Determination of the depletion of furazolidone residues in chicken tissues using a Bacillus stearothermophilus test

This study assessed the applicability of a Bacillus stearothermophilus test for detection of the depletion of furazolidone anticoccidial drug residues in chicken tissues. Thirty-three Ross breed chicken were dosed orally with furazolidone (2 mg/kg body weight) daily for 5 days. After the last treatment the birds were sacrificed in groups of three at intervals of 1, 3, 6, 10, 24, 48, 144, 240, 360 and 480 h. Liver, kidney and breast muscle samples were collected and immediately analysed at each interval for furazolidone residues. Serum was separated from the blood collected from jugular veins into bottles containing glass beads and analysed. Supernatant from the homogenates was analysed for the presence or absence of furazolidone residues using the B. stearothermophilus test. Semi quantification of furazolidone residues was done by comparing the obtained zones of inhibition on a Bacillus subtilis test plates and the zones from calibrated standard curves. Qualitatively the B. stearothermophilus test was positive for furazolidone residues up to 480 h post treatment in serum samples, 360 h in liver samples, 480 h in kidney samples and 360 h in muscle samples. Semi quantitatively from the B. subtilis test plates, serum samples were observed to have the highest furazolidone concentration of 3.2 μg/ml. The lowest furazolidone concentration was recorded in kidney tissues at 0.21 μg/ml. Kidney and serum tissues appear to be good tissues for testing of furazolidone tissues. The Kidney samples would appear best for post-mortem screening of furazolidone residues while serum could be used antemortem screening of depletion in chicken.     

Factors contributing to the occurrence of antimicrobial drug residues in Kenyan milk

The study investigated factors contributing to the occurrence of antimicrobial drug residues in milk within four major milk production districts in Kenya. The frequency of contamination was studied among small- and large-scale dairy producers to determine if there were differences between the two types of producers. Field samples (n = 1,600) were analyzed with the improved Dutch tube diffusion test, a microbial inhibitor test (Bacillus stearothermophilus). In total, 144 and 64 samples from small- and large-scale producers, respectively, were found to contain beta-lactam antibiotics at levels exceeding the established Codex maximum residue level for penicillin G (4 microg/kg). The difference in results between the two categories of producers was found to be significant (P < 0.001). To explain the higher frequency of antibiotic contamination of milk from small-scale producers, a questionnaire was constructed and used with 220 randomly selected smallholders in the selected districts. The results suggested (i) lack of understanding of risks related to antibiotic contamination of food, (ii) poor or no treatment records, and (iii) lack of a monitoring system as major risks for contamination. It was concluded that intensification of the education among small-scale dairy producers would greatly reduce the occurrence of antimicrobial residues in milk.     

Prevalence of multidrug resistant Staphylococcus aureus in milk from large- and small-scale producers in Kenya

This study evaluated the prevalence of multidrug resistant Staphylococcus aureus in Kenyan milk and investigated any differences in antimicrobial resistance between large- (>200 L/d) and small- (<50 L/d) scale producers. Susceptibility profiles for penicillin G, tetracycline, erythromycin, trimethoprim/sulfamethazine, and chloramphenicol were determined for Staph. aureus (n=402) isolated from cows with subclinical mastitis. There was a significant difference in the overall mean resistance profile between large- (7.1%) and small-scale farm (14.7%) isolates. The overall prevalence of multidrug resistance (> or =2 antibiotics) differed significantly between isolates from small farms (34.3%) and those from large farms (18.0%). Additionally, the producers were interviewed about their usage of antimicrobial drugs and their attitudes toward education in related fields. There was an evident difference between the producer types in their documentation of the use of antimicrobial drugs. Small-scale farms were less inclined to documentation, and treatment records were available from 22% of small-scale farms, compared with 73% of large-scale farms. Farmers expressed a need for more information in 5 areas, ranking preventive management highest (34.0%); followed by affordable tests to control residues in milk (22.8%); preparation of antimicrobial drugs (20.0%); public health concerns (11.2%); disposal of surplus antimicrobial drugs (7.8%); and antimicrobial drug residue persistence in milk (4.2%). It was concluded that herd size might be an indirect risk factor in the development of antimicrobial resistance in Staph. aureus within the region.     

Changes in physico-chemical and functional properties during convective drying of aloe vera (Aloe barbadensis) leaves

Aloe vera leaves were dried at different temperatures in hot air oven and powdered. The percent powder yield was found 2.60%, 2.60%, 2.55% and 2.52% at 50, 60, 70 and 80 °C respectively. Powder samples had the pH (1% solution) 3.51, 3.53, 3.52 and 3.53 with the rise of drying temperature in the selected range. Statistically, yield and pH indicated no significant difference (p < 0.5) due to drying temperature variation. Wettability of powder at 70 °C was 32 s as compared to 35, 35 and 37 s in the samples obtained at 50, 60 and 80 °C respectively. Water absorption capacity of powder at 70 °C was 359% as compare to 351%, 354% and 356% of 50, 60, and 80 °C powder samples. The HPLC chromatogram obtained for the sample dried at 80 °C shows that as the temperature increased from 50 to 80 °C, aloin content decreased from 10.6 to 1.7 ppm. The “a” values were found 2.028, 2.226, ?0.282 and 2.531 for the samples obtained after drying at 50, 60, 70 and 80 °C respectively. Samples obtained at 70 °C showed negative “a” value indicated that the sample was more greenish in colour as compared to other samples.     

Catechins depletion patterns in relation to theaflavin and thearubigins formation

The determination of the depletion pattern of catechins in black tea processing is important in achieving optimum tea quality. This study investigated catechins (unoxidised di- and trihydroxylated) depletion patterns in relation to theaflavin and thearubigin formation. It was during the process of green leaf fermentation at selected temperature and time combinations. The tea leaves were obtained from three clones (6/8, 303/577 and 311/287) within the Tea Research Foundation of Kenya. The results were showed that unequal depletion rates of di- and trihydroxylated catechins led to a decline in total theaflavin and an increase in thearubigins levels. An equitable decline in both groups of catechins corresponded to a subsequent rise in theaflavins content. The decline in the catechins levels was much faster at higher temperatures resulting in a shorter fermentation time to achieve a peak of the theaflavins content. Clone 311/287 had the highest mean theaflavins content (26.99 μmol/g) and the least mean percent thearubigins (15.02%) level. Theaflavins content correlated positively with liquor brightness determined by a spectrophotometer and tea tasters (r = 0.7221, p < .0001). The thearubigins content was however found to relate negatively with liquor brightness. It was concluded that the experimental conditions tested form a good basis for clonal specific processing conditions that can be utilised in manufacturing quality black tea.