Morphological changes induced by colchicine in the chick optic cup in early stages of development. A stereological study

In this study chick embryo optic cups at HH stage 13 of development were analyzed under normal conditions and after inoculation with colchicine for 1, 2, 4, and 8 h. Several changes were seen after these periods of treatment: 1) modifications of the structure, with thicker regions in the cup and a general decrease in the total volume according to the duration of exposure to the drug (about 4 times less than normal, 5,035 x 10(3) microns 3 vs 1,334 x 10(3) microns 3 after 8 h of treatment); 2) enlargement of the ventricular cavity and its closure, due to failure of approximation of retinal and pigmentary layers; 3) failure of lens development, with delay and impairment of pit formation and deformation of all structures; lens volume was less than normal (about 4 times less, 2,148 x 10(3) microns 3 vs 658 x 10(3) microns 3 after 8 h of treatment); 4) a general segregation of the cells making up the structure, principally in the more active proliferating zones. The local alterations found are described.     

Using magnetic coupling to eliminate right half-plane zeros in boost converters

A dynamic analysis of the boost converter with an output filter reveals that magnetic coupling between inductors allows transfer of the zeros to the left half-plane of the control-to-output transfer function. Similar results requiring smaller magnetic components are obtained by combining magnetic coupling with damping of the output filter. The analysis is based on the application of the Routh-Hurtwitz's criterion to the numerator of the transfer function in order to derive the design conditions for the converter parameters. A design example illustrates the procedure, and experimental results verify the theoretical predictions. The application of these techniques will allow the design of high efficiency voltage boost-based regulators with dynamic behavior similar to buck-derived structures.     

Synthesis of loss-free resistors based on sliding-mode control and its applications in power processing

A systematic procedure to synthesize loss-free resistors (LFRs) based on sliding-mode control (SMC) of DC–DC switching converters is presented. Candidates for LFR operation are determined from the topological constraints imposed in a power converter by inducing sliding-mode motions. Two examples illustrate the use of the synthesized LFRs in power processing. The first one shows the application of the LFR as a pre-regulator stage for power factor correction (PFC) in single-phase circuits, while the second one describes its use for DC impedance matching in photovoltaic (PV) systems.     

Large-signal modeling and simulation of switching DC-DC converters

A general nonlinear continuous formulation procedure for large-signal analysis of switching DC-DC converters is presented. The method can be applied in either of the two conduction modes, and it is easily programmed for computer-aided analysis with small simulation time. A boost regulator operating in constant-frequency current-programmed mode is used to illustrate the application of the method. A stability graph is subsequently developed to facilitate the design of DC-DC switching regulators for large-signal applications. The graph provides an estimation of the values of input voltage and load resistance leading to a stable regulator behavior     

Study of the influence of notch radii and temperature on the probability of failure: A methodology to perform a combined assessment

The fracture assessment of notched components based on cracked components approaches leads to over‐conservative failure predictions. In the research literature, several approaches are proposed to overcome this problem using an apparent fracture toughness, . Nevertheless, most of these approaches are based on deterministic assumptions despite the large and variable scatter exhibited by for different notch radii (ρ) or temperatures (T). This paper proposes a methodology for deriving a probabilistic field including the effect of temperature on the failure of notched components. First, the theory of critical distances is applied to transform each apparent fracture toughness into the equivalent fracture toughness for ρ = 0. Then, the temperature is supposed to act as a scale effect in the Weibull cumulative distribution function of the equivalent fracture toughness, and the corresponding scale effect function is derived. Finally, the applicability of the proposed methodology is illustrated by an example using two ferritic‐pearlitic steels: S275JR and S355J2.     

Prediction of subharmonic oscillation in switching regulators: from a slope to a ripple standpoint

New exact critical conditions for predicting subharmonic instability in switching regulators are approximated by simple design-oriented expressions valid under practical conditions. These simplified expressions contain the ripple and slope information of the feedback control signal. Depending on the converter topology, the controller used and values of parasitic parameters, either the slope or the ripple can be dominant in predicting instability. A discussion on the validity of this interpretation is illustrated through six different examples of switching regulators using the concept of the spectral radius and the relative degree of the system loop. Using this approach, the boundary between the desired stable region and the subharmonic instability can be easily obtained. The theoretical results are validated by means of numerical simulations.     

The Stimulated Raman Spectrum of Symmetric 13C Cyanogen, 13C2N2

BACKGROUND: The authors examined the interaction of ketamine with recombinant mu, kappa, and delta opioid receptors and recombinant orphan opioid receptors expressed in Chinese hamster ovary cells (CHO-mu, CHO-kappa, CHO-delta, and CHO(ORL1), respectively). METHODS: CHO-mu, CHO-kappa, and CHO-delta membranes were incubated with the opioid receptor radioligand [3H]diprenorphine at room temperature. Ketamine (racemic, R(-) and S(+)) was included at concentrations covering the clinical range. CHO(ORL1) membranes were incubated with [125I]Tyr(14)nociceptin and racemic ketamine at room temperature. The effects of racemic ketamine and selective opioid receptor agonists (mu: [D-Ala2, MePhe4, Gly(ol)5] enkephalin (DAMGO); kappa: spiradoline or delta: [D-pen2, D-pen5] enkephalin (DPDPE)) on forskolin-stimulated cyclic adenosine monophosphate formation also were examined. Data are mean +/- SEM. RESULTS: Racemic ketamine increased the radioligand equilibrium dissociation constant for [3H]diprenorphine from 85+/-5 to 273+/-11, 91+/-6 to 154+/-16, and 372+/-15 to 855+/-42 pM in CHO-mu, CHO-kappa, and CHO-delta, respectively. The concentration of radioligand bound at saturation was unaffected. In CHO-mu and CHO-kappa cells, racemic ketamine did not slow the rate of naloxone-induced [3H]diprenorphine dissociation. Ketamine and its isomers also displaced [3H]diprenorphine binding to mu, kappa, and delta receptors in a dose-dependent manner, with pKi values for racemic ketamine of 4.38+/-0.02, 4.55+/-0.04, and 3.57+/-0.02, respectively. S(+)-ketamine was two to three times more potent than R(-)-ketamine at mu and kappa receptors. Racemic ketamine displaced [125I]Tyr(14)nociceptin with an estimated affinity constant of 0.5 mM. Racemic ketamine inhibited the formation of cyclic adenosine monophosphate (naloxone insensitive) in a dose-dependent manner (concentration producing 50% inhibition approximately 2 mM) in all cell lines, including untransfected CHO cells. Ketamine (100 microM) reversed DAMGO (mu) and spiradoline (kappa) inhibition of formation of cyclic adenosine monophosphate. CONCLUSIONS: Ketamine interacts stereoselectively with recombinant mu and kappa opioid receptors.     

Could Radiotherapy Effectiveness Be Enhanced by Electromagnetic Field Treatment?

One of the main goals in radiobiology research is to enhance radiotherapy effectiveness without provoking any increase in toxicity. In this context, it has been proposed that electromagnetic fields (EMFs), known to be modulators of proliferation rate, enhancers of apoptosis and inductors of genotoxicity, might control tumor recruitment and, thus, provide therapeutic benefits. Scientific evidence shows that the effects of ionizing radiation on cellular compartments and functions are strengthened by EMF. Although little is known about the potential role of EMFs in radiotherapy (RT), the radiosensitizing effect of EMFs described in the literature could support their use to improve radiation effectiveness. Thus, we hypothesized that EMF exposure might enhance the ionizing radiation effect on tumor cells, improving the effects of RT. The aim of this paper is to review reports of the effects of EMFs in biological systems and their potential therapeutic benefits in radiotherapy.     

Accurate analytical expressions for stripping voltammetry in the Henry adsorption limit

A strategy is developed to derive accurate analytical expressions for low-coverage cathodic stripping voltammetry. The procedure relies on the observation that diffusion affects the location of simulated voltammetric waves but not their shape, provided that physisorption of the analyte is negligible. As a proof of the generality of the proposed approach and having in mind the stripping of thiols, analytical solutions are derived for the cathodic stripping of monomers, dimers, and a mixture of monomers and dimers, whose reliability is proved by their comparison with numerically simulated voltammograms. Application to the deposition and reductive desorption of mercaptoacetic acid at a mercury electrode demonstrates that these approximate solutions can be used to get insights into the interfacial organization of incipient films. For this particular system, a transition from monomeric to dimeric behavior is identified upon increasing the thiol surface concentration. Further generalization of the proposed methodology is achieved by deriving an approximate analytical solution for thin-layer anodic stripping voltammetry, which is satisfactorily compared to the existing summation series solution.     

Destruction of Mycobacterium paratuberculosis, Salmonella spp., and Mycoplasma spp. in raw milk by a commercial on-farm high-temperature, short-time pasteurizer

The 2002 NAHM's Dairy Survey indicated that 87.2% of dairy farms in the United States feed waste milk to their neonatal calves. Although cost-effective, this practice can lead to increased calf morbidity and mortality due to ingestion of pathogenic agents. In an effort to reduce the risk of infection, dairy producers are implementing on-farm pasteurization of the waste milk as a control procedure before feeding the milk to calves. In the present study, the efficacy of a commercial high-temperature, short-time (HTST) on-farm pasteurizer unit to destroy Mycobacterium paratuberculosis, Salmonella enterica spp., and Mycoplasma spp. in raw milk was evaluated. Replicate experiments were run for 3 isolates of M. paratuberculosis, 3 serovars of Salmonella (derby, dublin, typhimurium); and 4 species of Mycoplasma (bovis, californicum, canadense, serogroup 7) at 2 different levels of experimental inoculation. In addition, HTST pasteurization experiments were performed on colostrum experimentally inoculated with M. paratuberculosis. After culture of the pasteurized milk samples, no viable M. paratuberculosis, Salmonella, or Mycoplasma were recovered, regardless of species, strain, or isolate. Pasteurization of colostrum was also effective in the destruction of M. paratuberculosis but resulted in an average 25% reduction in colostral immunoglobulin. These results suggest that HTST pasteurization is effective in generating a safer product to feed to young calves.