Structure-permeation relations of met-enkephalin peptide analogues on absorption and secretion mechanisms in Caco-2 monolayers

Due to the low effective permeabilities of peptides at many absorption sites, their structure-permeation relations are of high interest. In this work structure-permeation relations of Met-enkephalin analogues are presented using confluent Caco-2 cells as an in vitro permeation model. Four model peptides (Met-enkephalin, [D-Ala2]Met-enkephalin, [D-Ala2]Met-enkephalinamide, and metkephamid) were tested in terms of permeability, lipophilicity, charge, and molecular size. Permeability coefficients (P(eff)) across Caco-2 cells were low, 3.3 x 10(-8) to 9.5 x 10(-8) cm s-1, and were similar to typical paracellular markers. No correlation of permeability and the log(apparent octanol/buffer partition coefficient) was observed. A 40-fold increase of the permeability of metkephamid in the presence of 10 mM EDTA suggested a significant contribution of paracellular transport. Independent support for this conclusion was obtained by visualizing the pathway of the fluorescein isocyanate isomer I 1-metkephamid by confocal laser scanning microscopy (CLSM). The fluorophore-labeled peptide was observed in the intercallular space only. Metkephamid permeabilities were found to be direction-specific. Permeabilities from basolateral to apical (b-to-a) were significantly higher (ca. 4-fold) than in the opposite (a-to-b) direction. The addition of verapamil equalized the permeabilities in the a-to-b and b-to-a directions, suggesting the involvement of a P-glycoprotein-mediated secretion mechanism. Similar observations were obtained with [D-Ala2]Met-enkephalinamide, but not with Met-enkephalin and [D-Ala2]Met-enkephalin. In contrast to the other analogues, metkephamid and [D-Ala2]Met-enkephalinamide are positively charged at neutral pH, as demonstrated by their isoelectric points (pl = 8.6 for [D-Ala2]Met-enkephalinamide and metkephamid and 5.3 for [D-Ala2]Met-enkephalin and Met-enkephalin). The data is in agreement with the literature showing that most compounds secreted by the P-glycoprotein transporter carry a positive charge.     

Recent Development of Cognitive and Neuropsychological Assessment in Asian Countries.

There is a growing trend of research in neuropsychological assessment in Asia: The mean number of published studies increased from 1.8 in the 1980s to 16.0 in 2 recent years. About 40% of the studies reviewed concerned test development or adaptation, with the latter greatly outnumbering the former. To facilitate the test selection for clinical use, 36 clinical measures were evaluated based on some statistical criteria. The results showed that only 8 neuropsychological tests (5 Chinese. 2 Korean, and 1 Japanese) met the criteria. Given that Asian and Western populations differ on certain cognitive processes, future development of neuropsychological assessment measures in Asia should move from the adaptation of Western tests toward the construction of original tests with better ecological validity. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Modeling Temperature in Coupled Electrical and Thermal Simulations of the Electroconsolidation® Process

Electroconsolidation® is a process for densifying complex-shaped parts by using electrically conductive particulate solids as a pressure-transmitting medium. The part is immersed in a bed of the particulate medium contained in a die chamber. Sintering temperature is achieved by resistive heating of the medium while applying compaction pressure. The process is capable of ultrahigh temperatures and short cycle times and offers the potential for low processing costs.

Control of the process and selection of process conditions require knowledge of the temperatures within the die. Temperature gradients exist because of the high heating rate and because of variations of density and electrical resistivity of the medium due to the presence of the part. Direct measurement of temperature with thermocouples or other conventional means is impractical because of the high temperatures, high currents, and high pressures that are involved. Therefore, a computer model was developed to predict temperature as a function of time and applied voltage for any location in the die. The computer model is composed of three parts: a geometrical model to approximate the density and resistivity variations in the medium, a finite-element model to calculate the rate of resistive heating within each element, and a finite-difference model to calculate the temperature distribution based on solution of the heat-transfer equations. Predicted temperatures have been shown to be in excellent agreement with measurements, and numerical simulation provided encouraging consistency and reasonably accurate predictions of temperature profiles within the die. The model demonstrated the feasibility of a new process to achieve simultaneous application of pressure and heat to powder densification in Electroconsolidation.     

Relationships among lactate concentration, blood flow and histopathologic profiles in rat C6 glioma

Increased capacity for glycolytic metabolism is a well-known characteristic of neoplastic cells. Because lactic acid is the end product of glycolysis, in vivo MRS measurements of tumor lactate concentration ([lac]t) may provide valuable information about tumor metabolism, which will aid the development of therapies and the clinical diagnosis and treatment of tumors. In the present study, several hemodynamic and histologic parameters were evaluated with respect to their influence on [lac]t. Pronounced differences in [lac]t in two distinct populations of tumors suggested a putative perfusion threshold. Above this threshold, [lac]t was independent of hemodynamic and histologic factors including tumor blood flow (measured using MRS and the method of D2O washout), extent of necrosis and inflammatory cell infiltrate. Thus, for most tumors, [lac]t was not determined by any one single factor such as hypoxia, venous clearance, glucose supply, extent of necrosis or degree of inflammatory cell infiltrate. Rather, [lac]t may be equilibrated, at least in part, by an interplay of forces involving hemodynamics and substrate supply. In general, the data are consistent with the hypothesis that elevated lactate in most tumors is related to the high glycolytic activity of adequately perfused, viable neoplastic cells.     

A new environmental friendly method for the preparation of sugar acids via catalytic oxidation on gold catalysts

A novel and efficient way of producing of aldonic acids is reported. The selective catalytic oxidation of monosaccharides (arabinose, ribose, xylose, lyxose, mannose, rhamnose, glucose, galactose, N-acetyl-glucosamine) and disaccharides (lactose, maltose, cellobiose, melibiose) on Au, Pd and Pt catalysts was investigated. By using the gold catalyst, a total selectivity with respect to aldonic acids and a high catalytic activity for all investigated sugars was found. The reaction conditions for the production of maltobionic and lactobionic acids via oxidation on gold catalysts were optimised. A high long-term stability of two different gold catalysts for the oxidation of maltose and lactose, respectively, was found.     

An elementary proof of Sylvester’s double sums for subresultants

In 1853 Sylvester stated and proved an elegant formula that expresses the polynomial subresultants in terms of the roots of the input polynomials. Sylvester’s formula was also recently proved by Lascoux and Pragacz using multi-Schur functions and divided differences. In this paper, we provide an elementary proof that uses only basic properties of matrix multiplication and Vandermonde determinants.     

Towards a distributed multi-agent framework for shared resources scheduling

Nowadays, manufacturers have to share some of their resources with partners due to the competitive economic environment. The management of the availability periods of shared resources causes a problem because it is achieved by the scheduling systems, which assume a local environment where all resources are on the same site. Therefore, distributed scheduling with shared resources is an important research topic. In this communication, we introduce the architecture and behaviour of DSCEP framework (Distributed, Supervisor, Customer, Environment, and Producer) under shared resources situation with disturbances. We are using a simple example of manufacturing system to illustrate the ability of DSCEP framework to solve the shared resources scheduling problem in complex systems.     

Mechanical and dynamical mechanical properties of chloroprene rubber and cellulose II composites

Vulcanized composites of chloroprene rubber (CR) with cellulose II (Cel II) as a filler were investigated. Cel II, obtained by the coagulation of cellulose xanthate, was incorporated in the rubber by the traditional method. The filler content varied from 0 to 30 phr. For comparison purposes, carbon black (CB)–CR composites were also studied. The CB amount varied from 0 to 45 phr. The mechanical and dynamic mechanical properties were determined, and the CR composite containing 20 phr of Cel II showed the best set of properties. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 92: 2425–2430, 2004     

The significance of defect chemistry for the rate of gas–solid reactions: three examples

Three examples are revisited in which the reaction rate could be reliably correlated with point defect chemistry highlighting the role of point defects as acid–base active centers. In the case of dehydrohalogenation of tertiary butyl chloride, AgCl becomes increasingly active as heterogeneous catalyst, if AgCl is homogeneously or heterogeneously doped. By such a procedure the silver vacancy concentration is adequately increased. The oxygen incorporation into SrTiO₃ offers an example in which the surface mechanism in terms of adsorbed species, oxygen vacancies and electronic centers has been elucidated. Appropriate surface coatings give rise to significant catalytic effects. Increasing iron (acceptor) doping not only changes the point defect chemistry but also the nature of the rate determining step. Lastly, the electrocatalytic function of Sr-doped LaMnO₃ is considered as regards oxygen reduction reaction and O²⁻ incorporation into Y-doped ZrO₂ in the context of solid oxide fuel cells. Again the defect chemistry is of prime importance for the reaction rate.     

German Water Infrastructure in China: Colonial Qingdao 1898–1914

Within the colorful tapestry of colonial possessions the German empire acquired over the short period of its existence, Qingdao stands out because it fulfilled a different role from settlements in Africa—especially because of its exemplary planned water infrastructure: its technological model, the resulting (public) hygiene, and the adjunct brewery. The National Naval Office (Reichsmarineamt), which oversaw the administration of the future “harbour colony”—at first little more than a little fishing village—enjoyed a remarkable degree of freedom in implementing this project. The German government invested heavily in showing off its techno-cultural achievements to China and the world and thereby massively exploited the natural resources of the mountainous interior. This contribution focuses on Qingdao’s water infrastructure and its role in public hygiene and further area development. This article will not only use new empirical evidence to demonstrate that the water infrastructure was an ambivalent “tool of empire”. Relying on the concept of “urban metabolism,” this paper primarily traces the ecological consequences, particularly the landscape transformation of the mountains surrounding the bay and the implications for the region’s water resources. When evaluating colonial enterprises, changes in local ecology should play a significantly greater role.