犬重症急性胰腺炎胰和肝组织的超微结构观察

重症急性胰腺炎 (ｓｅｖｅｒｅａｃｕｔｅｐａｎｃｒｅａｔｉｔｉｓ,ＳＡＰ)发病急、临床表现凶险、死亡率高 ,是当前外科棘手的问题。ＳＡＰ发病机理复杂 ,特别是多器官功能障碍的发病机制尚未阐明 ,尤其在组织超微结构方面研究的更少。本实验诱导犬重症急性胰腺炎模型 ,观察其胰、肝超微结构的改变 ,分析这些改变的原因、特点 ,为临床预防、诊治ＳＡＰ提供理论依据。材料与方法选用内蒙古医学院动物室提供的杂种犬 30只 ,体重 1 0～ 1 3ｋｇ ,不分雌雄 ,随机分为假手术组(1 5只 )和ＳＡＰ模型组 (1 5只 )。实验用所有动物术前禁食 2 4ｈ ,…     

Prognostic significance of interim ~(18)F-FDG PET/CT SUV reduction associated with Ki67 in patients with diffuse large B-cell lymphoma

To detect the prognostic significance of interim F-FDG PET/CT SUV(standard uptake value) reduction(△SUV_(max)) associated with Ki67 in patients with diffuse large B cell lymphoma(DLBCL).47 DLBCL patients underwent PET/CT before initiation and after 2-4 cycles of chemotherapy were included.The SUV_(max)of the dominant lesions were calculated.Ki67 positive indices were provided by enzyme-labeled immunohistochemistry.SPSS17.0 was used for statistical analysis.ASUV_(max) of different groups were compared by t test.Receiver-operator characteristic analysis was performed to determine the optimal cutoff values.KaplanMeier analyses of PFS(Progression-free survival) were compared using log-rank test.The average of △SUV_(max)and △SUV_(max)%were 11.53 and 69.10%,respectively.The optimal cutoff values of △SUV_(max)x and △SUV_(max)%were 11.45 and 82.92%,respectively.Higher △SUV_(max) and △SUV_(max)%indicated longer PFS(p 0.001).The optimal cutoff value of Ki67 was 55%.Ki6755%was revealed to be an indicator of shorter PFS(p = 0.019).Either △SUV_(max)≤11.45 or Ki6755%was defined as an indicator to poor outcome and scored 1 point.The PFS rate was 100%in patients scored 0 point,yet 0%in patients scored 2 points.PFS tended to be shorter along with the score getting higher(p = 0.006).△SUV_(max) and Ki67 positive index were both of significance in DLBCL prognosis.The prognostic value may be confirmed when △SUV_(max) was accordant with Ki67.     

131Ⅰ-BmK CT的制备及其在胶质瘤荷瘤大鼠体内分布与显像研究

为探索131I标记蝎氯毒素BmK CT的方法,研究标记物的稳定性、与细胞的结合能力及其在胶质瘤荷瘤Wistar大鼠体内的分布,通过克隆、表达、纯化得到多肽BmKCT,并采用Bolton-Hunter法间接标记BmKCT.胶质瘤荷瘤Wistar大鼠鼠尾静脉注射131I-BmK CT后于不同时间显像,并于不同时间处死,取血...