The use of liposomes in the modification of polycaprolactone fibers

Polycaprolactone is fully bioresorbable and biocompatible material. Liposomes containing nanocopper, nanosilver, and nanogold are known to have antifungal and antibacterial properties and to further aid in the synthesis of collagen and elastin in the skin. It is possible to combine the properties of polycaprolactone fibers and liposomes in new approaches to deliver active substances through cosmetics and medicines. The aim of the research was to examine the possibility of simple modification of PCL fibers with use of nanocopper, nanogold, and nanosilver incorporated liposomes. The size and the type of the liposomes were examined using optical microscopy and DLS techniques. The fibres modified with liposomes were investigated using SEM and FTIR techniques. Additionally the contact angle measurements were performed. The study shows an innovative method of modifying polycaprolactone nonwoven textiles. This combination of PCL fibers and liposomes allows easy and efficient preparation and delivery of active substances to a particular location. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 43299.     

Evolutional searching for circuit structures

A new method based on a genetic algorithm is presented to search for graphs representing structures of circuits with a given transfer function. Operational transconductance amplifier-capacitor filters consisting of lossy and lossless integrators have been taken as an example     

Biochemical,Structural Analysis,and Docking Studies of Spiropyrazoline Derivatives

In this study, we evaluated the antiproliferative potential, DNA damage, crystal structures, and docking calculation of two spiropyrazoline derivatives. The main focus of the research was to evaluate the antiproliferative potential of synthesized compounds towards eight cancer cell lines. Compound I demonstrated promising antiproliferative properties, especially toward the HL60 cell line, for which IC₅₀ was equal to 9.4 µM/L. The analysis of DNA damage by the comet assay showed that compound II caused DNA damage to tumor lineage cells to a greater extent than compound I. The level of damage to tumor cells of the HEC-1-A lineage was 23%. The determination of apoptotic and necrotic cell fractions by fluorescence microscopy indicated that cells treated with spiropyrazoline-based analogues were entering the early phase of programmed cell death. Compounds I and II depolarized the mitochondrial membranes of cancer cells. Furthermore, we performed simple docking calculations, which indicated that the obtained compounds are able to bind to the PARP1 active site, at least theoretically (the free energy of binding values for compound I and II were −9.7 and 8.7 kcal mol⁻¹, respectively). In silico studies of the influence of the studied compounds on PARP1 were confirmed in vitro with the use of eight cancer cell lines. The degradation of the PARP1 enzyme was observed, with compound I characterized by a higher protein degradation activity.