Identification of Host Cellular Protein Substrates of SARS-COV-2 Main Protease

The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease-19 (COVID-19) being associated with severe pneumonia. Like with other viruses, the interaction of SARS-CoV-2 with host cell proteins is necessary for successful replication, and cleavage of cellular targets by the viral protease also may contribute to the pathogenesis, but knowledge about the human proteins that are processed by the main protease (3CLpro) of SARS-CoV-2 is still limited. We tested the prediction potentials of two different in silico methods for the identification of SARS-CoV-2 3CLpro cleavage sites in human proteins. Short stretches of homologous host-pathogen protein sequences (SSHHPS) that are present in SARS-CoV-2 polyprotein and human proteins were identified using BLAST analysis, and the NetCorona 1.0 webserver was used to successfully predict cleavage sites, although this method was primarily developed for SARS-CoV. Human C-terminal-binding protein 1 (CTBP1) was found to be cleaved in vitro by SARS-CoV-2 3CLpro, the existence of the cleavage site was proved experimentally by using a His₆-MBP-mEYFP recombinant substrate containing the predicted target sequence. Our results highlight both potentials and limitations of the tested algorithms. The identification of candidate host substrates of 3CLpro may help better develop an understanding of the molecular mechanisms behind the replication and pathogenesis of SARS-CoV-2.     

Formation and growth mechanism of TiC crystal in TiC_p/Ti composites

1　ＩＮＴＲＯＤＵＣＴＩＯＮＰａｒｔｉｃｌｅｓｒｅｉｎｆｏｒｃｅｄｔｉｔａｎｉｕｍａｌｌｏｙｍａｔｒｉｘｃｏｍｐｏｓｉｔｅｓ(ＴＭＣｐ)ｈａｖｅｗｉｄｅａｐｐｌｉｃａｔｉｏｎｆｉｅｌｄｓｆｏｒｔｈｅｉｒａｔｔｒａｃｔｉｖｅｈｉｇｈｓｐｅｃｉｆｉｃｓｔｒｅｎｇ     

Social environmental selection as a mediator of gender, ethnic, and personality effects on college student drinking.

Structural equation modeling was used to test the mediational role of social environmental selection on alcohol use in cross-sectional samples of 447 students from a rural state university and 421 students from an urban private university. Results showed that male gender, White ethnicity, and sensation seeking were uniquely associated with greater alcohol use. Mediational analyses indicated that socioenvironmental factors (i.e., Greek involvement, friends' approval of drinking/getting drunk) were positively associated with alcohol use and significantly accounted for parts of the effects of ethnicity and sensation seeking, but not gender, on alcohol use. Results suggest that White students and those high on sensation seeking may drink more heavily in college, in part because they select social environments in which alcohol use is encouraged. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

W-35%Cu液相活化烧结工艺研究

本文从钨铜两相迁移机制和固溶扩散的角度 ,实验研究了钨铜复合粉的活化和常规烧结 ,分析了钨铜复合粉液相活化烧结过程中球磨时间、烧结温度、保温时间和升温速度对材料致密度的影响。结果表明 ,短时间球磨的钨铜复合粉具有良好的烧结性能 ,烧结温度和保温时间对材料致密度影响很大 ,活化烧结能有效降低烧结温度 ,提高烧结致密度     

A heuristic algorithm for distributed control in manufacturing systems

Distributed Problem Solving Networks (DPSN) provide a means for interconnecting intelligent problem solver nodes that can solve only a part of a problem depending on their ability in the problem domain. The decomposition of a problem into subproblems, and the selection of nodes to solve them can be regarded as the generation of an AND/OR tree, and the solution of the problem as a search for a solution tree. Introducing measurements for the cost of a solution tree, we present an algorithm to find one having minimal cost under certain conditions. A Flexible Manufacturing System consisting of a network of flexible workcells is used as an example.     

A controllable chaotic system based on inductively coupled bistable oscillators

A mathematical model of a new controllable autooscilatory chaotic system based on inductively coupled Chua’s oscillators is described. Numerical simulations show that, using a chaotizing feedback algorithm, chaotic oscillations in this system can be excited in regimes where only regular oscillations are generated otherwise.     

国内外钻探用金刚石复合片性能的研究

主要介绍国内外金刚石复合片性能及采用四高复合片钻探硬岩的初试,为快速钻探提供一个新的途径.     

A method for multi-parameter PDF estimation of random variables

The probability distribution function (PDF) of a random variable Z is approximated with c·e^Q(z), where Q(z) is a polynomial function and c is normalizing constant. Based upon the weighted residual method, general linear algebraic equations have been derived for the evaluation of the unknown parameters in the polynomial. Numerical examples are presented and the results show that the PDFs obtained using the proposed method converge to those obtained from Monte Carlo simulation as the number of parameters in the approximate PDF increases.     

真空热处理工艺参数的选取

通过多种材料、多种工件、多批次的加工，对常见真空热处理工艺参数的确定，摸索出一些经验公式，并在实际生产中取得很好效果。