Urine samples from C3H congenic house mice (
Mus domesticus) differing only at the
t complex were examined by capillary gas chromatography to assess variations in the volatile components that may cause olfactory discrimination between animals bearing
t lethal and+(wild-type) haplotypes. Urine was collected from 192 males and females varying in age from 1 to 9 months. C3H congenic mice that have the same genetic background at all loci but differed in their
t complex genotypes: +/ +, +/t
w1,
T/t
w1, T/+ were used. No urinary volatiles were unique to the
t complex. However, significant differences among
t complex genotypes and among ages occurred for concentrations of 12 male volatiles and four female volatiles. Usually young males (1–2 months of age) had significantly higher concentrations of cyclic enol ethers and ketones than older males (4–9 months of age). Moreover, some urinary volatiles (cyclic enol ethers, one ketone, dehydrobrevicomin, and thiazoline) were excreted in the urine of T/+and/or
T/t males in significantly higher concentration than in the urine of +/+ males. Age and
t complex genotype influences on the urinary volatiles in females were observed for four ketones. Gas chromatography of urinary components has the potential to be used in field studies of the
t complex because the two t complex genotypes found in wild populations, +/+ and +/t, had significant differences in concentration for two males volatiles and three female volatiles.We dedicate this paper to the memory of Dorothea Bennett.On leave from the Environmental Protection Institute, Ministry of Light Industry, Beijing, China.A.E.M.B. supported in part by NSF BSR 8909172.
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