NUCLEAR MAGNETIC RESONANCE STUDIES OF ASPHALTENES AND COALS. A NEW APPROACH TO OBTAINING ASPHALTENE STRUCTURAL PARAMETERS WITH DIPOLAR DEPHASING

Apparent carbon aromaticities, fa, of some asphaltenes have been measured by n.m.r. in solution and compared with those obtained by the CP/MAS technique on solid samples. The aromaticities were also measured for several Alberta plains coals. From the 1H solution spectra and 13C solid nmr spectra of asphaltenes, some of their skeletal features, not obtainable from solution spectra only, have been suggested.     

Optically pumped rubidium as a frequency standard at 196 THz

The performance of 196.0-THz (1529-nm) DFB lasers frequency-locked to absorption lines of a rubidium vapor optically pumped at 384.2 THz (780.2 nm) is studied. The absorption profiles of the pumped vapor are measured under various conditions and compared with theoretical predictions. A bright resonance resulting from the cascade of two cycling transitions is characterized both experimentally and theoretically. The measured frequency stability of a DFB laser frequency-locked to this line reaches a level of 2×10^-10 for an averaging time of 100 s when compared to a similar laser locked to an acetylene line     

Assessment of Hematological and Biochemical parameters with extended D-Ribose ingestion

D-ribose, a naturally occurring pentose carbohydrate, has been shown to replenish high- energy phosphates following myocardial ischemia and high intensity, repetitive exercise. Human studies have mainly involved short-term assessment, including potential toxicity. Reports describing adverse effects of D-ribose with prolonged ingestion have been lacking. Therefore, this study assessed the toxicity of extended consumption of D-ribose in healthy adults. Nineteen subjects ingested 20 grams/Day (10 grams, twice a Day) of ribose with serial measurements of biochemical and hematological parameters at Days 0, 7, and 14. No significant toxic changes over the 14-day assessment period occurred in complete blood count, albumin, alkaline phosphatase, gamma glutamyltransferase, alanine amiotransferase, and aspartate aminotransferase. However, D-ribose did produce an asymptomatic, mild hypoglycemia of short duration. Uric acid levels increased at Day 7, but decreased to baseline values by Day 14. D-ribose consumption for 14 days appears not to produce significant toxic changes in both hematological and biochemical parameters in healthy human volunteers.     

Gender IQ differences among psychiatric patients.

Provides evidence against the hypothesis that adult male psychiatric patients are more intelligent than their female peers. 1,187 patients were administered either the Wechsler-Bellevue Intelligence Scales, the Wechsler Adult Intelligence Scale (WAIS), the Wechsler Intelligence Scale for Children—Revised (WISC—R), or the Wechsler Adult Intelligence Scale—Revised. Analyses revealed no significant sex differences except on the WISC—R. Explanations that have been suggested to account for previous observations of discrepant IQ test performance are discussed, and the possibility of regional or institutional biases is noted. (French abstract) (19 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

MiR-10a in Pancreatic Juice as a Biomarker for Invasive Intraductal Papillary Mucinous Neoplasm by miRNA Sequencing

New biomarkers are needed to further stratify the risk of malignancy in intraductal papillary mucinous neoplasm (IPMN). Although microRNAs (miRNAs) are expected to be stable biomarkers, they can vary owing to a lack of definite internal controls. To identify universal biomarkers for invasive IPMN, we performed miRNA sequencing using tumor-normal paired samples. A total of 19 resected tissues and 13 pancreatic juice samples from 32 IPMN patients were analyzed for miRNA expression by next-generation sequencing with a two-step normalization of miRNA sequence data. The miRNAs involved in IPMN associated with invasive carcinoma were identified from this tissue analysis and further verified with the pancreatic juice samples. From the tumor-normal paired tissue analysis of the expression levels of 2792 miRNAs, 20 upregulated and 17 downregulated miRNAs were identified. In IPMN associated with invasive carcinoma (INV), miR-10a-5p and miR-221-3p were upregulated and miR-148a-3p was downregulated when compared with noninvasive IPMN. When these findings were further validated with pancreatic juice samples, miR-10a-5p was found to be elevated in INV (p = 0.002). Therefore, three differentially expressed miRNAs were identified in tissues with INV, and the expression of miR-10a-5p was also elevated in pancreatic juice samples with INV. MiR-10a-5p is a promising additional biomarker for invasive IPMN.     

Perceived interactivity leading to e-loyalty: Development of a model for cognitive–affective user responses

Novel applications of website interactivity are important to attract and retain online users. In this empirical study five designs for interactivity are examined using different web-poll interfaces. The goal of the investigation is to examine perceived interactivity in a model which includes most commonly tested cognitive elements such as efficiency and effectiveness, but augments this model with the inclusion of a cognitive–affective element for trust, and an affective element of enjoyment. More specifically, a model is created to validate the relationship of perceived interactivity (comprised of user control, user connectedness, and responsiveness of the web-poll application) to efficiency, effectiveness, trust and enjoyment, of the website. In turn, efficiency, effectiveness, trust, and enjoyment are tested for their influence on user behavioral intentions for e-loyalty. All relationships in the model are supported. In addition, exploratory evaluation of qualitative comments is conducted to investigate additional insights between the five web-poll treatments in this investigation. The research confirms the complexity of a model in which cognitive, cognitive–affective and affective elements are present, and advances knowledge on the consequences of perceived interactivity. In additional to theoretical advancements, the research has merit for web designers and online marketers regarding how to enhance interactive online web applications.     

Mineral admixtures in mortars: Effect of inert materials on short-term hydration

This work is the first part of an overall project the aim of which is the development of general mix design rules for concrete containing different kinds of mineral admixtures. The separation of the different physical effects responsible for the modification of cement hydration, when chemically inert quartz powders are used in mortars, is presented. The phenomenological approach, based on semi-adiabatic calorimetry, is only associated with first-order phenomena, and the study excludes the complex physicochemical details involved in the chemistry of cement. The results, obtained for a wide range of fineness (between 180 and 2000 m²/kg) and replacement rates (up to 75%), show that short-term degrees of hydration in mortars containing a chemically inert mineral admixture (quartz) are always higher than for a reference mortar. This study confirms that cement hydration is enhanced by inert mineral admixtures. The two main physical effects responsible for the modification of the hydration of cement are identified as the dilution effect and heterogeneous nucleation. The dilution effect of the cement is highlighted with a coarse mineral admixture. The heterogeneous nucleation effect, although it increases with fineness of mineral admixtures, presents an optimum depending on the replacement rate. In the following part of this work, these results will be used for the development of an empirical model allowing us to quantify both physical effects.     

L'échelle de dramatisation face à la douleur PCS-CF: Adaptation canadienne en langue fran?aise de l'échelle Pain Catastrophizing Scale.

This study examined the psychometric properties of the PCS-CF, a French-Canadian adaptation of the Pain Catastrophizing Scale (Sullivan, Bishop & Pivik, 1995). One hundred and twenty undergraduate students (83 women; 37 men) completed the PCS-CF and measures of anxiety and depression prior to immersing their hand in ice water for 1-minute. A subset of 39 participants (28 females; 11 males) also completed a second pain task involving an ascending series of electrocutaneous stimulations. Participants' verbal and non-verbal pain behaviours during and immediately after the immersion task were recorded and subsequently independently coded. Reliability analyses revealed that the PCS-CF total score and subscale scores have a high degree of internal consistency and test-retest reliabilities that are comparable to the original Pain Catastrophizing Scale. PCS-CF scores were associated with higher levels of self-reported pain during the ice water immersion task and decreased pain tolerance during electrocutaneous stimulation. Individuals with elevated PCS-CF scores also displayed a more diverse repertoire of pain behaviours than their low PCS-CF counterparts. Previous findings that catastrophizing scores are able to discriminate criterion groups of males and females were also replicated. Given the highly correlated factors obtained in analyses of the dimensionality of the PCS-CF, the structure suggested by the authors of the original scale is only partially supported. However, the results taken together suggest that the PCS-CF is both a reliable and valid measure of the pain catastrophizing construct that is psychometrically comparable to the original PCS. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

NMR-Guided Repositioning of Non-Steroidal Anti-Inflammatory Drugs into Tight Junction Modulators

Bioavailability is a major bottleneck in the clinical application of medium molecular weight therapeutics, including protein and peptide drugs. Paracellular transport of these molecules is hampered by intercellular tight junction (TJ) complexes. Therefore, safe chemical regulators for TJ loosening are desired. Here, we showed a potential application of select non-steroidal anti-inflammatory drugs (NSAIDs) as TJ modulators. Based on our previous observation that diclofenac and flufenamic acid directly bound various PDZ domains with a broad specificity, we applied solution nuclear magnetic resonance techniques to examine the interaction of other NSAIDs and the first PDZ domain (PDZ1) of zonula occludens (ZO)-1, ZO-1(PDZ1). Inhibition of ZO-1(PDZ1) is expected to provide loosening of the epithelial barrier function because the domain plays a crucial role in maintaining TJ integrity. Accordingly, diclofenac and indomethacin were found to decrease the subcellular localization of claudin (CLD)-2 but not occludin and ZO-1 at the apicolateral intercellular compartment of Madin–Darby canine kidney (MDCK) II cells. These NSAIDs exhibited 125–155% improved paracellular efflux of fluorescein isothiocyanate insulin for the Caco-2 cell monolayer. We propose that these NSAIDs can be repurposed as drug absorption enhancers for peptide drugs.     

Maresin-1 and Inflammatory Disease

Inflammation is an essential action to protect the host human body from external, harmful antigens and microorganisms. However, an excessive inflammation reaction sometimes exceeds tissue damage and can disrupt organ functions. Therefore, anti-inflammatory action and resolution mechanisms need to be clarified. Dietary foods are an essential daily lifestyle that influences various human physiological processes and pathological conditions. Especially, omega-3 fatty acids in the diet ameliorate chronic inflammatory skin diseases. Recent studies have identified that omega-3 fatty acid derivatives, such as the resolvin series, showed strong anti-inflammatory actions in various inflammatory diseases. Maresin-1 is a derivative of one of the representative omega-3 fatty acids, i.e., docosahexaenoic acid (DHA), and has shown beneficial action in inflammatory disease models. In this review, we summarize the detailed actions of maresin-1 in immune cells and inflammatory diseases.