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排序方式: 共有120条查询结果,搜索用时 15 毫秒
1.
Akira Minami Yuuki Kurebayashi Tadanobu Takahashi Tadamune Otsubo Kiyoshi Ikeda Takashi Suzuki 《International journal of molecular sciences》2021,22(6)
Sialidase cleaves sialic acid residues from glycans such as glycoproteins and glycolipids. In the brain, desorption of the sialic acid by sialidase is essential for synaptic plasticity, learning and memory and synaptic transmission. BTP3-Neu5Ac has been developed for sensitive imaging of sialidase enzyme activity in mammalian tissues. Sialidase activity in the rat hippocampus detected with BTP3-Neu5Ac increases rapidly by neuronal depolarization. It is presumed that an increased sialidase activity in conjunction with neural excitation is involved in the formation of the neural circuit for memory. Since sialidase inhibits the exocytosis of the excitatory neurotransmitter glutamate, the increased sialidase activity by neural excitation might play a role in the negative feedback mechanism against the glutamate release. Mammalian tissues other than the brain have also been stained with BTP3-Neu5Ac. On the basis of information on the sialidase activity imaging in the pancreas, it was found that sialidase inhibitor can be used as an anti-diabetic drug that can avoid hypoglycemia, a serious side effect of insulin secretagogues. In this review, we discuss the role of sialidase in the brain as well as in the pancreas and skin, as revealed by using a sialidase activity imaging probe. We also present the detection of influenza virus with BTP3-Neu5Ac and modification of BTP3-Neu5Ac. 相似文献
2.
Zengyun Jian Kosuke Nagashio Kazuhiko Kuribayashi 《Metallurgical and Materials Transactions A》2002,33(9):2947-2953
Using an electromagnetic levitation facility with a laser heating unit, silicon droplets were highly undercooled in the containerless
state. The crystal morphologies on the surface of the undercooled droplets during the solidification process and after solidification
were recorded live by using a high-speed camera and were observed by scanning electron microscopy. The growth behavior of
silicon was found to vary not only with the nucleation undercooling, but also with the time after nucleation. In the earlier
stage of solidification, the silicon grew in lateral, intermediary, and continuous modes at low, medium, and high undercoolings,
respectively. In the later stage of solidification, the growth of highly undercooled silicon can transform to the lateral
mode from the nonlateral one. The transition time of the sample with 320 K of undercooling was about 535 ms after recalescence,
which was much later than the time where recalescence was completed. 相似文献
3.
Mingjun Li Kosuke Nagashio Ph.D. Kazuhiko Kuribayashi 《Metallurgical and Materials Transactions A》2002,33(8):2677-2683
A mullite (3Al2O3·2SiO2) sample has been levitated and undercooled in an aero-acoustic levitator, so as to investigate the solidification behavior
in a containerless condition. Crystal-growth velocities are measured as a function of melt undercoolings, which increase slowly
with melt undercoolings up to 380 K and then increase quickly when undercoolings exceed 400 K. In order to elucidate the crystal
growth and solidification behavior, the relationship of melt viscosities as a function of melt undercoolings is established
on the basis of the fact that molten mullite melts are fragile, from which the atomic diffusivity is calculated via the Einstein-Stokes equation. The interface kinetics is analyzed when considering atomic diffusivities. The crystal-growth
velocity vs melt undercooling is calculated based on the classical rate theory. Interestingly, two different microstructures are observed;
one exhibits a straight, faceted rod without any branching with melt undercoolings up to 400 K, and the other is a feathery
faceted dendrite when undercoolings exceed 400 K. The formation of these morphologies is discussed, taking into account the
contributions of constitutional and kinetic undercoolings at different bulk undercoolings. 相似文献
4.
C Nishiyama M Fukada Y Usui N Iwamoto T Yuuki Y Okumura H Okudaira 《Canadian Metallurgical Quarterly》1995,32(14-15):1021-1029
Der f 2 is a major mite allergen composed of 129 amino acid residues. To determine the major epitopes on Der f 2 recognized by human IgE antibodies, artificial mutations were introduced to Der f 2 protein. The IgE-binding activity of Der f 2 was significantly decreased by deletion of 10 amino acids at the N-terminus or nine amino acids at the C-terminus. Site-directed mutagenesis with a single amino acid replacement by Ala or Leu in both N- and C-terminal regions as well as a central portion was performed to generate 42 single-site mutations. Amino acid replacement around a disulfide bond of Cys8-Cys119 caused a marked decrease in IgE-binding activity. Furthermore, a distinct decrease in IgE-binding was also caused by Ala-substitution close to a disulfide bond of Cys73-Cys78 and by mutations of a few charged residues. From these results, it was concluded that the two disulfide-forming regions of Der f 2 and several charged residues are important for forming major epitope structures recognized by human IgE antibodies. 相似文献
5.
Containerless processing was used to investigate the glass-forming behavior of Al2 O3 –Y2 O3 glass. The amorphous bulk samples were obtained at compositions with 25–37.5 mol% yttria when the melt was cooled at a cooling rate of ∼250 K/s. Although small spherical particles (∼10 μm) with the same composition of the matrix were detected in the amorphous samples with 32.5–37.5 mol% yttria, the microfocus X-ray diffraction result indicated that the small spherical particles were crystalline Y3 Al5 O12 garnet (YAG), rather than being amorphous. This observation suggested that small YAG particles could not grow larger after their nucleation, because of the high viscosity at high undercooling and the high cooling rate, which would graze the nose of the continuous cooling temperature diagram of YAG. 相似文献
6.
The time dependency of the amounts of corrosion products and co-existing adsorbed water on copper in humid air containing SO2 was estimated from a series of in situ time-resolved IR-RAS spectra on the basis of the relations between the band intensities and the mass changes of the corrosion products, which were determined by simultaneous measurement of IR-RAS and QCM. The amounts of both corrosion products increased slowly at the initial stage and later increased rapidly. Although the relative humidity was kept constant, the amount of adsorbed water increased nearly the same behavior as that of corrosion products in the stage of relatively small amounts of corrosion products and later increased rapidly when the amounts of corrosion products increased. In humid air without SO2, sulfite gradually decomposed and some of it changed into sulfate. 相似文献
7.
A DC (data center) demands air-conditioning power as large as the 1/3-1/2 of total electricity consumption. Thus, energy saving of cooling power of DC yields considerable effect on both economic and environmental views. PV (Photovoltaic) and absorption refrigerator with CGS (cogeneration systems) or gas boiler are possible power saving options. The waste warm air from DC would be utilized for greenhouse heating when DC and greenhouse locate near in the suburbs. In this study, the authors develop an energy network model to assess the potential contribution of DC as a major electric power and chilled air consumer as well as the warm air supplier in a district to the energy efficiency improvement. The evaporation heat of LNG (liquefied natural gas) utilization is also considered as well as PV, CGS. This model is applied to the cases of the urban area in Tokyo which involves athletic center, shops and hospital and the suburbs including greenhouse and then compared. 相似文献
8.
A design of high optical efficiency wedge type light guide plate adopting twin inclined cylindrical surface 下载免费PDF全文
Kenji Itoga Kazutada Takaira Seiji Sakai Toshiaki Fujino Tetsuya Satake Akimasa Yuuki 《Journal of the Society for Information Display》2017,25(8):504-514
We propose wedge type light guide plate (LGP) adopting twin inclined cylindrical surfaces (TICS) at the incident part of a LGP. It has higher optical efficiency than conventional wedge type LGP. In dimension of LGP which the thickness of incident part is 1.5 mm and that of luminance part is 0.7 mm, the optical efficiency of wedge type LGP adopting TICS is expected to be 96% in comparison with a normal flat LGP. In addition, there is an advantage to reduce the non‐uniformity of vicinity of light emitted diodes (LEDs) by TICS reflecting light laterally. However, it is necessary that the LED centers are aligned with the intersections of TICS. The misalignment of LED causes non‐uniformity of optical profile that is slightly larger than a normal LGP. 相似文献
9.
10.
Y. Inatomi F. Onishi K. Nagashio K. Kuribayashi 《International Journal of Thermophysics》2007,28(1):44-59
The density and thermal conductivity of a high-purity silicon melt were measured over a wide temperature range including the
undercooled regime by non-contact techniques accompanied with electromagnetic levitation (EML) under a homogeneous and static
magnetic field. The maximum undercooling of 320 K for silicon was controlled by the residual impurity in the specimen, not
by the melt motion or by contamination of the material. The temperature dependence of the measured density showed a linear
relation for temperature as: ρ(T) = 2.51 × 103−0.271(T−T
m) kg · m−3 for 1367 K < T < 1767 K, where T
m is the melting point of silicon. A periodic heating method with a CO2 laser was adopted for the thermal conductivity measurement of the silicon melt. The measured thermal conductivity of the
melt agreed roughly with values estimated by a Wiedemann–Franz law. 相似文献