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Carballo V.M.B. Chefdeville M. Fransen M. van der Graaf H. Melai J. Salm C. Schmitz J. Timmermans J. 《Electron Device Letters, IEEE》2008,29(6):585-587
An unpackaged microchip is used as the sensing element in a miniaturized gaseous proportional chamber. This letter reports on the fabrication and performance of a complete radiation imaging detector based on this principle. Our fabrication schemes are based on wafer-scale and chip-scale postprocessing. Compared to hybrid-assembled gaseous detectors, our microsystem shows superior alignment precision and energy resolution, and offers the capability to unambiguously reconstruct 3D radiation tracks on the spot. 相似文献
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A flow-cytometric method with fluorescence-labeled monoclonal antibodies (MABs) against the low density lipoprotein (LDL) receptor (C7A MAB) or 3,3'-dioctadecylindocarbocyanin-iodide (DiI) LDL has been developed that allows the quantification of LDL receptors on leukocytes and the identification of patients with familial hypercholesterolemia (FH) within 48 hours. Leukocytes were isolated from 10 mL anticoagulated blood by density gradient centrifugation. To induce maximal expression of LDL receptors, mononuclear cells were preincubated with either phytohemagglutinine (PHA) or lipoprotein-deficient serum (LPDS). LPDS-treated monocytes provided a more homogeneous cell population with regard to LDL receptor activity than did the PHA-treated lymphocytes; they also provided a greater discrimination between the fluorescence of the receptor probes and cellular autofluorescence. The C7A MAB was able to compete for DiI LDL binding by about 40%. In competition with unlabeled LDL, DiI LDL revealed linear binding, indicating an affinity similar to native LDL. The binding characteristics of DiI LDL were also similar to 125I-LDL binding. LDL isolated from familial defective apolipoprotein B-100 was not able to compete for DiI LDL binding on monocytes, whereas native LDL reduced it by about 80%. In monocytes from FH heterozygous patients, the cellular mean fluorescence using either C7A MAB or DiI LDL at 4 degrees C was 30% to 70%; in FH homozygotes, cellular mean fluorescence was less than 20% of that in monocytes from normal individuals. In patients with familial defective apolipoprotein B-100 antibody binding was normal, but one patient's own LDL failed to compete with normal DiI LDL for 4 degrees C binding on U937 test monocytes. Patient monocytes having internalization defects showed normal 4 degrees C DiI LDL binding, but at 20 degrees C cell-associated fluorescence was reduced by about 40%. In our study 384 hypercholesterolemic patients (preselected according to serum cholesterol levels, clinical symptoms, and family history) were analyzed for LDL receptor expression using the C7A MAB-based assay. In 71.8% of the patients with cholesterol levels higher than 300 mg/dL, an LDL receptor deficiency was observed. Apolipoprotein E isoforms and lipoprotein[a] were found to be independent from the LDL receptor status. In some patients with high cholesterol levels but normal LDL receptor expression with the C7A MAB assay, LDL receptor defects could be diagnosed when either reduced binding or internalization of DiI LDL or familial defective apolipoprotein B-100 was detected.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
5.
An algorithm is presented for computing degrees of sequence conservation found among aligned amino acid sequences. Sequence identities are calculated for each position of an alignment and average identity values of neighboring positions are figured. The average identity value of the whole alignment is chosen as a limit to discriminate between well and less conserved sequence sections. A second algorithm is given to calculate the degree of divergence of individual sequences compared to the other sequences of the alignment. The approach is easy to use on microcomputers and gives an exact picture of sequence identities and differences in order to determine, first, protein regions of high functional or structural importance among homologous proteins, and, second, significant differences of single sequences that may contribute to individual properties of the analysed protein. The method is illustrated by an example analysing a sequence alignment of higher plant nitrate reductases. 相似文献
6.
Ingo Alig Dirk Lellinger Harald Oehler Sergey A. Adamovsky Christoph Schick 《Progress in Organic Coatings》2008,61(2-4):166-175
A new microcalorimeter with eight parallel channels using robust, low cost sensors for characterization of coatings and adhesives is described and first experiments on coatings and adhesives are presented. The calorimetric sensors are based on thin glass plates (20 mm × 20 mm, thickness 150 μm) with heater and thermocouple sputtered on the surfaces (calorimetric active area of about 9 mm2). The setup allows heating and cooling experiments as well as isothermal measurements in the temperature-modulated mode with up to eight sensors in parallel. The measured quantities are the real (C′p) and imaginary part (C″p) of the complex heat capacity (), the related absolute value of the heat capacity () and the heat flow . An industrial computer (NI PXI system) with specific software for calibration and data recording controls the electronic components. Sensors can be embedded in a temperature controlled oven (heating and cooling by Peltier elements) or alternatively in a climatic cabinet with controlled temperature and humidity.
The method has been applied successfully to monitoring of film formation of aqueous polymer dispersions (styrene-acrylate copolymer) and curing of coatings. 相似文献
7.
S Nielsen A Schmitz RE Knudsen J Dollerup CE Mogensen 《Canadian Metallurgical Quarterly》1994,87(12):747-754
In a double-blind, double-dummy, randomized, multi-centre study, the effects of bendroflumethiazide vs. enalapril on blood pressure, glycaemic control, lipoprotein concentrations and albuminuria were compared in non-proteinuric, hypertensive type 2 diabetic patients; they were treated for 20 weeks with either bendroflumethiazide 2.5-5.0 mg (n = 59) or enalapril 10-20 mg (n = 55). Age, fasting plasma glucose, HbA1c and BMI were similar in the groups. Systolic and diastolic blood pressure were reduced in both groups. Bendroflumethiazide was accompanied by minor but significant elevations in fasting plasma glucose and serum C-peptide. HbA1c was increased during both treatments. Lipoproteins and urinary albumin/creatinine ratio were stable. Bendroflumethiazide caused a decrease in serum potassium and an increase in serum urate. No significant correlations were observed between the decline in blood pressure and changes in the metabolic risk factors. Baseline levels of age, sex, BMI, blood pressure or urinary albumin/creatinine ratio were not related to changes in blood pressure, metabolic parameters or urinary albumin/creatinine ratio. 相似文献
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Experimental results for the electrodeposition of Ni-Zn alloys from chloride solutions, with addition of H3BO3 and without other additives, in a laboratory cell with a perforated nickel sheet cathode and with recirculating electrolyte are presented. The dependence of the zinc content in the alloy on the following operating conditions was investigated: cathodic current density, 1.0–20.0 A dm–2; temperature, 35–65°C; pH 1.5–5.5; total molarity,M
tot=M
Ni
2+
+M
Zn
2+
=1.1–2.8 M; and, molar ratio,P=M
Ni
2+
/M
Zn
2+
=1.0–15. Depending on the operating conditions the Zn content in the alloy varied over the range 22–88 mol%. In separate experiments galvanostatic polarization curves were measured in the direction of increasing and then decreasing cathodic current density in the range 0.1–20.0 A dm–2 with all other operating conditions as used for electroplating experiments. In all cases significant hysteresis effects were observed. It was found that the current efficiency for the electrodeposition of Ni-Zn alloys from chloride solutions as a function of the zinc content in the alloy showed a sharp minimum of about 55% atX
Zn=55 mol % irrespective of other operating conditions. 相似文献
10.
This letter presents an improved cue integration approach to reliably separate coherent moving objects from their background scene in video sequences. The proposed method uses a probabilistic framework to unify bottom-up and top-down cues in a parallel, "democratic" fashion. The algorithm makes use of a modified Bayes rule where each pixel's posterior probabilities of figure or ground layer assignment are derived from likelihood models of three bottom-up cues and a prior model provided by a top-down cue. Each cue is treated as independent evidence for figure-ground separation. They compete with and complement each other dynamically by adjusting relative weights from frame to frame according to cue quality measured against the overall integration. At the same time, the likelihood or prior models of individual cues adapt toward the integrated result. These mechanisms enable the system to organize under the influence of visual scene structure without manual intervention. A novel contribution here is the incorporation of a top-down cue. It improves the system's robustness and accuracy and helps handle difficult and ambiguous situations, such as abrupt lighting changes or occlusion among multiple objects. Results on various video sequences are demonstrated and discussed. (Video demos are available at http://organic.usc.edu:8376/ approximately tangx/neco/index.html .). 相似文献