排序方式: 共有5条查询结果,搜索用时 15 毫秒
1
1.
Farah Perveen Mughal Ann Christina Bergmann Ha Uyen Buu Huynh Sarah Hyllekvist Jrgensen Inaam Mansha Meliha Kesmez Patrick Mark Schürch Alexandre Pierre Andr Theocharides Paul Robert Hansen Tina Friis Morten Orebo Holmstrm Evaldas Ciplys Rimantas Slibinskas Peter Hjrup Gunnar Houen Nicole Hartwig Trier 《International journal of molecular sciences》2022,23(12)
Myeloproliferative Neoplasms (MPNs) constitute a group of rare blood cancers that are characterized by mutations in bone marrow stem cells leading to the overproduction of erythrocytes, leukocytes, and thrombocytes. Mutations in calreticulin (CRT) genes may initiate MPNs, causing a novel variable polybasic stretch terminating in a common C-terminal sequence in the frameshifted CRT (CRTfs) proteins. Peptide antibodies to the mutated C-terminal are important reagents for research in the molecular mechanisms of MPNs and for the development of new diagnostic assays and therapies. In this study, eight peptide antibodies targeting the C-terminal of CRTfs were produced and characterised by modified enzyme-linked immunosorbent assays using resin-bound peptides. The antibodies reacted to two epitopes: CREACLQGWTE for SSI-HYB 385-01, 385-02, 385-03, 385-04, 385-07, 385-08, and 385-09 and CLQGWT for SSI-HYB 385-06. For the majority of antibodies, the residues Cys1, Trp9, and Glu11 were essential for reactivity. SSI-HYB 385-06, with the highest affinity, recognised recombinant CRTfs produced in yeast and the MARIMO cell line expressing CRTfs when examined in Western immunoblotting. Moreover, SSI-HYB 385-06 occasionally reacted to CRTfs from MPN patients when analysed by flow cytometry. The characterized antibodies may be used to understand the role of CRTfs in the pathogenesis of MPNs and to design and develop new diagnostic assays and therapeutic targets. 相似文献
2.
Ciplys D. Rimeika R. Shur M.S. Gaska R. Sereika A. Yang J. Asif Khan M. 《Electronics letters》2002,38(3):134-135
A new solar-blind UV detector based on a GaN surface acoustic wave delay line oscillator is reported. The illumination by an artificial UV source in the presence of sunlight increased the RF spectral line width of the oscillator by almost an order of magnitude, which is attributed to the different noise spectra of these light sources. The output of this detector is a radio signal, which makes it especially attractive for remote sensing applications 相似文献
3.
Ciplys D. Rimeika R. Suarez I. Lifante G. Shur M.S. Aulas A. 《Electronics letters》2006,42(22):1294-1295
The acousto-optic control of light in Zn-diffused LiNbO3 optical waveguides is demonstrated. The guided optical mode diffraction by the surface acoustic waves is consistent with the Klein and Cook model for the intermediate diffraction regime. The SAW induced refractive index change is 1.5times10-4 at acoustic power 130 mW. The Zn-diffused lithium niobate waveguides appear to be good candidates for integrated light-control devices based on acousto-optic interaction 相似文献
4.
Nicole Hartwig Trier Niccolo Valdarnini Ilaria Fanelli Paolo Rovero Paul Robert Hansen Claus Schafer-Nielsen Evaldas Ciplys Rimantas Slibinskas Flemming Pociot Tina Friis Gunnar Houen 《International journal of molecular sciences》2022,23(8)
Two isoforms of the glutamate decarboxylase (GAD) enzyme exist, GAD65 and GAD67, which are associated with type 1 diabetes (T1D) and stiff-person syndrome (SPS), respectively. Interestingly, it has been reported that T1D patients seldom develop SPS, whereas patients with SPS occasionally develop T1D. In addition, coxsackievirus B4 (CVB4) has previously been proposed to be involved in the onset of T1D through molecular mimicry. On this basis, we aimed to examine antibody cross-reactivity between a specific region of GAD65 and GAD67, which has high sequence homology to the nonstructural P2C protein of CVB4 to determine potential correlations at antibody level. Monoclonal peptide antibodies generated in mice specific for a region with high similarity in all three proteins were screened for reactivity along with human sera in immunoassays. In total, six antibodies were generated. Two of the antibodies reacted to both GAD isoforms. However, none of the antibodies were cross-reactive to CVB, suggesting that antibody cross-reactivity between GAD65 and CVB, and GAD67 and CVB may not contribute to the onset of T1D and SPS, respectively. 相似文献
5.
Bu G Ciplys D Shur M Schowalter LJ Schujman S Gaska R 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2006,53(1):251-254
The surface acoustic wave velocity has been measured on a-plane (c-propagation) and c-plane oriented bulk aluminum nitride (AlN) single crystals using the S11-parameter method in the frequency range 160-360 MHz. The SAW velocity is 5760 m/s for both orientations. From comparison of this value with the simulations using various elastic constants of AlN available in literature, we estimated the elastic constant C44 to be 122 +/- 1 GPa. 相似文献
1