The formation of thermochemically stable Schottky barriers on p-Cd0.95Zn0.05Te using Titanium

The electrical properties of Ti-based contacts on p-Cd_0.95Zn_0.05Te (CZT) were investigated. Ti contacts with a Cd or a Te interlayer were produced and compared with the pure Ti contact using current-voltage characteristics (I–V). The barrier heights can be perceived using data on the interface reactions and the phase formation in direct contact with CZT. The percept of the dominating influence of phase formation could be validated for stoichiometric CZT substrate surfaces and also for etched (Te-rich) substrates. It was found that Ti, i.e. the Ti₃Te₄ phase that forms at the interface, produces high barriers in the range of 1 eV. In addition, various Au capped Ti-contacts were investigated. Thermally stable, near-ideal Au/Ti-contacts with a barrier height of 0.76 eV were produced.     

Partial Reduction in BRCA1 Gene Dose Modulates DNA Replication Stress Level and Thereby Contributes to Sensitivity or Resistance

BRCA1 is a well-known breast cancer risk gene, involved in DNA damage repair via homologous recombination (HR) and replication fork protection. Therapy resistance was linked to loss and amplification of the BRCA1 gene causing inferior survival of breast cancer patients. Most studies have focused on the analysis of complete loss or mutations in functional domains of BRCA1. How mutations in non-functional domains contribute to resistance mechanisms remains elusive and was the focus of this study. Therefore, clones of the breast cancer cell line MCF7 with indels in BRCA1 exon 9 and 14 were generated using CRISPR/Cas9. Clones with successful introduced BRCA1 mutations were evaluated regarding their capacity to perform HR, how they handle DNA replication stress (RS), and the consequences on the sensitivity to MMC, PARP1 inhibition, and ionizing radiation. Unexpectedly, BRCA1 mutations resulted in both increased sensitivity and resistance to exogenous DNA damage, despite a reduction of HR capacity in all clones. Resistance was associated with improved DNA double-strand break repair and reduction in replication stress (RS). Lower RS was accompanied by increased activation and interaction of proteins essential for the S phase-specific DNA damage response consisting of HR proteins, FANCD2, and CHK1.     

Crossing the divide: Infants discriminate small from large numerosities.

Although young infants have repeatedly demonstrated successful numerosity discrimination across large sets when the number of items in the sets changes twofold (E. M. Brannon, S. Abbott, & D. J. Lutz, 2004; J. N. Wood & E. S. Spelke, 2005; F. Xu & E. S. Spelke, 2000), they consistently fail to discriminate a twofold change in number when one set is large and the other is small (     

Accuracy of Galerkin finite elements for groundwater flow simulations in two and three‐dimensional triangulations

In standard finite element simulations of groundwater flow the correspondence between hydraulic head gradients and groundwater fluxes is represented by the stiffness matrix. In two‐dimensional problems the use of linear triangular elements on Delaunay triangulations guarantees a stiffness matrix of type M. This implies that the local numerical fluxes are physically consistent with Darcy's law. This condition is fundamental to avoid the occurrence of local maxima or minima, and is of crucial importance when the calculated flow field is used in contaminant transport simulations or pathline evaluation. In three spatial dimensions, the linear Galerkin approach on tetrahedra does not lead to M‐matrices even on Delaunay meshes. By interpretation of the Galerkin approach as a subdomain collocation scheme, we develop a new approach (OSC, orthogonal subdomain collocation) that is shown to produce M‐matrices in three‐dimensional Delaunay triangulations. In case of heterogeneous and anisotropic coefficients, extra mesh properties required for M‐stiffness matrices will also be discussed. Copyright © 2001 John Wiley & Sons, Ltd.     

Gold(I)‐Catalysed Cyclisation of Alkynoic Acids: Towards an Efficient and Eco‐Friendly Synthesis of γ‐, δ‐ and ϵ‐Lactones

The improved synthesis of γ‐, δ‐ and ϵ‐lactones using a dinuclear N‐heterocyclic carbene (NHC)‐gold(I) catalyst is reported. This solvent‐free process provides access to γ‐ and δ‐lactones in high regio‐ and stereoselectivity. Reactions were performed at low catalyst loadings and without the need for any additives. The use of a digold pre‐catalyst provides a new synthetic route to functionalised ϵ‐lactones, poorly accessible using previous methodologies.

     

Conserved and distinct roles of kreisler in regulation of the paralogous Hoxa3 and Hoxb3 genes

During anteroposterior patterning of the developing hindbrain, the anterior expression of 3' Hox genes maps to distinct rhombomeric boundaries and, in many cases, is upregulated in specific segments. Paralogous genes frequently have similar anterior boundaries of expression but it is not known if these are controlled by common mechanisms. The expression of the paralogous Hoxa3 and Hoxb3 genes extends from the posterior spinal cord up to the rhombomere (r) 4/5 boundary and both genes are upregulated specifically in r5. However, in this study, we have found that Hoxa3 expression is also upregulated in r6, showing that there are differences in segmental expression between paralogues. We have used transgenic analysis to investigate the mechanisms underlying the pattern of segmental expression of Hoxa3. We found that the intergenic region between Hoxa3 and Hoxa4 contains several enhancers, which summed together mediate a pattern of expression closely resembling that of the endogenous Hoxa3 gene. One enhancer specifically directs expression in r5 and r6, in a manner that reflects the upregulation of the endogenous gene in these segments. Deletion analysis localized this activity to a 600 bp fragment that was found to contain a single high-affinity binding site for the Maf bZIP protein Krml1, encoded by the kreisler gene. This site is necessary for enhancer activity and when multimerized it is sufficient to direct a kreisler-like pattern in transgenic embryos. Furthermore the r5/r6 enhancer activity is dependent upon endogenous kreisler and is activated by ectopic kreisler expression. This demonstrates that Hoxa3, along with its paralog Hoxb3, is a direct target of kreisler in the mouse hindbrain. Comparisons between the Krml1-binding sites in the Hoxa3 and Hoxb3 enhancers reveal that there are differences in both the number of binding sites and way that kreisler activity is integrated and restricted by these two control regions. Analysis of the individual sites revealed that they have different requirements for mediating r5/r6 and dorsal roof plate expression. Therefore, the restriction of Hoxb3 to r5 and Hoxa3 to r5 and r6, together with expression patterns of Hoxb3 in other vertebrate species suggests that these regulatory elements have a common origin but have later diverged during vertebrate evolution.     

Electron and hole mobility enhancement in strained SOI by waferbonding

N- and p-MOSFETs have been fabricated in strained Si-on-SiGe-on-insulator (SSOI) with high (15-25%) Ge content. Wafer bonding and H-induced layer transfer techniques enabled the fabrication of the high Ge content SiGe-on-insulator (SGOI) substrates. Mobility enhancement of 50% for electrons (with 15% Ge) and 15-20% for holes (with 20-25% Ge) has been demonstrated in SSOI MOSFETs. These mobility enhancements are commensurate with those reported for FETs fabricated on strained silicon on bulk SiGe substrates