全文获取类型
收费全文 | 4486篇 |
免费 | 12篇 |
国内免费 | 2篇 |
专业分类
电工技术 | 19篇 |
综合类 | 3篇 |
化学工业 | 235篇 |
金属工艺 | 25篇 |
机械仪表 | 39篇 |
建筑科学 | 60篇 |
矿业工程 | 10篇 |
能源动力 | 30篇 |
轻工业 | 131篇 |
水利工程 | 9篇 |
石油天然气 | 6篇 |
无线电 | 124篇 |
一般工业技术 | 210篇 |
冶金工业 | 3423篇 |
原子能技术 | 10篇 |
自动化技术 | 166篇 |
出版年
2021年 | 13篇 |
2020年 | 8篇 |
2019年 | 12篇 |
2018年 | 13篇 |
2016年 | 15篇 |
2015年 | 9篇 |
2014年 | 20篇 |
2013年 | 46篇 |
2012年 | 31篇 |
2011年 | 43篇 |
2010年 | 31篇 |
2009年 | 29篇 |
2008年 | 41篇 |
2007年 | 50篇 |
2006年 | 26篇 |
2005年 | 38篇 |
2004年 | 26篇 |
2003年 | 39篇 |
2002年 | 25篇 |
2001年 | 24篇 |
2000年 | 29篇 |
1999年 | 138篇 |
1998年 | 1077篇 |
1997年 | 642篇 |
1996年 | 376篇 |
1995年 | 236篇 |
1994年 | 192篇 |
1993年 | 207篇 |
1992年 | 36篇 |
1991年 | 62篇 |
1990年 | 46篇 |
1989年 | 54篇 |
1988年 | 73篇 |
1987年 | 61篇 |
1986年 | 42篇 |
1985年 | 54篇 |
1984年 | 17篇 |
1983年 | 20篇 |
1982年 | 32篇 |
1981年 | 28篇 |
1980年 | 42篇 |
1979年 | 20篇 |
1978年 | 36篇 |
1977年 | 91篇 |
1976年 | 188篇 |
1975年 | 16篇 |
1974年 | 18篇 |
1973年 | 13篇 |
1972年 | 15篇 |
1944年 | 9篇 |
排序方式: 共有4500条查询结果,搜索用时 31 毫秒
1.
2.
Phospholamban (PLN) was expressed in Escherichia coli as a protein fusion with glutathione S-transferase (GST). GST-PLN was mostly present in the insoluble protein fraction and accounted for approximately 50% of total insoluble protein. Attempts to suppress inclusion body formation or to use GST as an affinity-purification tag failed. A successful purification method is based on preparative SDS/PAGE and electrodialysis. From 1 g cells we typically purified 13.5 mg fusion protein with a PLN content of 2.8 mg. We genetically inserted an enterokinase (EK) protease site just in front of the PLN sequence and demonstrated the proteolytical liberation of PLN from the carrier protein. The approach described represents a substantial advancement in PLN expression and purification. 相似文献
3.
Math1 is essential for genesis of cerebellar granule neurons 总被引:1,自引:0,他引:1
N Ben-Arie HJ Bellen DL Armstrong AE McCall PR Gordadze Q Guo MM Matzuk HY Zoghbi 《Canadian Metallurgical Quarterly》1997,390(6656):169-172
4.
JM Piepmeier DL Keefe MA Weinstein D Yoshida J Zielinski TT Lin Z Chen F Naftolin 《Canadian Metallurgical Quarterly》1993,32(3):422-30; discussion 430-1
Estramustine is an estradiol-based agent that has been shown to accumulate in human glioma cells, resulting in a concentration-dependent alteration in cell size and shape within minutes and an inhibition of proliferation over 3 to 6 days. We evaluated human glioblastoma cultures with [3H]thymidine incorporation assays to determine estramustine's early effects on deoxyribonucleic acid synthesis in these tumors. Because estramustine shares a common structural motif with other antimicrotubule drugs, we synthesized four A-ring conjugates of estrone that contained a carbamate moiety but lacked nitrogen mustard. These analogs were examined by [3H]thymidine incorporation and compared with vinblastine. Greater than 70% inhibition of [3H]thymidine incorporation occurred within 1 hour of treatment with estramustine at 10(-5) mol/L, which increased to 80% inhibition at 4 hours. Ethyl carbamate JE208 was nearly as effective as estramustine in inhibiting deoxyribonucleic acid synthesis, and both were more effective than vinblastine. The inhibitory effects of estramustine and estrone analogs were reversible; vinblastine was not reversible. Although estramustine and JE208 induced similar antiproliferative and morphological changes in glioblastoma cells that persisted for at least 4 days, there was a modest recovery of morphology and thymidine incorporation with JE208 after prolonged treatment. The common findings with estramustine and JE208 suggest that these agents may have a similar mechanism of action and form the basis for the investigation of new agents that may rapidly and reversibly inhibit glioblastoma. 相似文献
5.
6.
S Hakki SJ Harwood MA Morrissey JG Camblin DL Laven WB Webster 《Canadian Metallurgical Quarterly》1997,(335):275-285
When clinical data are insufficient to diagnose infection of bone or joints, nuclear scanning becomes crucial in making an accurate diagnosis. The efficacy of (99m)technetium antigranulocyte monoclonal antibody Fab' fragment (LeukoScan) is prospectively compared with (111)indium white blood cell and (99m)technetium methylene diphosphonate bone scans in 74 patients with suspected musculoskeletal infections. They were grouped according to site of suspected infection: 33 long bones, 23 prosthetic joints, and 18 diabetic feet. Sixty-two of these 74 patients had surgical verification with histopathology or culture. The remaining 12 patients had clinical followup as proof of absence of infection. The overall sensitivity of LeukoScan, (111)indium white blood cell, and (99m)technetium methylene diphosphonate bone scans was 93%, 85% and 92%, respectively. Specificity was 89%, 75% and 52%, and accuracy was 90%, 79% and 74%, respectively. The conclusion from this study is that LeukoScan is more accurate in detecting osteomyelitis, with better sensitivity and specificity in prosthetic joints. Compared with (111)indium white blood cell scans, LeukoScan++ gives superior images, and results are obtained in 1 to 6 hours without biohazard risk from handling blood products. 相似文献
7.
Confocal immunofluorescence microscopy with anti-cytokeratin antibodies revealed a continuous and polarized network of cytokeratin (CK) filaments in the cortex of stage VI Xenopus oocytes. In the animal cortex, CK filaments formed a dense meshwork that both was thicker and exhibited a finer mesh than the network of CK filaments previously observed in the vegetal cortex (Klymkowsky et al., 1987). CK filaments first appeared in association with germinal vesicle (GV) and mitochondrial mass (MM) of oocytes in early mid stage I, indicating that CK filaments are the last of the three cytoskeletal networks to be assembled. By late stage I, CK filaments formed complex networks surrounding the GV, surrounding and penetrating the MM, and linking these networks to a meshwork of CK filaments in the oocyte cortex. During stage III-early IV, CK filaments formed a highly interconnected, apparently unpolarized, radial array linking the perinuclear and cortical CK filament networks. Polarization of the CK filament network was observed during mid stage IV-stage V, as first the animal, then the vegetal CK filament networks adopted the organization characteristic of stage VI oocytes. Treatment of stage VI oocytes with cytochalasin B disrupted the organization of both cortical and cytoplasmic CK filaments, releasing CK filaments from the oocyte cortex and inducing formation of numerous cytoplasmic CK filament aggregates. CB also disrupted the organization of cytoplasmic microtubules (MTs) in stage VI oocytes. Disassembly of oocyte MTs with nocodazole resulted in loss of the characteristic A-V polarity of the cortical CK filament network. In contrast, disruption of cytoplasmic CK filaments by microinjection of anti-CK antibodies had no apparent effect on cytoplasmic or MT organization. We propose a model in which the organization and polarization of the cortical network of CK filaments in stage VI Xenopus oocytes are dependent upon a hierarchy of interactions with actin filaments and microtubules. 相似文献
8.
DJ Conway MJ Holland RL Bailey AE Campbell OS Mahdi R Jennings E Mbena DC Mabey 《Canadian Metallurgical Quarterly》1997,65(3):1003-1006
Tumor necrosis factor alpha (TNF-alpha) may play a central role in the disease pathogenesis which occurs as a consequence of chlamydial infection. To investigate the importance of TNF-alpha gene promoter polymorphisms and TNF-alpha levels in tear fluid in scarring trachoma, a large matched-pair case-control study was performed in The Gambia. The -308A allele was present in a higher proportion of patients (28.4%) than controls (18.4%), with an increasing association for homozygotes (chi2 for trend, P = 0.032; allele frequency, 0.163 in patients and 0.099 in controls; chi2, P = 0.025). For the -238A allele, the association was similar but not significant. The disease association was highly significant when the number of either -308A or -238A sites in an individual was considered (P = 0.003). TNF-alpha promoter alleles are tightly linked to some HLA class I and II alleles, but multivariate analysis confirmed that the disease associations were independent of HLA, although a class I allele, A*6802, is also associated with disease. TNF-alpha was more frequently detected in tear samples from patients (27.6%) than from controls (15.9%), increasingly so for higher levels of detectable TNF-alpha (P = 0.015). Among patients, detectable TNF-alpha in tears was highly associated with the presence of ocular chlamydial infection (P < 0.001). The results indicate that TNF-alpha plays a major role in the tissue damage and scarring which occurs as a consequence of Chlamydia trachomatis infection. 相似文献
9.
This paper addresses the existence of loop gain-phase shaping (LGPS) solutions for the design of robust digital control systems for SISO, minimum-phase, continuous-time processes with parametric uncertainty. We develop the frequency response properties of LGPS for discrete-time systems using the Δ-transform, a transform method that applies to both continuous-time and discrete-time systems. A theorem is presented which demonstrates that for reasonable specifications there always exists a sampling period such that the robust digital control problem has a solution. Finally, we offer a procedure for estimating the maximum feasible sampling period for LGPS solutions to robust digital control problems. 相似文献
10.
DL Scott 《Canadian Metallurgical Quarterly》1993,20(3):582-584
Changes in clinical and laboratory measures of disease activity were studied prospectively in 12 European centers. Altogether 282 rheumatoid patients were evaluated during 6 months of therapy with slow-acting drugs. Patients' global assessment was taken to indicate overall response. The number of swollen joints and number of tender joints correlated highly with this. The erythrocyte sedimentation rate (ESR) correlated less well but was more uniform across centers. Grip strength, C-reactive protein and hemoglobin performed poorly between centers. There were cultural and linguistic difficulties using the Health Assessment Questionnaire in a European setting. Physician's global assessments were similar to the patient's global assessments and provided redundant information. The best measures are: the number of swollen joints, the number of tender joints, the ESR, and the patient's global assessment. It may also help to measure articular pain. 相似文献