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排序方式: 共有82条查询结果,搜索用时 15 毫秒
1.
Molecular cloning of a novel melanocortin receptor 总被引:2,自引:0,他引:2
I Gantz Y Konda T Tashiro Y Shimoto H Miwa G Munzert SJ Watson J DelValle T Yamada 《Canadian Metallurgical Quarterly》1993,268(11):8246-8250
Using the technique of the polymerase chain reaction primed with oligonucleotides based on the homologous transmembrane regions of seven transmembrane G protein-linked receptors, we isolated three full-length human genes that encode a novel subgroup of this receptor family. Recently, two of these receptors were identified as specific for alpha-melanocyte-stimulating hormone (alpha-MSH) and adrenocorticotropic hormone. We report the molecular cloning and pharmacologic characterization of a third member of this subgroup. The gene for this receptor encodes a protein of 361 amino acids in length. Its pharmacology characterizes it as an MSH receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. By Northern blot hybridization and polymerase chain reaction, it is expressed in brain, placental, and gut tissues but not in melanoma cells or in the adrenal gland. These findings may yield insight into the physiology of peptides derived from pro-opiomelanocortin post-translational processing. 相似文献
2.
Optical scattering in beef steak to predict tenderness using hyperspectral imaging in the VIS-NIR region 总被引:1,自引:0,他引:1
Kim Cluff Govindarajan Konda Naganathan Jeyamkondan Subbiah Renfu Lu Chris R. Calkins Ashok Samal 《Sensing and Instrumentation for Food Quality and Safety》2008,2(3):189-196
The objective of this research is to develop a non-destructive method for predicting cooked beef tenderness using optical
scattering of light on fresh beef muscle tissue. A hyperspectral imaging system (λ = 496–1,036 nm) that consists of a CCD
camera and an imaging spectrograph, was used to acquire beef steak images. The hyperspectral image consisted of 120 bands
with spectral intervals of 4.54 nm. Sixty-one fresh beef steaks, including 44 strip loin and 17 tenderloin cuts, were collected.
After imaging, the steaks were cooked and Warner-Bratzler shear (WBS) force values were collected as tenderness references.
The optical scattering profiles were derived from the hyperspectral images and fitted to the modified Lorentzian function.
Parameters, such as the peak height, full scattering width at half maximum (FWHM), and the slope around the FWHM were determined
at each wavelength. Stepwise regression was used to identify 7 key wavelengths and parameters. The parameters were then used
to predict the WBS scores. The model was able to predict WBS scores with an R = 0.67. Optical scattering implemented with hyperspectral imaging shows limited success for predicting current status of tenderness
in beef steak. 相似文献
3.
Yoshiyuki Sano Marcekazu Konda Chan Woo Lee Yoshiharu Kimura Takeo Saegusa 《大分子材料与工程》1997,251(1):181-191
A new technique for imparting antistatic properties to poly(ethylene terephthalate) (PET) fiber has been developed. In this technique, blend polyester fibers containing poly(ethylene terephthalate/5-sulfoisophthalate) (SIP-PET) were prepared by blend spinning and then treated with various cationic surfactants in the process of dyeing. The surfactants could effectively be immobilized on the fiber as the counter cations of the sulfonate groups of the 5-sulfoisophthalate (SIP) units and aid the release of static electrons formed in the fiber. Thus, the half-life time (t1/2) of leakage of static charge and the surface resistivity (Rs) of the blend PET fibers became much lower after treating. The best result was obtained with a methylated quaternary ammonium salt of a stearylamine-ethylene oxide (EO) adduct or hydrochloride of a laurylamine-EO adduct as the surfactant of which the number of EO units was around ten. Even after five washing cycles the t1/2 value of the fibers treated with these surfactants was kept lower than 30 s with the Rs value maintained in the order of 1013 Ω cm-2. Therefore, the present technique could be useful for practical production of polyester fibers with “semi-permanent” antistatic properties which can be recovered by re-treatment even if they were lost. 相似文献
4.
Umamaheswara Konda Tarunraj Singh Puneet Singla Peter Scott 《Environmental Modelling & Software》2010,25(12):1608-1618
Atmospheric dispersion is a complex nonlinear physical process with numerous uncertainties in model parameters, inputs, source parameters, initial and boundary conditions. Accurate propagation of these uncertainties through the dispersion models is crucial for a reliable prediction of the probability distribution of the states and assessment of risk. A simple three-dimensional Gaussian puff-based dispersion model is used as a test case to study the effect of uncertainties in the model parameters and initial conditions on the output concentration. A polynomial chaos based approach is used to numerically investigate the evolution of the model output uncertainties due to initial condition and parametric uncertainties. The polynomial chaos solution is found to be an accurate approximation to ground truth, established by Monte Carlo simulation, while offering an efficient computational approach for large nonlinear systems with a relatively small number of uncertainties. 相似文献
5.
We study a linear stochastic approximation algorithm that arises in the context of reinforcement learning. The algorithm employs a decreasing step-size, and is driven by Markov noise with time-varying statistics. We show that under suitable conditions, the algorithm can track the changes in the statistics of the Markov noise, as long as these changes are slower than the rate at which the step-size of the algorithm goes to zero. 相似文献
6.
Our objective was to assess the effects of lactate enhancement in combination with different packaging systems on beef longissimus lumborum and psoas major steak color. Strip loins and tenderloins (n = 16) were assigned to one of four injection treatments (non-injected control, water-injected control, 1.25%, and 2.5% lactate in the finished product). Steaks were individually packaged in either vacuum, high-oxygen (80% O2/20% CO2), or 0.4% CO (30% CO2/69.6% N2) and stored for either 0, 5, or 9 days at 1 °C. The L∗ and a∗ values of both the longissimus and psoas responded similarly to lactate, which at 2.5% darkened steaks (P < 0.05) packaged in all atmospheres and improved (P < 0.05) the redness of steaks packaged in high-oxygen. Packaging steaks in CO did not counteract the darkening effects of lactate. Nevertheless, CO improved (P < 0.05) color stability compared with high-oxygen packaging. 相似文献
7.
Govindarajan Konda Naganathan Lauren M. Grimes Jeyamkondan Subbiah Chris R. Calkins Ashok Samal George E. Meyer 《Sensing and Instrumentation for Food Quality and Safety》2008,2(3):178-188
Tenderness is a primary determinant of consumer satisfaction of beef steaks. The objective of this study was to implement
and test near-infrared (NIR) hyperspectral imaging to forecast 14-day aged, cooked beef tenderness from the hyperspectral
images of fresh ribeye steaks (n = 319) acquired at 3–5 day post-mortem. A pushbroom hyperspectral imaging system (wavelength range: 900–1700 nm) with a diffuse-flood
lighting system was developed. After imaging, steaks were vacuum-packaged and aged until 14 days postmortem. After aging,
the samples were cooked and slice shear force (SSF) values were collected as a tenderness reference. After reflectance calibration,
a Region-of-Interest (ROI) of 150 × 300 pixels at the center of longissimus muscle was selected. Partial least squares regression (PLSR) was carried out on each ROI image to reduce the dimension along
the spectral axis. Gray-level textural co-occurrence matrix analysis with two quantization levels (64 and 256) was conducted
on the PLSR bands to extract second-order statistical textural features. These features were then used in a canonical discriminant
model to predict three beef tenderness categories, namely tender (SSF ≤ 205.80 N), intermediate (205.80 N < SSF < 254.80 N),
and tough (SSF ≥ 254.80 N). The model with a quantization level of 256 performed better than the one with a quantization level
of 64. This model correctly classified 242 out of 314 samples with an overall accuracy of 77.0%. Fat, protein, and water absorption
bands were identified between 900 and 1700 nm. Our results show that NIR hyperspectral imaging holds promise as an instrument
for forecasting beef tenderness. 相似文献
8.
9.
10.
This paper presents a methodology for the estimation of temperature dependent heat transfer coefficient for a vertical rectangular fin by using the inverse heat transfer method with Liquid crystal thermography (LCT) data. Steady state, laminar natural convection experiments have been done on a vertical rectangular fin of size 150 × 250 × 4, (L × w × t, all dimensions are in mm). The variation of heat transfer coefficient is considered as a power law function of temperature excess (h = aoθb) and is derived from the basic Nusselt number equation used for laminar natural convection, Nu = aRab. With this functional form, the one dimensional fin equation in finite difference form is repeatedly solved using the Gauss–Seidel iterative method. Treating this as a one parameter estimation in ‘a’ the sum of the squares of the difference between the simulated and Thermochromic Liquid Crystal (TLC) measured temperatures is minimized with the Golden section search algorithm to retrieve ‘a’. Estimate of ‘a’ and the accompanying uncertainties are first reported for synthetically generated temperature distribution for assumed values of ‘a’. The effect of noise on the estimate of ‘a’ is discussed. This is followed by retrievals with experimentally obtained TLC temperature distribution for a range of heat inputs to the fin base. The required temperature distributions for accomplishing the retrievals over the surface are obtained using calibrated R40C5W Thermochromic Liquid Crystal (TLC) sheets. As an additional proof of the accuracy of the method, the retrieved value of ‘a’ is used to simulate the temperature distribution in the fin which is then compared with the actual TLC measured temperature distribution. 相似文献