Molecular Cloning,Structural Analysis and Tissue Expression of Protein Phosphatase 3 Catalytic Subunit Alpha Isoform (PPP3CA) Gene in Tianfu Goat Muscle

Calcineurin, a Ca²⁺/calmodulin-dependent protein phosphatase, plays a critical role in controlling skeletal muscle fiber type. However, little information is available concerning the expression of calcineurin in goat. Therefore, protein phosphatase 3 catalytic subunit alpha isoform (PPP3CA) gene, also called calcineurin Aα, was cloned and its expression characterized in Tianfu goat muscle. Real time quantitative polymerase chain reaction (RT-qPCR) analyses revealed that Tianfu goat PPP3CA was detected in cardiac muscle, biceps femoris muscle, abdominal muscle, longissimus dors muscle, and soleus muscle. High expression levels were found in biceps femoris muscle, longissimus muscle and abdominal muscle (p < 0.01), and low expression levels were seen in cardiac muscle and soleus muscle (p > 0.05). In addition, the spatial-temporal mRNA expression levels showed different variation trends in different muscles with the age of the goats. Western blotting further revealed that PPP3CA protein was expressed in the above-mentioned tissues, with the highest level in biceps femoris muscle, and the lowest level in soleus muscle. In this study, we isolated the full-length coding sequence of Tianfu goat PPP3CA gene, analyzed its structure, and investigated its expression in different muscle tissues from different age stages. These results provide a foundation for understanding the function of the PPP3CA gene in goats.     

基于微水振荡试验资料确定含水层渗透系数

岩土体渗透性参数是评价地下水资源,研究地下水运动规律的重要水文地质参数。微水试验相较于传统的测试方法是一种现场快速测定岩土体渗透性参数的方法,其试验时间短,对地下水及周边环境影响甚微,具有广泛的应用前景。该文采用两种微水试验的Kipp(1985)模型和Cooper(1967)模型对现场试验结果进行了计算分析,并将计算结果与传统抽水试验成果进行对比分析,两种试验成果拟合较好,表明微水试验测试方法可用于测定介质的渗透系数。     

Reduction of the Criteria System for Identifying Effective Reservoirs in the Joint Operation of a Flood Control System

Water Resources Management - The accurate and efficient identification of effective reservoirs plays an important role in the real-time flood control operation of multireservoir systems. The...     

LD端面泵浦Nd:YVO4激光器热管理结构的研究

基于一种LD端面泵浦Nd:YVO<,4>激光器,设计了一种热沉结合外部风扇散热的风冷式热管理结构,并利用有限元分析方法对Nd:YVO<,4>晶体的温度场进行了数值模拟,分析了环境温度及界面热阻变化对晶体热致效应的影响.模拟结果表明,晶体泵浦区的温度梯度约为17 ℃,当环境温度升高时,所设计的冷却方案仍能满足系统的散热需...     

粮油食品综合实验楼箱基地下室的防水施工及问题探讨

地下室防水工程质量好坏,直接影响到工程的寿命和各种设备的正常使用.目前,有不少地下室防水效果不理想,存在渗漏水现象.根据不同情况,寻找有效防治措施,对保证工程质量有一定现实意义.在粮油食品综合实验楼地下室防水工程施工中,我们采用了混凝土自防水.加之素灰、水泥砂浆防水层做法,经过去年一个雨季的实践观察,未发现渗漏现象,收到了良好效果.     

“互联网+教育”在我校《无机与分析化学》课程中的改革与实践

将“互联网+教育”应用到我校《无机与分析化学》课程的教学过程中,通过建立“班级课程群”保障“互联网+德育”、“互联网+线上线下混合教学”的实施,找到了我校《无机与分析化学》课程在进行“互联网+教育”过程中亟待解决的问题和发展方向,推动了我校现代化信息技术与教学的深度融合。     

数字媒体技术影响下博览建筑中沉浸式展览空间的设计策略

随着科学技术的进步和经济社会高速发展,沉浸式展览也由传统媒介走向混媒介,进而抵达现代数字媒介的阶段.作为一个科学与人文艺术紧密结合的表现形式,沉浸式展览应当从更多维度全面、深刻地被理解.基于数字媒体影响下博览建筑中沉浸式展览空间设计需求,该文分别从以人为本的建筑空间尺度划分、数字媒体技术融入建筑空间界面和博览建筑空间物...     

低压煤气管网支管设计计算探讨

本文以两种典型的计算方法求出小区供气管网的管径,作技术经济指标分析,并运用计算机模拟运行的方法得到管网运行的节点压力分布(或用户灶前压力),比较它们的优劣。认为根据实际情况选用合适的管内粗糙度和计算内径,并适当考虑压头余量,用全压法作设计计算是最好的设计计算方法。     

Water Resources Allocation in Transboundary River Basins Based on a Game Model Considering Inflow Forecasting Errors

Dynamic transboundary water resources allocation based on inflow prediction results is an important task for water resources management in river basins. This paper takes the watershed management agency as the leader and the associated area as the follower, and proposes a two-level asymmetric Nash-Harsanyi Leader-Follower game model considering inflow forecasting errors. In the proposed model, the Monte Carlo method is used to analyze the uncertainty of various stakeholder allocation results and the response regularity to the total water resource uncertainty. The quantitative relationship between the allocation results of stakeholders and the mean and standard deviation of total water resource uncertainty is subsequently established. The Huaihe River basin in China is selected as a case study. The results show the following: (1) the water allocated to the watershed management agency and three provinces has a normal distribution when the inflow forecasting error obeys the normal distribution; (2) the sum of the mean of the water allocated to stakeholders equals the mean of the forecast water resource and the sum of the standard deviations of the water allocated to stakeholders equals the standard deviation of the forecast water resource; (3) the mean and standard deviation of the allocation results have a good linear relationship with the mean and standard deviation of forecast water resource; (4) the distribution parameters of the stakeholder allocation results can be directly derived from the distribution parameters of the forecast information, thus aiding the stakeholders in making decisions and improving the practical value of the method.

     

Control of the structure and mechanical property of porous WS<Subscript>2</Subscript> scaffold during freeze casting

Porous WS₂ scaffolds with aligned lamellar pore were fabricated by freeze casting, and the pore morphology and size can be well controlled by adjusting the processing parameters during freeze casting process. The results indicated that the porosity and the compressive strength of porous WS₂ were greatly affected by the concentration of gelatin. As the gelatin addition increased from 1 to 5 wt%, the porosity of porous WS₂ scaffolds with initial solid content of 3 vol% decreased from 95.12 to 90.23%, and their compressive strength increased from 0.22 to 1.16 MPa. Moreover, the lamellar spacing and wall thickness could be tailored from 90 to 320 and 5 to 30 μm respectively by changing the cooling temperature. And the compressive strength of scaffolds has a slight increase with the decreases of cooling temperature. The porous WS₂ scaffold with fine aligned lamellar structure and proper compressive strength are expected to be used for scaffold materials.