose. There was no significant gain in effect with the 3 × 106 ahMNCs dose. Histological analysis suggested that ahMNCs exert their effects by modulating glial scar formation, neuroprotection, and/or angiogenesis. These data indicate that ahMNCs from patients with hemorrhagic stroke could be used to develop stem cell therapies for SCI and that the indirect injection route could be clinically relevant. Moreover, the optimal transplantation dose of ahMNCs defined in this preclinical study might be helpful in calculating its optimal injection dose for patients with SCI in the future. 相似文献
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Ji-Eun Kim Yu-Jeong Roh Yun-Ju Choi Su-Jin Lee You-Jeong Jin Hee-Jin Song A-Yun Seol Hong-Joo Son Jin-Tae Hong Dae-Youn Hwang 《International journal of molecular sciences》2022,23(23)
Tg2576 transgenic mice for Alzheimer’s disease (AD) exhibited significant phenotypes for neuropathological constipation, but no research has been conducted on the association of the fecal microbiota with dysbiosis. The correlation between fecal microbiota composition and neuropathological constipation in Tg2576 mice was investigated by examining the profile of fecal microbiota and fecal microbiota transplantation (FMT) in 9–10-month-old Tg2576 mice with the AD phenotypes and constipation. Several constipation phenotypes, including stool parameters, colon length, and histopathological structures, were observed prominently in Tg2576 mice compared to the wild-type (WT) mice. The fecal microbiota of Tg2576 mice showed decreases in Bacteroidetes and increases in the Firmicutes and Proteobacteria populations at the phylum level. The FMT study showed that stool parameters, including weight, water content, and morphology, decreased remarkably in the FMT group transplanted with a fecal suspension of Tg2576 mice (TgFMT) compared to the FMT group transplanted with a fecal suspension of WT mice (WFMT). The distribution of myenteric neurons and the interstitial cells of Cajal (ICC), as well as the enteric nervous system (ENS) function, remained lower in the TgFMT group. These results suggest that the neuropathological constipation phenotypes of Tg2576 mice may be tightly linked to the dysbiosis of the fecal microbiota. 相似文献
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Yu-Jeong Shin Won-Hee You Hyun-Moo Hur Joon-Hyuk Park 《Journal of Mechanical Science and Technology》2012,26(11):3423-3431
As train operating speeds increase, upgrading the performance of the railway vehicle has been required. To improve the ride comfort related to vibration, researches to replace the conventional passive suspension with (semi)active suspension have been carried out. For the purpose of vibration reduction, the magneto-rheological (MR) damper has been widely applied in automobile or anti-vibration areas, but it hasn??t yet been applied to railway vehicles. In this study, skyhook control with the MR damper was applied to the 1/5 scaled railway vehicle model on roller rig to suppress the lateral vibration of the carbody. For the 1/5 scaled prototype model of the railway vehicle, the dimensions of railway vehicle were scaled down by similarity method. Both numerical analysis and experiments to control the carbody lateral vibration were conducted and compared. The possibility of improving both the ride comfort and running stability of the railway vehicle by using the semi-active control was discussed in detail. 相似文献
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In a common salt-in-polymer electrolyte, a polymer which has polar groups in the molecular chain is necessary because the
polar groups dissolve lithium salt and coordinate cations. Based on the above point of view, polystyrene [PS] that has nonpolar
groups is not suitable for the polymer matrix. However, in this PS-based composite polymer-in-salt system, the transport of
cations is not by segmental motion but by ion-hopping through a lithium percolation path made of high content lithium salt.
Moreover, Al2O3 can dissolve salt, instead of polar groups of polymer matrix, by the Lewis acid-base interactions between the surface group
of Al2O3 and salt. Notably, the maximum enhancement of ionic conductivity is found in acidic Al2O3 compared with neutral and basic Al2O3 arising from the increase of free ion fraction by dissociation of salt. It was revealed that PS-Al2O3 composite solid polymer electrolyte containing 70 wt.% salt and 10 wt.% acidic Al2O3 showed the highest ionic conductivity of 9.78 × 10-5 Scm-1 at room temperature. 相似文献
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