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1.
G Van Zant SA Rummel MR Koller DB Larson I Drubachevsky M Palsson SG Emerson 《Canadian Metallurgical Quarterly》1994,20(2-3):482-90; discussion 491
Umbilical cord blood (UCB) and mobilized peripheral blood (MPB) provide an alternate source to bone marrow for transplantation. Expansion in vitro of stem/progenitor cell populations from these sources may provide adult-sized grafts otherwise not attainable because of the limited cell numbers available in the case of UCB or because of numerous rounds of apheresis required for sufficient MPB cells. We asked whether continuous perfusion culture could be employed in ex vivo expansion to produce clinically relevant numbers of stem/progenitor cells from these sources. To evaluate MPB, 1-10 million leukocytes, from patients who had received either granulocyte colony-stimulating factor (G-CSF) or cyclophosphamide and granulocyte-macrophage colony-stimulating factor (GM-CSF), were inoculated into bioreactors, with or without irradiated, allogeneic stroma. The growth factor combination in the perfusion medium consisted of interleukin-3 (IL-3), stem cell factor (SCF), GM-CSF and erythropoietin (Epo). Under the best conditions tested, total cell numbers, granulocyte-macrophage colony-forming units (CFU-GM), and long-term culture-initiating cell (LTC-IC) populations were expanded by about 50-, 80-, and 20-fold, respectively, over 14 days. At low cell inocula (1 million), the presence of stroma enhanced the expansion of total cells and CFU-GM but not of LTC-IC. When SCF was not included in the medium, both total cells and CFU-GM expanded to a much lesser extent, but again the expansion of LTC-IC was not affected. At the higher cell inoculum (10 million), expansions of total cells and CFU-GM were equivalent with or without stroma. To evaluate UCB, cells were placed into bioreactors with or without irradiated, allogeneic stroma, and the bioreactors were perfused with medium containing the four standard growth factors. After 6-14 days, in several independent experiments, 20-24 million cells were harvested from bioreactors perfused with SCF-containing medium, irrespective of the presence or absence of preformed stroma. Similarly, in reactors perfused with SCF-containing medium (with or without stroma), an average 40- to 60-fold expansion of CFU-GM was obtained, yielding an average of 1.5-1.8 x 10(5) CFU-GM per reactor. Harvested cells were thus up to 40-fold enriched in CFU-GM in comparison to the inoculum. In the absence of SCF, cell expansions averaged 1.5- to 2-fold, and CFU-GM were expanded only 10- to 14-fold by day 14. As before, the presence of preformed stroma did not affect either cell or CFU-GM yields, provided the cell inoculum was at least 4.5 million cells.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
2.
Fbio Gomes Costa Elias Andrade Braga Soraia Teixeira Brando Alexandre de Freitas Espeleta Zênis Novais da Rocha Lílian Maria Tosta Simplício Emerson Andrade Sales 《Applied Catalysis A: General》2005,290(1-2):221-226
Infrared and UV–vis studies of metallocene immobilization on silica are reported here. The results have indicated changes in the Zr coordination sphere of metallocene depending on the immobilization route used. The reaction of [(Cp)2ZrCl2] with silica formed [(Cp)2ZrCl]+[SiO]− species. The same metallocene, reacting with TMA modified silica, formed monomethylated and dimethylated species by the substitution of chloro for methyl ligands, stabilized on the surface by interaction with “MAO-like” species (methylaluminoxane, MAO). These monomethylated and dimethylated cationic zirconium species are the active centers for the polymerization reaction. Different order of TMA addition in the silica modification step generated surface species of a similar nature, differing in their relative quantities. The highest amount of these active species was obtained when the support was added to the TMA solution rather than adding the TMA solution to the silica support. This was the most significant parameter affecting catalytic activity in ethylene polymerization. 相似文献
3.
Ultrastructure of sperm and eggs of the ocean pout (Macrozoarces americanus L.), an internally fertilizing marine teleost, was examined by scanning and transmission electron microscopy. The results showed that the sperm do not have an acrosome but have a very long mid-piece (one to two times the sperm head length) containing numerous well-developed elongated mitochondria. The sperm also have two tails (is biflagellate) each consisting of nine peripheral and one central pair (9 +/- 2) of microtubules. This long mid-piece and the biflagellate nature of the sperm appear to be associated with the long life-span of the sperm and with sperm dispersal in the ovary to fertilize the eggs internally. The ocean pout eggs are enveloped by a porous chorionic membrane similar to that found in other teleosts but have two micropyles, a condition likely related to a mechanism of egg fertilization which increases the egg fertility in the presence of low sperm numbers. Following insemination, some biochemically undefined excretions appeared on the surface of fertilized eggs and led to the acquisition of adherent capability of the eggs which formed a tightly associated egg mass in sea water. 相似文献
4.
Angiographic procedures are technically difficult when soft-tissue scarring and deformity are present at the site of vascular access. This case demonstrates the rare complication of catheter-sheath separation during an attempted coronary arteriogram. The patient had deformity and soft-tissue scarring due to bilateral lower-extremity amputation secondary to blast injuries sustained in Vietnam. 相似文献
5.
6.
Emerson Judith; Bertoch Michael R.; Checketts Keith T. 《Canadian Metallurgical Quarterly》1994,31(1):109
Explored the construct validity of transactional analysis (TA) ego state concepts. Ego states are defined as coherent systems of thoughts, feelings, and behaviors. 65 university students seeking therapy and 95 university students not receiving therapy completed the Adjective Checklist (ACL) and the Brief Symptom Inventory (BSI). Ego state scores from the ACL correlated with BSI psychological distress in predicted directions. Four of the 5 TA ego state scores differentiated Ss seeking therapy from Ss not seeking therapy. Furthermore, ego state scores changed over the course of therapy, indicating a reduction of distress. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
7.
1引言2006年12月11日是我国正式加入世界贸易组织五周年,也是开放基础电信业务二周年。外资运营商进入中国市场的步伐也有加快的迹象。澳大利亚电讯(亚洲)总裁、澳大利亚电讯(中国)CEO贝裕铭日前表示,澳大利亚电讯要看3G(第三代移动通信)牌照发给哪家运营商,谁来做相关的3G业务,再决定战略性的合作。西班牙运营商Telefonica、新加坡电信、英国沃达丰相关负责人也表达了同样的态度。 相似文献
8.
S. H. Anastasiadis J. K. Chen J. T. Koberstein J. E. Sohn J. A. Emerson 《Polymer Engineering and Science》1986,26(20):1410-1418
The interfacial tensions of immiscible binary blends of poly(dimethyl siloxane) (PDMS) and polybutadiene (PBD) have been determined as a function of molecular weight and temperature. The technique employed for these measurements takes advantage of recent advances in the determination and analysis of pendant fluid drop profiles. The experimental data are compared to the predictions of square gradient theories and theories based on the diffusion equation approach as developed by Helfand and co-workers. Qualitative agreement is obtained with both types of theory when the Flory-Huggins interaction parameter is taken to be comprised of two terms: a temperature independent term of entropic origin; and an enthalpic term that is inversely dependent upon the temperature. 相似文献
9.
Chris Parnin Christian Bird Emerson Murphy-Hill 《Empirical Software Engineering》2013,18(6):1047-1089
Support for generic programming was added to the Java language in 2004, representing perhaps the most significant change to one of the most widely used programming languages today. Researchers and language designers anticipated this addition would relieve many long-standing problems plaguing developers, but surprisingly, no one has yet measured how generics have been adopted and used in practice. In this paper, we report on the first empirical investigation into how Java generics have been integrated into open source software by automatically mining the history of 40 popular open source Java programs, traversing more than 650 million lines of code in the process. We evaluate five hypotheses and research questions about how Java developers use generics. For example, our results suggest that generics sometimes reduce the number of type casts and that generics are usually adopted by a single champion in a project, rather than all committers. We also offer insights into why some features may be adopted sooner and others features may be held back. 相似文献
10.