An annual assessment of air quality with the CALIOPE modeling system over Spain

The CALIOPE project, funded by the Spanish Ministry of the Environment, aims at establishing an air quality forecasting system for Spain. With this goal, CALIOPE modeling system was developed and applied with high resolution (4 km × 4 km, 1 h) using the HERMES emission model (including emissions of resuspended particles from paved roads) specifically built up for Spain. The present study provides an evaluation and the assessment of the modeling system, coupling WRF-ARW/HERMES/CMAQ/BSC-DREAM8b for a full-year simulation in 2004 over Spain. The evaluation focuses on the capability of the model to reproduce the temporal and spatial distribution of gas phase species (NO₂, O₃, and SO₂) and particulate matter (PM10) against ground-based measurements from the Spanish air quality monitoring network. The evaluation of the modeling results on an hourly basis shows a strong dependency of the performance of the model on the type of environment (urban, suburban and rural) and the dominant emission sources (traffic, industrial, and background). The O₃ chemistry is best represented in summer, when mean hourly variability and high peaks are generally well reproduced. The mean normalized error and bias meet the recommendations proposed by the United States Environmental Protection Agency (US-EPA) and the European regulations. Modeled O₃ shows higher performance for urban than for rural stations, especially at traffic stations in large cities, since stations influenced by traffic emissions (i.e., high-NO_x environments) are better characterized with a more pronounced daily variability. NO_x/O₃ chemistry is better represented under non-limited-NO₂ regimes. SO₂ is mainly produced from isolated point sources (power generation and transformation industries) which generate large plumes of high SO₂ concentration affecting the air quality on a local to national scale where the meteorological pattern is crucial. The contribution of mineral dust from the Sahara desert through the BSC-DREAM8b model helps to satisfactorily reproduce episodic high PM10 concentration peaks at background stations. The model assessment indicates that one of the main air quality-related problems in Spain is the high level of O₃. A quarter of the Iberian Peninsula shows more than 30 days exceeding the value 120 μg m⁻³ for the maximum 8-h O₃ concentration as a consequence of the transport of O₃ precursors downwind to/from the Madrid and Barcelona metropolitan areas, and industrial areas and cities in the Mediterranean coast.     

Wnt/β-Catenin Signaling Contributes to Paclitaxel Resistance in Bladder Cancer Cells with Cancer Stem Cell-Like Properties

The Wnt/β-catenin pathway plays an important role in tumor progression and chemotherapy resistance and seems to be essential for the maintenance of cancer stem cells (CSC) in several tumor types. However, the interplay of these factors has not been fully addressed in bladder cancer. Here, our goal was to analyze the role of the Wnt/β-catenin pathway in paclitaxel resistance and to study the therapeutic efficacy of its inhibition in bladder cancer cells, as well as to determine its influence in the maintenance of the CSC-like phenotype in bladder cancer. Our results show that paclitaxel-resistant HT1197 cells have hyperactivation of the Wnt/β-catenin pathway and increased CSC-like properties compared with paclitaxel-sensitive 5637 cells. Paclitaxel sensitivity diminishes in 5637 cells after β-catenin overexpression or when they are grown as tumorspheres, enriched for the CSC-like phenotype. Additionally, downregulation of β-catenin or inhibition with XAV939 sensitizes HT1197 cells to paclitaxel. Moreover, a subset of muscle-invasive bladder carcinomas shows aberrant expression of β-catenin that associates with positive expression of the CSC marker ALDH1A1. In conclusion, we demonstrate that Wnt/β-catenin signaling contributes to paclitaxel resistance in bladder cancer cells with CSC-like properties.     

Desulfurization of Middle Distillates by Oxidation and Extraction Process

In this work, we studied the desulfurization of Straight Run Gas Oil (SRGO) and diesel by selective oxidation and extraction of sulfur-containing compounds. The oxidation was carried out with a mixture of hydrogen peroxide at 30 wt% and acetic acid as catalyst. The extraction of the corresponding sulfones was performed with polar solvents (e.g., acetonitrile, methanol, 2-ethoxyethanol, and γ-butyrolactone). The SRGO has a sulfur content of 13,985 ppmw while diesel has 390 ppmw, the results showed sulfur removal to levels as low as 92 ppmw for diesel. From a consideration of the above results, a preliminary estimation of investment for a desulfuration process for hydrotreated diesel was done.     

The use of a modelling system as a tool for air quality management: annual high-resolution simulations and evaluation

The high levels of air pollutants over the North-Western Mediterranean (NWM) exceed the thresholds set in current air quality regulations. They demand a detailed diagnosis of those areas where the exceedances of thresholds related to human health are found. In this sense, there is a need for modelling studies for the specific area of the NWM that take into account the annual cycle to address the diagnosis of air pollution. A new approach to the modelling of air quality in the NWM has been adopted by combining the WRF-EMICAT-CMAQ-DREAM modelling system to diagnose the current status of the levels of photochemical air pollution (focusing on ozone, O(3); nitrogen dioxide, NO(2); carbon monoxide, CO; and particulate matter, PM10) in the area during an annual cycle (year 2004). The complexity of the area of study requires the application of high spatial and temporal resolution (2 km and 1 h). The annual simulations need to cover the complex different meteorological situations and types of episodes of air pollution in the area of study. The outputs of the modelling system are evaluated against observations from 52 meteorological and 59 air quality stations belonging to the Environmental Department of the Catalonia Government (Spain), which involve a dense and accurate spatial distribution of stations in the territory (32,215 km(2)). The results indicate a good behaviour of the model in both coastal and inland areas of the NWM, with a slight trend to the overestimation of tropospheric O(3) concentrations and the underestimation of other photochemical pollutants (NO(2), CO and PM10). The modelling diagnosis indicates that the main air quality-related problems in the NWM are the exceedances of the 1-hr O(3) information threshold set in the Directive 2002/3/EC (180 microg m(-3)) as a consequence of the transport of O(3) precursors downwind the Barcelona Greater Area (BGA); and the exceedances of the annual value for the protection of human health for NO(2) and PM10 (40 microg m(-3), Directive 1999/30/EC), both in the BGA, as a consequence of the high traffic-related emissions.