A spectral attentional mechanism tuned to object configurations

This paper describes an attentional mechanism based on the interpretation of spectral signatures for detecting regular object configurations in areas of an image delineated using context information. The proposed global operator relies on the spectral analysis of edge structure and exploits spatial as well as frequency-domain constraints derived from known geometrical models of monitored objects. A decision theoretic method for learning acceptance detection regions is presented. Applications of this attentional mechanism are demonstrated for several aerial image interpretation tasks for attentional as well as recognition purposes. Specific examples are described for detecting vehicle formations (such as convoys), qualifying the geometry of detected formations, or monitoring the occupancy of regions of interest (such as parking areas, roads, or open areas). Experiments and sensitivity analysis results are reported.     

Peptide-grafted nanodiamonds: preparation, cytotoxicity and uptake in cells

Nanodiamonds that were prepared by high pressure/high temperature were functionalized with biomolecules for biological applications. Nanodiamonds (NDs, < or =35 nm) that were coated by silanization or with polyelectrolyte layers were grafted with a fluorescent thiolated peptide via a maleimido function; this led to an aqueous colloidal suspension that was stable for months. These substituted NDs were not cytotoxic for CHO cells. Their capacity to enter mammalian cells, and their localisation inside were ascertained after labelling the nucleus and actin, by examining the cells by confocal, reflected light and fluorescence microscopy.     

Visual communication via trellis coding and transmission energyallocation

An unequal error protection approach for the reliable communication of visual information over additive white Gaussian noise channels is proposed and studied. This method relies on a bandwidth-efficient coded modulation scheme that employs selective channel coding and transmission energy allocation in conjunction with sequence maximum a posteriori soft-decision detection. Experimental results indicate that this scheme exhibits graceful performance degradation as the channel conditions deteriorate and provides substantial objective and subjective improvements over uncoded and equal-error protection systems. Coding gains of up to 4 dB in E_b /N_o are achieved     

Analyzing looming motion components from their spatiotemporalspectral signature

This paper addresses the use of spatio-temporal transform methods applied to the analysis of dynamic image sequences and the characterization of image motion. The image motion including a divergent component (resulting from a looming camera component) is analyzed in the spatio-temporal Mellin transform (MT) domain, resulting in the separation of the spectrum into two parts: a structural term corresponding to the spatial MT of the static image, and a kinematic term depending on time-to-collision (a motion support). We examine potential applications of this property for the recovery of image motion from integral image brightness measurements and the computation of time-to-collision using spatio-temporal MT analysis     

Tracking a new cell-penetrating (W/R) nonapeptide, through an enzyme-stable mass spectrometry reporter tag

We have designed a mass stable reporter (msr) tag with m/z over 500, trifluoroacetyl(alpha,alpha-diethyl)Gly-Lys(Nepsilonbiotin)-(D)Lys-Cys, for the quantification of the uptake and study of the degradation processes of cell-penetrating peptides (CPP), by matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. This tag was found stable in cell lysis conditions. Using a quantitative MALDI-TOF mass spectrometry analysis based method, an accurate tracking of a new CPP and of its degradation products could be done. (1) The new msr(W/R) nonapeptide (H-RRWWRRWRR-NH2) enters chinese hamster ovary (CHO) K1 cells with a kinetic reaching a steady state after 30-60 min of incubation. This plateau was stable for 4 h and decreased slowly afterward. (2) The peptide msr(W/R) nonapeptide was not cytotoxic over 48 h incubation with CHO cells. (3) After 1 h incubation, the msr(W/R) nonapeptide accumulated with a 3-fold higher concentration than the extracellularly added concentration (7.5 microM). (4) The intracellular quantification was accurate with less than 3% of the quantified peptide being potentially membrane-bound. (5) There was no leakage of the full-length CPP outside the cells. And, finally, (6) analysis of the degradation process of this new CPP suggests that the peptide did not traffick to lysosomes.     

Adaptive target detection in foliage-penetrating SAR images usingalpha-stable models

Detecting targets occluded by foliage in foliage-penetrating (FOPEN) ultra-wideband synthetic aperture radar (UWB SAR) images is an important and challenging problem. Given the different nature of target returns in foliage and nonfoliage regions and very low signal-to-clutter ratio in UWB imagery, conventional detection algorithms fail to yield robust target detection results. A new target detection algorithm is proposed that (1) incorporates symmetric alpha-stable (SalphaS) distributions for accurate clutter modeling, (2) constructs a two-dimensional (2-D) site model for deriving local context, and (3) exploits the site model for region-adaptive target detection. Theoretical and empirical evidence is given to support the use of the SalphaS model for image segmentation and constant false alarm rate (CFAR) detection. Results of our algorithm on real FOPEN images collected by the Army Research Laboratory are provided.     

An error resilient scheme for image transmission over noisychannels with memory

This article addresses the use of a joint source-channel coding strategy for enhancing the error resilience of images transmitted over a binary channel with additive Markov noise. In this scheme, inherent or residual (after source coding) image redundancy is exploited at the receiver via a maximum a posteriori (MAP) channel detector. This detector, which is optimal in terms of minimizing the probability of error, also exploits the larger capacity of the channel with memory as opposed to the interleaved (memoryless) channel. We first consider MAP channel decoding of uncompressed two-tone and bit-plane encoded grey-level images. Next, we propose a scheme relying on unequal error protection and MAP detection for transmitting grey-level images compressed using the discrete cosine transform (DCT), zonal coding, and quantization. Experimental results demonstrate that for various overall (source and channel) operational rates, significant performance improvements can be achieved over interleaved systems that do not incorporate image redundancy.     

Sequence-dependent enrichment of a model phosphopeptide: a combined MALDI-TOF and NMR study

The goal of this study was to detect and quantify by MALDI-TOF MS the phosphorylation of a peptide containing the recognition motif of the Protein Kinase C (PKC). Such model peptide can be used as a phosphorylation probe to follow intracellular kinase/phosphatase activities. This study allowed us to establish relationships between sequence specificities and affinity for TiO(2) or IMAC media. The peptide has the sequence biotin-GGGGCFRTPSFLKK-NH(2) in which the serine residue can be phosphorylated. Enrichment of the corresponding phosphopeptide, by the dedicated IMAC and TiO(2) affinity chromatography methods, proved inefficient. By combining MALDI-TOF and NMR data, we first showed that the lack of affinity of the phosphopeptide for TiO(2) was partly related to the basic property of its peptide sequence. Furthermore, the peptide shows local structuration around the P(9)- S(10) segment, with formation of a salt bridge between the guanidinium group of the R(7) side chain and the phosphate moiety. In conjunction with an inadequate position of the {biotin-G(4)} N-terminal tag, this local structure could shield the phosphate group, preventing interaction with TiO(2). To improve TiO(2) affinity, the peptide sequence was modified accordingly. The new sequences retained the biological properties while their enrichment by IMAC or TiO(2) became possible.     

The Efficacies of Cell‐Penetrating Peptides in Accumulating in Large Unilamellar Vesicles Depend on their Ability To Form Inverted Micelles

In this study, the direct translocation of cell‐penetrating peptides (CPPs) into large unilamellar vesicles (LUVs) was shown to be rapid for all the most commonly used CPPs. This translocation led within a few minutes to intravesicular accumulation up to 0.5 mM , with no need for a transbilayer potential. The accumulation of CPPs inside LUVs was found to depend on CPP sequence, CPP extravesicular concentration and phospholipid (PL) composition, either in binary or ternary mixtures of PLs. More interestingly, the role of anionic phospholipid flip‐flopping in the translocation process was ascertained. CPPs enhanced the flipping of PLs, and the intravesicular CPP accumulation directly correlated with the amount of anionic PLs that had been transferred from the external to the internal leaflet of the LUV bilayer, thus demonstrating the transport of peptide/lipid complexes as inverted micelles.