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城市空间形态研究的价值在于它在城市动态变化的过程中适当安排新的结构元素的能力,评价规划工作的优劣在某种意义上是指得到规划许可的城市发展的结果。因此,城市空间形态的研究对辅助与充实城市总体规划实践具有重要意义。低碳城市空间形态研究,可以对连片发展的城市形态、带形城市以及组团城市形态各自在减少碳排放方面进行评估,从而得出理想的城市空间形态。 相似文献
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The plasmid-expression system is routinely plagued by potential plasmid instability. Chromosomal in-tegration is one powerful approach to overcome the problem. Herein we report a plasmid-free hyper-producer E. coli strain for coenzyme Q10 production. A series of integration expression vectors, pxKC3T5b and pxKT5b, were constructed for chemically inducible chromosomal evolution (multiple copy integration) and replicon-free and markerless chromosomal integration (single copy integration), respectively. A coenzyme Q10 hyper-producer Es-cherichia coli TBW20134 was constructed by applying chemically inducible chromosomal evolution, replicon-free and markerless chromosomal integration as well as deletion of menaquinone biosynthetic pathway. The engineered E. coli TBW20134 produced 10.7 mg per gram of dry cell mass (DCM) of coenzyme Q10 when supplemented with 0.075 g·L^-1 of 4-hydroxy benzoic acid;this yield is unprecedented in E. coli and close to that of the commercial producer Agrobacterium tumefaciens. With this strain, the coenzyme Q10 production capacity was very stable after 30 sequential transfers and no antibiotics were required during the fermentation process. The strategy presented may be useful as a general approach for construction of stable production strains synthesizing natural products where various copy numbers for different genes are concerned. 相似文献
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数字水印的基本功能是将产权、产品的标识码以及购买者的信息等嵌入到数字媒体中。用小波变换的方法对数字音频的水印的嵌入和提取做了仿真研究,仿真结果表明该方法是可行的。 相似文献
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Escherichia coli BW25113 was metabolically engineered for CoQ10 production by replacing ispB with ddsA from Gluconobacter suboxydans.Effects of precursor balance and reduced nicotinamide-adenine dinucleotide phosphate (NADPH) availability on CoQ10 production in E.coli were investigated.The knockout of pykFA along with pck overexpression could maintain a balance between glyceraldehyde 3-phosphate and pyruvate,increasing CoQ10 production.Replacement of native NAD-dependent gapA with NADP-dependent gapC from Clostridium acetobutylicum,together with the overexpression of gapC,could increase NADPH availability and then enhanced CoQ10 production.Three effects,overexpressions of various genes in CoQ biosynthesis and central metabolism,different vectors and culture conditions on CoQ10 production in E.coli,were all investigated.The investigation of different vectors indicated that low copy number vector may be more beneficial for CoQ10 production in E.coli.The recombinant E.coli (△ispB::ddsA,△pykFA and △gapA::gapC),harboring the two plasmids encoding pck,dxs,idi and ubiCA genes under the control of PT5 on pQE30,ispA,ddsA from Gluconobacter suboxydans and gapC from Clostridium acetobutylicum under the control of PBAD on pBAD33,could produce CoQ10 up to 3.24 mg·g-1 dry cell mass simply by changing medium from M9YG to SOB with phosphate salt and initial culture pH from 7.0 to 5.5.The yield is unprecedented and 1.33 times of the highest production so far in E.coli. 相似文献
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Escherichia coli BW25113 was metabolically engineered for CoQ10 production by replacing ispB with ddsA from Gluconobacter suboxydans. Effects of precursor balance and reduced nicotinamide-adenine dinucleotide phosphate (NADPH) availability on CoQ10 production in E. Coli were investigated. The knockout of pykFA along with pck overexpression could maintain a balance between glyceraldehyde 3-phosphate and pyruvate, increasingCoQ10 production. Replacement of native NAD-dependent gapA with NADP-dependent gapC from Clostridium acetobutylicum, together with the overexpression of gapC, could increase NADPH availability and then enhanced CoQ10 production. Three effects, overcxpressions of various genes in CoQ biosynthesis and central metabolism,different vectors and culture conditions on CoQ10 production in E. Coli, were all investigated. The investigation of different vectors indicated that low copy number vector may be more beneficial for CoQ10 production in E. Coli.The recombinant E. Coli (AispB::ddsA, ApykFA and ΔgapA::gapC), harboring the two plasmids encoding pck, dxs,idi and ubiCA genes under the control of PT5 on pQE30, ispA, ddsA from Gluconobacter suboxydans and gapC from Clostridium acetobutylicum under the control of PBAD on pBAD33, could produce CoQ10 up to 3.24 mg.g-1 dry cell mass simply by changing medium from M9YG to SOB with phosphate salt and initial culture pH from 7.0 to 5.5. The yield is unprecedented and 1.33 times of the highest production so far in E. Coli. 相似文献