化学法和机械法从大肠杆菌释放人肿瘤坏死因子

对化学法和机械法破碎基因工程大肠杆菌以释放人肿瘤坏死因子的影响因素作了考察。在化学法破菌中研究了破碎时间、试剂浓度、溶剂ｐＨ值、加入表面活性剂等因素的影响。在机械法破碎中研究了停留时间、装珠量、搅拌转速、细胞浓度等的影响，对操作条件进行了优化。通过对二者的比较，发现机械法破碎细胞效率高，但产生细小的碎片不利后分离；化学法释放产物有选择性，但操作时间长。     

裂缝性致密砂岩气层暂堵性堵漏钻井液技术

钻井液漏失是目前制约裂缝性致密砂岩气藏勘探开发的第一位因素，以往的工程实践对堵漏工艺及工程成功率关注较多，而对堵漏材料进入储层带来的储层损害以及如何高效解除则关注较少。堵漏材料一旦进入储层若不及时解除，就会造成严重的储层损害。为此，以“储层保护屏蔽暂堵技术思想”为指导，在评价常用堵漏材料酸溶性的基础上，提出集成惰性堵漏材料架桥与酸溶性堵漏材料填充优势的暂堵性堵漏钻井液技术新思路。通过室内探索性实验，优化了暂堵性堵漏钻井液配方，结果显示：基浆中酸溶性材料与惰性架桥材料加量的比值介于1∶1～3∶1之间为最佳。由此形成了暂堵性堵漏钻井液漏失控制技术，并通过工程实践证明了其合理性。     

Simultaneous Cell Disruption and Aqueous Two-Phase Extraction for Isolation of Intracellular Recombinant Proteins

A new technique was developed for the integrated processing of cell disruption and aqueous two-phase extraction in a high-speed bead mill to separate intracellular proteins form microbial cells. The process was narned as simultanecus cell disruption and aqueous two-phase extraction (ADATE). Advantages, such as high cell disruption efficiency, biochemical activities proservation of proteins, cell debris elimination, and preliminary puriffcation of the target protein were being clairmed. When this technique was employed for isolating recombinant Tumor Necrois Factor (TNF) from E.coli, overall protein codcentration and TNF activity were found to have been increased. More than 85% of TNF was partitioned into the top phase and all cell debris were in the bottom phase. The partition coefficinet was greater than 3 and the TNF puriflcation fsctop was greater than 6. It is zhown that less separation steps were being utilized in the new techniqne, meaning a reduction in separation time and less process extractors required.     

川中莲池大安寨油藏渗吸注水开发的研究及应用

川中莲池大安寨油藏具有低孔、低渗和非均质性特征。开采难度大,一次采收率低。在室内实验研 究的基础上实施了渗吸注水开发,取得了一定的效果,既补充了地层能量又增加了产量。同时形成了一套切合川 中实际的注水配套工艺技术、动态监测技术和水处理及监测技术。为川中下一步扩大在其桂花和公山庙油田的注 水开发提供了宝贵的经验。