Neuronal differentiation modifies the effect of ethanol exposure on voltage-dependent calcium channels in NG 108-15 cells

The effect of prolonged (72 h) ethanol (200 mM) exposure on the labeling of L-type (using tritiated PN 200-110) and N-type (using iodinated omega-conotoxin) voltage-dependent calcium channels was investigated in cultured NG 108-15 cells. In undifferentiated cells ethanol produced an 80% increase in PN 200-110 Bmax and no changes in omega-conotoxin binding. Differentiation had a profound effect on the response of cells to ethanol, which in differentiated neuron-like cells decreased omega-conotoxin binding (-53.5%) leaving PN 200-110 labeling of L-type channels unaffected. The effect was time dependent and reversible upon ethanol withdrawal. The decreased omega-conotoxin binding was accompanied by a reduced ability of omega-conotoxin to inhibit K+ -stimulated calcium uptake. The results demonstrate that in cultured NG 108-15 cells ethanol differentially affects DHP and omega-conotoxin-sensitive, voltage-dependent calcium channels and that the effect is also modulated by differentiation of the cell to a neuronal phenotype.     

Performance and robustness of block constraint preconditioners in finite element coupled consolidation problems

Block constraint preconditioners are a most recent development for the iterative solution to large‐scale, often ill‐conditioned, coupled consolidation problems. A major limitation to their practical use, however, is the somewhat difficult selection of a number of user‐defined parameters (at least 4) in a more or less optimal way. The present paper investigates the robustness of three variants of the block constraint preconditioning in relation to the above parameters. A theoretical analysis of the eigenspectrum of the preconditioned matrix provides relatively simple bounds of the eigenvalues as a function of these parameters. A number of test problems used to validate the theoretical results show that both the mixed constraint preconditioner (MCP) combined with the symmetric quasi‐minimal residual (SQMR) solver and the MCP triangular variant (T‐MCP) combined with the bi‐conjugate gradient stabilized (Bi‐CGSTAB) are efficient and robust tools for the solution to difficult Finite Element‐discretized coupled consolidation problems. Moreover, the practical selection of the user‐defined parameters is relatively easy as a stable behavior is observed for a wide range of fill‐in degree values. The theoretical bounds on the eigenspectrum of the preconditioned matrix may help to suggest the most appropriate parameter combination. Copyright © 2009 John Wiley & Sons, Ltd.     

Estimating maize ground cover using spectral data from Aqua-MODIS in Córdoba,Argentina

Ground cover by foliage is a biophysical property of vegetation linked both to the interception of photosynthetically active radiation and to the crop transpiration rate. The spectral information provided by the Moderate Resolution Imaging Spectroradiometer on board the Aqua (Aqua-MODIS) satellite, which has a spatial resolution of 250 m, is an observation and monitoring resource that may be appropriate for estimating the ground cover of maize when plots exceed 40 ha. In this research, 10 maize plots were monitored in the central region of the province of Córdoba, Argentina, during the 2005–2006 growing season, obtaining photographic records of ground cover and soil moisture data. The normalized difference vegetation index (NDVI) of the Aqua-MODIS images showed a significant linear relationship with maize ground cover which, when the complete cycle is taken into account, is sufficient to explain 87% of the variability of ground cover, with an RMSE of 9%, a level of accuracy that increases when the crop is in the vegetative stage and the moisture conditions of the soil are less limiting. Other vegetation indices and linear mixed models were assessed. In addition to using data from the red and near-infrared channels, they incorporate information about soil conditions, but they showed no predictive advantages compared to the NDVI, resulting in simple models that explained between 77% and 87% of the variability of ground cover, with RMSE values of between 9% and 14%.     

Prognosis of myasthenia gravis: a retrospective study of 380 patients

The 9139 follow-up records of 438 myasthenia gravis (MG) patients were reviewed. Excluding those patients who were diagnosed 5 or more years after symptom onset (n = 37) and those who experienced only oculomotor symptoms throughout follow-up (n = 21), there were 380 patients. A survival analysis approach was used to assess the influence of prognostic factors on the following endpoints: (a) stable complete remission, (b) complete remission of at least 6 months and (c) pharmacological remission of at least 6 months. Early diagnosis was associated with a better prognosis with respect to all endpoints. Thymectomy also improved the prognosis but only for those patients without thymoma. Later MG onset was associated with a higher tendency to achieve pharmacological remission.     

Analytical bias dependent noise model for InP HEMT's

A practical device model for both high frequency small signal and noise behavior of InP-HEMT's depending on both gate and drain voltage has been developed. The model is based on the two-piece linear approximation using charge control and saturation velocity models. Combining large signal model and analytical expressions for the noise source parameter P, R, and C, an analytical bias-dependent noise model can be obtained. For implementation into high frequency simulation software, the exact calculated bias dependence was mathematically fitted by elementary functions. It could be shown that lowest noise is observed when the drain current for maximum gain is reduced to a third while the drain voltage is reduced to the start of the saturation region V_ds =0.6 V. Modeling scaling effects of the noise behavior shows that lowest noise is observed for a gate width of 1×40 μm. Multi-finger layouts are preferable for gate widths above 70 μm. Furthermore it is shown, that the optimum width of each finger decreases with the number of fingers     

Value of oncologic follow-up of patients operated for colorectal cancer. A prospective study of 1000 patients

OBJECTIVE: The purpose of our prospective study was to evaluate the contribution of follow-up in resected colorectal cancer. METHODS: One-thousand patients with colorectal adenocarcinoma who underwent radial resection from January 1975 to January 1990 were prospectively divided into two groups: the first group (n = 442, 42%) entered a 5-year follow-up protocol and the second group (n = 558, 56%) was free to make unscheduled visits in case of symptoms. RESULTS: Recurrence was found in 31.5% of the patients in the first group compared with 33% in the second group. Chorioembryonic antigen was the most accurate test detecting recurrence: 77% of the cases (97% for hepatic metastasis). Surgical resection of recurrent tumors was performed in 37% of the group 1 patients (curative resection in 15%) and in 9% (curative resection in 1.5%) of the group 2 patients (p < 0.001). 5-year survival after recurrence in group 1 was 11.5% versus 1% in group 2 (p < 0.01). CONCLUSION: These results emphasize the rational for a follow-up program in patients undergoing surgery for colorectal cancer.     

Mechanisms of the effect of lead on brain neurotransmission: a calcium mediated action

Data on the effect of chronic lead ingestion on brain neurochemistry in laboratory rodents show the involvement of several neurotransmitters including catecholamines. In the case of dopamine (DA), the action of lead is not uniform in all the DA-innervated areas. The reason for the regional susceptibility to the effect of lead is at present unknown, but points to the existence of specific neuronal mechanisms. Results obtained from animals exposed from birth to lead show that calcium channels are differentially modified in various brain areas. In particular, calcium antagonists display regional sensitivity both after in vivo lead treatment and after in vitro lead addition. These data support the concept that the area selective effects of the metal on neurotransmission may depend on the mechanisms controlling nerve terminal activity in different neuronal populations.     

Influence of cap-layer doping on ohmic contacts for InP based HEMT structures

Ohmic contacts to lattice matched InP based HEMT structures with both doped and undoped GaInAs cap-layers have been investigated. Contact resistances as low as 0.09 Ωmm were achieved using annealed Ni---Ge---Au ohmic contacts. It is reported that the contact resistance is independent of the doping of the thin (50–100 Å) cap-layer. This indicates that it is the bandgap and not the doping of the cap-layer, which determines the contact resistance to the HEMT structure. Furthermore it was shown, that the contact resistance is reduced for lower sheet resistances of the 2DEG.     

Carbon nanotube-based nanocarriers: the importance of keeping it clean

Nanotechnology-introduced materials have promising applications as nanocarriers for drugs, peptides, proteins and nucleic acids. Several studies showed that the geometry (shape and size) and chemical properties of nanoparticles affect the kinetics and pathways of cellular uptake and their intracellular trafficking and signaling. Accurate physico-chemical characterization of nanoparticles customarily precedes their use in cell biology and in vivo experiments. However, a fact that is easily overlooked is that nanomaterials decorated with organic matter or resuspended in aqueous buffers can be theoretically contaminated by fungal and bacterial microorganisms. While investigating the effects of extensively characterized PEGylated carbon nanotubes (PNTs) on T lymphocyte activation, we demonstrated bacterial contamination of PNTs, which correlated with low reproducibility and artifacts in cell signaling assays. Contamination and artifacts were easily eliminated by preparing the materials in sterile conditions. We propose that simple sterile preparation procedures should be adopted and sterility evaluation of nanoparticles should be customarily performed, prior to assessing nanoparticle intracellular internalization, trafficking and their effects on cells and entire organisms.     

Exploration of the effect of farm,breed, sex and animal on detailed mineral profile of beef and their latent explanatory factors

Minerals in meat vary with different extrinsic and intrinsic sources; therefore, we aimed to quantify different sources of variation in mineral profile of beef. Longissimus thoracis muscle (N.182) from young bulls and heifers was analysed using ICP-OES for 6 macrominerals, 5 essential microminerals and 9 environmental microminerals. Results of mixed models show that breed and sex have little effect on mineral profile. Major sources of variation, such as farm, individual animal within farm and side/sample within animal, differed markedly from mineral to mineral, affecting reproducibility coefficients. Multivariate factor analysis revealed that five latent factors explained 69% of the co-variance of 20 minerals. The first was a quantitative factor, followed by others based on Na-Fe-Cu, on K-B-Pb, on Fe-Mn and on Zn content, respectively. In conclusion, minerals’ major source of variation depends on complex relationships between the animal’s genetics, physiology, farm environment, management and feeding, whose understanding is favoured by latent factor analyses.