排序方式: 共有11条查询结果,搜索用时 22 毫秒
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新型基因枪微弹材料可行性初步研究-Ⅰ 总被引:1,自引:1,他引:0
基因枪在未来临床基因转导中很有前途,但目前所用微弹材料金、钨都属于重金属,其长期存在于人体的安全性尚无法估计。提出用羟基磷灰石(HAP)微粒来代替金或钨微粒,从理论上推导了这种取代的可行性,并报告了一种HAP微粒的制备及其负载DNA的研究结果。理论推导完全按照物理学方法进行。实验部分的方法:将稳定剂H以一定速度和比例滴入一定浓度的Ca(H2PO4)2溶液中,再按照一定的速度和比例滴入Ca(OH)2饱和溶液,期间或完成后超声处理一定时间,将所得HAP微粒溶液与亚精胺和DNA以一定比例和浓度在一定pH条件下混合。结论:所得HAP粒径值符合要求,并且能够高效负载DNA。 相似文献
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孙恩杰 《武汉理工大学学报(材料科学英文版)》1997,(4)
1 IntrodutionInterleukin-2(IL-2)was found to selectively keep growth of T lymphocytes for longperiod in vitro in 1976,and was then named T cell growth factor,TCGF.After that,IL-2 was found to promote proliferation of various cells,mainly including T,B,NK,and to in-crease activity of T cell and NK cell.Discovery of its surprising effect in treatment of can-cer,tumor through inducing LAK(lymphokine-activated killer cells)or activating TIL(tumor infiltrated lymphocytes)to kill cancer cell made it very attractive.Therefore it wasfound a wide application in therapy of cancer,immunodeficiency and diseases relating toinfection.However,in its application,problem was found that it had very serious side-effect,and very high dose made the side effect even more serious.The aim of this study was to find a simple way to stabilize IL-2 so as to lower the doserequired in application and in turn to solve the problem. 相似文献
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一种新型的组织工程支架材料-蚕丝 总被引:6,自引:0,他引:6
组织工程学是近来发展起来的一门新的学科.作为其主要部分,支架材料的选择应该满足特定的要求。蚕丝具有生物相容性、优异力学性能、较慢的降解速度.为组织工程支架的构建提供了新的选择。 相似文献
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新型聚羧酸系外加剂合成工艺研究 总被引:1,自引:0,他引:1
通过确定不同的引发剂用量、聚乙二醇投入量、聚氧化乙烯侧链长度以及酯化反应的温度和时间,对所合成的新型聚羧酸系外加剂的性能进行比较。试验结果表明,在新型聚羧酸系外加剂的合成过程中,存在着最佳范围的引发剂用量、聚乙二醇投入量、聚氧化乙烯侧链长度和最佳的酯化反应温度和时间,优化了新型聚羧酸外加剂的合成工艺。 相似文献
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基因枪在未来临床基因转导中很有前途,但目前所用微弹材料金、钨都属于重金属,其长期存在于人体的安全性尚无法估计,曾提出用羟基磷灰石(HAP)微粒来代替金或钨微粒。报告了两种HAP微粒的制备以及它们对DNA的负载及其影响因素研究的结果,以期对HAP在基因枪中的应用研究奠定基础。用化学沉淀法和热处理研磨法制备HAP微粒,调节pH负载DNA后,用红外光谱、同步热分析仪、透射电镜等方法表征负载。结果表明,可在一定条件下实现HAP对DNA的高效负载。 相似文献
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A high-efficiency capillary-fiber optic probe is developed for measuring the concentration of trace elements. The optimal probe consists of an excitation fiber (incident fiber) and a ring of collection fibers which is made up of 6 fibers. Both simulation and experiment results show that the structure gives the higher coupling efficiency with a reasonable capillary diameter. The coupling efficiency of the probe is determined by the number and arrangement of the fibers, internal diameter and length of the fiber optic sensing probe, and the end reflectivity of the capillary. The concentra-tion of the carbonic anhydrase solution and dezincification reagents also affect the efficiency. A fluorescence efficiency of 2.4% is obtained in zinc detection experiment. 相似文献