Effect of refrigerant charge on the performance of air conditioning systems

An air conditioning system operates in an optimal condition if the system is fully charged with a specified amount of refrigerant. Poor field maintenance or refrigerant leakage causes low level of charge resulting in a lower thermal performance and higher operating cost. An experimental investigation was conducted to study the effect of low charge level of R‐22 on the performance of a 3‐ton residential air conditioning system. The experimental results show that if a system is undercharged to 90 per cent then the effect is small: a 3.5 per cent reduction in cooling capacity and a 2 per cent increase in the coefficient of performance (COP). However, the system performance suffers serious degradation if the level of charge drops below 80 per cent. An ice layer formed on the outer surface of the cooling coil impedes the heat transfer between the warm air and cold refrigerant vapour. An economic analysis shows that the cost of properly charging an under‐charged system which is at an 85 per cent charge level, can pay for itself in savings in a short period of 3–4 months. Copyright © 2001 John Wiley & Sons, Ltd.     

A state observer for the Virgo inverted pendulum

We report an application of Kalman filtering to the inverted pendulum (IP) of the Virgo gravitational wave interferometer. Using subspace method system identification techniques, we calculated a linear mechanical model of Virgo IP from experimental transfer functions. We then developed a Kalman filter, based on the obtained state space representation, that estimates from open loop time domain data, the state variables of the system. This allows the observation (and eventually control) of every resonance mode of the IP mechanical structure independently.     

A feasibility study of steam-jet refrigeration

In this paper a feasibility study of steam-jet refrigeration is conducted. First and second law analyses as well as the economics of a steam-jet refrigeration system are presented. Thermodynamic parameters investigated include the coefficient of performance (COP), refrigerating effect, condenser heat rejection, motive steam requirements, second law efficiency, and system irreversibility. Economic parameters investigated include equipment costs, space needs, power requirements, and installed and operational costs. © 1998 John Wiley & Sons, Ltd.     

Physicochemical, microbiological and coagulating properties of ewe's milk produced on the Calabrian Mount Poro plateau

Three hundred individual ewe's milks and six bulk milks were analysed over two seasons. The results show that in spring but not in winter the milks examined conform substantially to the standards laid down by Italian legislation. Nevertheless, the data acquired constitute a good starting point from which the quality of ewe's milk produced on the Calabrian Mount Poro plateau should be improved, by performing selection of sheep for mastitis resistance based on somatic cell count, improving the hygiene standards at the dairy, using selected autochthonous lactic starter cultures and, only if strictly necessary, thermal treatment of the milk.     

Palladium N‐Heterocyclic Carbene Catalysts for the Ultrasound‐Promoted Suzuki–Miyaura Reaction in Glycerol

Novel palladium N‐heterocyclic‐carbene (NHC)‐based complexes with 3,4,5‐trimethoxybenzyl, alkyl and sulfonate N‐substituents were obtained and fully characterized. The new complexes were used as pre‐catalysts in the Suzuki–Miyaura coupling of various aryl halides/boron sources in glycerol under pulsed‐ultrasound (P‐US) activation. High yields were obtained under mild reaction conditions, without formation of undesired by‐products. The pure final cross‐coupling products were easily recovered without column chromatography and the catalytic/solvent system could be recycled. TEM (transmission electron microscopy) and XPS (X‐ray photoelectron spectroscopy) were used to characterize the nanoparticles and to investigate the fate of the catalysts.     

A Framework for the Evaluation of Trainee Performance in Cyber Range Exercises

This paper proposes a novel approach for the evaluation of the performance achieved by trainees involved in cyber security exercises implemented in modern cyber ranges. Our main contributions include: the definition of a distributed monitoring architecture for gathering relevant information about trainees activities; an algorithm for modeling the trainee activities using directed graphs; novel scoring algorithms, based on graph operations, that evaluate different aspects (speed, precision) of a trainee during an exercise. With respect to previous work, our proposal allows to measure exactly how fast a user is progressing towards an objective and where he does wrong. We highlight that this is currently not possible in the most popular cyber ranges.

     

Antiviral activity and toxicity of fialuridine in the woodchuck model of hepatitis B virus infection

The crystal structure of the mouse major histocompatibility complex (MHC) class I molecule H-2Dd with an immunodominant peptide, designated P18-I10 (RGPGRAFVTI), from human immunodeficiency virus envelope glycoprotein 120 was determined at 3.2 A resolution. A novel orientation of the alpha3 domain of Dd relative to the alpha1/alpha2 domains results in significantly fewer contacts between alpha3 and beta2-microglobulin compared with other MHC class I proteins. Four out of ten peptide residues (P2 Gly, P3 Pro, P5 Arg and P10 Ile) are nearly completely buried in the Dd binding groove. This is consistent with previous findings that Dd exploits a four-residue binding motif comprising a glycine at P2, a proline at P3, a positively charged residue at P5, and a C-terminal hydrophobic residue at P9 or P10. The side-chain of P5 Arg is directed toward the floor of the predominantly hydrophobic binding groove where it forms two salt bridges and one hydrogen bond with Dd residue Asp77. The selection of glycine at P2 appears to be due to a narrowing of the B pocket, relative to that of other class I molecules, caused by Arg66 whose side-chain folds down into the binding cleft. Residue P3 Pro of P18-I10 occupies part of pocket D, which in Dd is partially split by a prominent hydrophobic ridge in the floor of the binding groove formed by Trp97 and Trp114. Residues P6 through P9 form a solvent-exposed bulge, with P7 Phe protruding the most from the binding groove and thereby probably constituting a major site of interaction with T cell receptors. A comparison of H-2Dd/P18-I10 with other MHC class I/peptide complexes of known structure provides insights into the possible basis for the specificity of the natural killer cell receptor Ly-49A for several related class I molecules.     

Inhibition of influenza virus hemagglutinin-mediated membrane fusion by a compound related to podocarpic acid

Entry of influenza virus into the host cell is dependent on the fusion of the viral envelope with the endosomal membrane and is mediated by a low-pH-induced change of the viral hemagglutinin (HA) to a conformation that is fusogenic. A compound related to podocarpic acid (180299) was identified that inhibits multicycle replication of influenza A/Kawasaki/86 (H1N1) virus in culture. Treatment of Madin-Darby canine kidney (MDCK) cells with 180299 at 1 h before infection resulted in the inhibition of viral protein synthesis. Addition of 20 microgram of 180299/ml at 1 h p.i. had no effect, indicating that 180299 affects an early step of the influenza viral replication cycle. Genetic analysis of reassortants between sensitive and resistant viruses demonstrated that hemagglutinin (HA) conferred the 180299-resistant (180299(r)) phenotype. Twelve independent isolates of influenza A/Kawasaki/86 were selected for resistance to 180299, and sequence analysis revealed that each of these viruses contained amino acid substitutions in the HA. These mutations are dispersed throughout the HA primary amino acid sequence and cluster in one of two regions: the interface between HA1 and HA2 and in a region near the fusion domain of HA2. When compared with the parent virus, the pH-of-inactivation of the resistant mutants was increased by 0.3 to 0.6 pH unit, suggesting that the mutant HAs undergo the conformational change at an elevated pH. Fusion of human erythrocytes to MDCK cells infected with parent influenza A/Kawasaki/86 was inhibited by 180299 (0.1-10 microgram/ml) in a concentration-dependent manner, whereas fusion of erythrocytes to MDCK cells infected with 180299(r) mutants was not affected. These results suggest that 180299 interacts with the neutral pH conformation of influenza A HA and prevents the low-pH-induced change of HA to its fusogenic conformation.     

A Ruthenium Complex‐Catalyzed Cyclotrimerization of Halodiynes with Nitriles. Synthesis of 2‐ and 3‐Halopyridines

Monohalo‐ and dihalodiynes efficiently undergo [2+2+2] cyclotrimerization with nitriles in the presence of a catalytic amount of the ruthenium complex Cp*RuCl(cod) (10 mol%) to afford the corresponding halopyridines under ambient conditions in good isolated yields (up to 90%). The halopyridines are formed as two separable regioisomers. This is the first example of a direct synthesis of halopyridines from haloalkynes and nitriles.