Cytochemical characteristics of the golgi apparatus

Lectinocytochemistry provides a useful tool for localizing subcompartments of the complex reticular apparatus of Golgi. The technique is based on interactions of lectins with glycoconjugates present in the limiting membranes and luminal spaces of Golgi elements. Application of a series of lectins of different sugar specificities permits a differentiation between Golgi subcompartments containing glycoconjugates with different oligosaccharide side chains. These may be (a) different glycoconjugates or (b) glycoconjugates at different stages during synthesis or repair of their glycans. The lectinocytochemical studies with mannose-, glucose-, N-acetyl-glucosamine-, N-acetylgalactosamine-, galactose-, fucose-, and sialic acid-recognizing lectins revealed predominating patterns that labeled distinct, i.e., cis, medial, trans, and transmost, regions of the Golgi apparatus. A further refinement could be achieved by differential lectin-inhibition that enables a dissection of lectin binding reactions on the basis of their binding affinities. High-affinity binding reactions showed that subcompartments are not necessarily confined to one single Golgi subregion and may change their position from one to another subregion. Some of the patterns observed may be interpreted in relation to certain steps during synthesis and modifications of glycans.     

40 Gbit/s limiting output buffer in 80 nm CMOS

A 40 Gbit/s 1V limiting output buffer for an AC-coupled 50 /spl Omega/ load with a differential output swing of 660 mV and a gain of 18 dB is presented. A power consumption of only 24 mW and a simulated risetime of 11 ps are achieved by means of a systematic buffer optimisation.     

The yeast growth control gene GRC5 is highly homologous to the mammalian putative tumor suppressor gene QM

We isolated the Saccharomyces cerevisiae GRC5 (gr owth control) gene by functional complementation in vivo of a ts (t emperature s ensitive) mutation. Phenotypic analysis suggested involvement of GRC5 in cell growth and proliferation. Mutant cells arrest their cell cycles after one to three cell divisions predominantly as mother cells with a large bud. In the region of the septum, a massive accumulation of cell wall material is observed. The mother and daughter nuclei are well separated and spindles are no longer present, while the cytoskeleton is of aberrant appearance. Arrested cells do not perform protein synthesis and are unable to mate. Furthermore, grc5-1^ts cells rapidly lose viability at the restrictive temperature (37°C) only on full media, but not under nitrogen-starvation conditions, indicating that proper response to this nutrient limitation is still intact in mutant cells after cell cycle arrest. The sequence of GRC5 translates into a basic protein of 221 amino acids with a corresponding M_r of 25·4 kDa. GRC5 is a member of the highly conserved QM gene family, members of which have been reported from plants, invertebrates and vertebrates. The amino acid sequence of GRC5 over its entire length is more than 60% identical to the human QM protein, expression of which is associated with loss of the tumorigenic phenotype in a cell line derived from Wilms' tumor, a malignancy of the embyronic kidney. Here, we show that GRC5 is an essential yeast gene, the function of which as inferred from analysis of the grc5-1^ts mutant is crucial for establishment of proper cytoskeletal structure and regulation of growth in yeast cells.     

紫外分光光度法测定PAN纤维中的DMSO含量

用蒸馏水为萃取剂萃取出聚丙烯腈纤维中的DMSO,然后用紫外分光光度法测定其含量。测定的最佳线性浓度为:4．5× 10-10～1．6×10-9mol／L,DMSO最大吸收波长为208 nm, 检测限为1．9×10-9 mol/L,相关系数为0．999 8,相对标准偏差小于0．5％;与其它常用的测定方法相比,解决了从纤维中分离DMSO的难题,具有简便、快速、准确和低成本的特点。     

Bolus ingestion of white and green tea increases the concentration of several flavan‐3‐ols in plasma,but does not affect markers of oxidative stress in healthy non‐smokers

White tea (WT) is rich in flavan‐3‐ols as green tea (GT) and might provide health protective effects due to the strong antioxidant properties of flavan‐3‐ols. Since intervention studies with WT are lacking, we evaluated the effects of WT consumption on antioxidant status, antioxidant capacity and biomarkers of oxidative stress compared to water and GT. After an overnight fast, 70 healthy non‐smokers were randomized to consume 600 mL of WT, GT or water (control). Plasma (epi‐)catechin and epi(gallo)catechingallate, antioxidant capacity (Folin assay, trolox equivalent antioxidant capacity test), 8‐iso‐prostaglandin F_2α, ascorbic acid and uric acid were determined before and several times within 8 h after consumption. DNA strand breaks were measured in vivo and ex vivo (H₂O₂ stimulation) in leukocytes. Plasma flavan‐3‐ols significantly increased after WT and GT ingestion. Trolox equivalent antioxidant capacity was lower after 5 h in controls versus WT (p=0.031) and GT (p=0.005). Folin‐Ciocalteu reducing capacity, ascorbic and uric acid as well as markers of oxidative stress (8‐iso‐prostaglandin‐F_2α, DNA strand breaks) were not affected by the beverages. A short‐term increase of catechins does not change plasma antioxidant capacity in healthy subjects. Conclusions with respect to health protective effects of WT and GT on the basis of these biomarkers can, thus, not be drawn.     

60-GHz SOI CMOS traveling-wave amplifier with NF below 3.8 dB from 0.1 to 40 GHz

In this paper, the design and the results of a CMOS traveling-wave amplifier (TWA) optimized for minimum noise figure is presented. Design tradeoffs and optimization guidelines for maximum operation frequency, gain and minimum noise are discussed by means of analytical calculations and simulations. The MMIC is fabricated using digital 90-nm silicon on insulator (SOI) technology and requires a chip area of only 0.3 mm/sup 2/. At a supply voltage of 2 V and a supply current of 66 mA, a gain of 9.7 dB/spl plusmn/1.6 dB is measured over a frequency range from 10 to 59 GHz. Toward dc, the gain increases up to 16 dB. The unity gain cutoff frequency is 71 GHz. At 20 and 40 GHz, the circuit has a 1-dB output compression point of 12.5 and 9.5 dBm, respectively. From 0.1 to 40 GHz, a noise figure below 3.8 dB is measured. The results are achieved at source/load impedances of 50 /spl Omega/ and include the pad parasitics. To the author's knowledge, the TWA has by far the lowest noise figure achieved for a silicon-based amplifier with comparable bandwidth.     

Evaluation of a semi-cylindrical solenoid as an applicator forradio-frequency hyperthermia

A modified solenoid having a semi-circular cross section is evaluated as an applicator for radio-frequency heating of biological materials. The operating efficiency of this applicator and the distribution of the electric field established within a simulated biological sample are determined for a number of modes of excitation. It is shown that uniform heating to a substantial depth is possible at efficiencies in excess of 80 percent.     

A cutting force model for finishing processes using helical end mills with significant runout

Analytical cutting force models play an important role in a wide array of simulation approaches of milling processes. The accuracy of the simulated processes directly depends on the predictive power of the applied cutting force model, which may vary under specific circumstances. End milling processes with small radial cutting depths, e.g. finishing processes, are particularly problematic. In this case, the tool runout, which is usually neglected in established cutting force models, can become quite significant. Within this article, well-known cutting force models are implemented for runout-prone finishing processes and modified by integrating additional parameters. A method is presented for how these additional runout parameters can be efficiently determined alongside commonly used cutting coefficients. For this purpose, a large number of milling experiments have been performed where the cutting forces were directly measured using a stationary dynamometer. The measured cutting forces were compared with the simulated cutting forces to verify and assess the modified model. By using the presented model and calibration method, cutting forces can be accurately predicted even for small radial cutting depths and significant tool runout.