Gravity influences the position of the dorsoventral axis in medaka fish embryos (Oryzias latipes)

OBJECTIVE: To evaluate the use of a selective dopamine-1 agonist (fenoldopam) to provide selective splanchnic vasodilatation during sustained hypotensive endotoxaemia in sheep. DESIGN: Randomised, controlled, experimental study. SETTING: Animal research laboratory. SUBJECTS: 12 adult instrumented, midazolam-sedated sheep. INTERVENTIONS: The animals were randomised to receive a 20-min continuous infusion of dopamine (10 microg x kg(-1) x min(-1), fenoldopam (10 microg x kg(-1) x min(-1) and noradrenaline (1 microg x kg(-1) x min(-1)) under control conditions and 12 h after endotoxaemia was induced by a continuous infusion of Escherichia coli endotoxin producing a stable hyperdynamic state simulating human septic shock. This drug dosage was selected to produce a 25-30% increase in cardiac output by all three drugs during control conditions. MEASUREMENTS AND RESULTS: Systemic and splanchnic haemodynamic data were continuously obtained and systemic and splanchnic oxygen delivery (DO2) and consumption (VO2) were calculated. Hyperdynamic hypotensive endotoxaemia did not modify the splanchnic and renal reduction in DO2 and the vasoconstrictive reactivity to noradrenaline observed during control conditions. In contrast, endotoxaemia abolished the fenoldopam and dopamine-induced increase in splanchnic DO2 (especially in the coeliac trunk) observed during control conditions. CONCLUSIONS: During sustained hyperdynamic endotoxaemia, the dopaminergic-induced selective increase in coeliac trunk blood flow is abolished, most probably because of an already maximally vasodilated splanchnic circulation which prevented dopamine or fenoldopam to vasodilate this area further. Contrary to common belief, selective dopamine-1 agonist administration under these conditions may therefore not be beneficial to the splanchnic organs, though it improves whole body DO2 and VO2.     

Continuous online monitoring of ionic dialysance allows modification of delivered hemodialysis treatment time

Considerable intrinsic intrapatient variability influences the actual delivery of Kt/V. The aim of this study is to examine the feasibility of using continuous online assessment of ionic dialysance measurements (Kt/V(ID)) to allow dialysis sessions to be altered on an individual basis. Ten well-established chronic hemodialysis (HD) patients without significant residual renal function were studied (mean age 65+/-4.3 [38-81] years, mean length of time on dialysis 66+/-18 [14-189] months). These patients had all been receiving thrice-weekly 4-hr dialysis using Integra dialysis monitors. Dialysis monitors were equipped with Diascan modules permitting measurement of Kt/V(ID). Predicted treatment time required to achieve a Kt/V(ID) > or = 1.1 (equivalent to a urea-based method of 1.2) was calculated from the delivered Kt/V(ID) at 60 and 120 min. Treatment time was reprogrammed at 2 hr (ensuring all planned ultrafiltration would be accommodated into the new modified session duration). Owing to practical issues, and to avoid excessively short dialysis times, these changes were censored at no more than+/-10% of the usual 240-min treatment time (210-265 min). Data were collected from a total of 50 dialysis sessions. Almost all sessions (47/50) required modification of the standard treatment time: 13/50 sessions were lengthened and 34/50 shortened (mean length of session 232.2+/-2.5 [210-265] min). A Kt/V(ID) of > or = 1.1 was achieved in 39/50 sessions. The difference in mean urea-based Kt/V poststudy (1.3+/-0.05 [1.1-1.6]) and mean achieved Kt/V(ID) (1.16+/-0.02 [0.7-1.37]) was significant (p = 0.002). The use of individualized variable dialysis treatment time using online ionic dialysance measurements of Kt/V(ID) appears both practicable and effective at ensuring consistently delivered adequate dialysis.     

Semiconductor saturable absorber mirrors (SESAM's) for femtosecondto nanosecond pulse generation in solid-state lasers

Intracavity semiconductor saturable absorber mirrors (SESAM's) offer unique and exciting possibilities for passively pulsed solid-state laser systems, extending from Q-switched pulses in the nanosecond and picosecond regime to mode-locked pulses from 10's of picoseconds to sub-10 fs. This paper reviews the design requirements of SESAM's for stable pulse generation in both the mode-locked and Q-switched regime. The combination of device structure and material parameters for SESAM's provide sufficient design freedom to choose key parameters such as recovery time, saturation intensity, and saturation fluence, in a compact structure with low insertion loss. We have been able to demonstrate, for example, passive modelocking (with no Q-switching) using an intracavity saturable absorber in solid-state lasers with long upper state lifetimes (e.g., 1-μm neodymium transitions), Kerr lens modelocking assisted with pulsewidths as short as 6.5 fs from a Ti:sapphire laser-the shortest pulses ever produced directly out of a laser without any external pulse compression, and passive Q-switching with pulses as short as 56 ps-the shortest pulses ever produced directly from a Q-switched solid-state laser. Diode-pumping of such lasers is leading to practical, real-world ultrafast sources, and we will review results on diode-pumped Cr:LiSAF, Nd:glass, Yb:YAG, Nd:YAG, Nd:YLF, Nd:LSB, and Nd:YVO₄     

Cerenkov-type second-harmonic generation in KNbO3channel waveguides

Cerenkov-type second-harmonic generation using KNbO₃ channel waveguides produced by MeV He⁺-ion implantation is presented from the viewpoint of device design. We derive the Cerenkov phase-matching condition for multimode waveguides and utilize Cerenkov-angle analysis as a tool for contact-free measurement of the effective indexes of guided modes of ion-implanted KNbO₃ channel waveguides at a wavelength of 860 nm. The measured mode indexes are in full agreement with calculations based on the effective-index method and the refractive index-depth profiles of ion-implanted KNbO₃ waveguides. The efficiency of Cerenkov-type second-harmonic generation is modeled using analytical approximations of the field distributions of the fundamental and the Cerenkov-radiation modes in embedded-channel waveguides. The acceptance width for Cerenkov-type frequency doubling in these ion-implanted waveguides is about one order of magnitude wider than for noncritical phase-matched second-harmonic generation in bulk KNbO₃ crystals. Based on the theoretical simulations, guidelines for optimum device design are given, and the possibility to increase the ultimate conversion efficiency to about 30% W^-1 cm^-1 through lateral-resonance enhancement of the second-harmonic field in KNbO₃ channel waveguides is demonstrated     

Second-harmonic generation in potassium niobate waveguides

We report on our progress in the formation of waveguides in potassium niobate (KNbO₃) using techniques such as ion implantation and ion sputtering. Different methods for the structuring of channel waveguides are presented, and their advantages and disadvantages are discussed in terms of their optical and nonlinear optical properties. The excellent power-handling capability of KNbO₃ waveguides is compared to other waveguide materials, and we highlight the influence of postimplantation annealing and repoling on the waveguide attenuation and the nonlinear optical coefficient. We also review recent results on second-harmonic generation in KNbO₃ waveguides focusing on blue light generation     

The induction of transplantation tolerance by intrathymic (i.t.) delivery of alloantigen: a critical relationship between i.t. deletion, thymic export of new T cells and the timing of transplantation

Intrathymic (i.t.) injection of donor alloantigens has proved to be an effective strategy for the induction of tolerance. However, the mechanisms by which tolerance is induced and maintained after transplantation remain unclear. In this report we show that tolerance to donor cardiac allografts can be induced across a MHC class I difference by i.t. injection of donor splenocytes and transient T cell depletion. Furthermore, using H-2K(b)-specific TCR transgenic mice (BM3), we demonstrate that prolonged deletion of donor-reactive thymocytes was essential to induce tolerance by i.t. injection and this was dependent upon donor cells persisting in the thymus. Examination of the kinetics of thymic export following i.t. injection revealed that prolonged deletion of thymocytes was required to delay export of new T cells to the periphery until the time of transplantation. Importantly, after transplantation donor cell persistence in the thymus and i.t. deletion were no longer necessary to maintain tolerance. The graft itself or cells from the graft was responsible for maintaining tolerance at this stage. These findings reveal that multiple mechanisms are responsible for the induction and maintenance phases of tolerance to alloantigens in vivo after i.t. delivery, and that a complex inter-relationship between donor cell persistence in the thymus, i.t. deletion, thymic export of T cells and the timing of transplantation is involved.     

A fast stochastic solution method for the Blade Element Momentum equations for long‐term load assessment

Unsteady power output and long‐term loads (extreme and fatigue) drive wind turbine design. However, these loads are difficult to include in optimization loops and are typically only assessed in a post‐optimization load analysis or via reduced‐order methods. Both alternatives yield suboptimal results. The reason for this difficulty lays in the deterministic approaches to long‐term loads assessment. To model the statistics of lifetime loads they require the analysis of many unsteady load cases, generated from many different random seeds—a computationally expensive procedure. In this paper, we present an alternative: a stochastic solution for the unsteady aerodynamic loads based on a projection of the unsteady Blade Element Momentum (BEM) equations onto a stochastic space spanned by chaos exponentials. This approach is similar to the increasingly popular polynomial chaos expansion, but with 2 major differences. First, the BEM equations constitute a random process, varying in time, while previous polynomial chaos expansion methods were concerned with random parameters (ie, random but constant in time or initial values). Second, a new, more efficient basis (the exponential chaos) is used. This new stochastic method enables us to obtain unsteady long‐term loads much faster, enabling unsteady loads to become accessible inside wind turbine optimization loops. In this paper we derive the stochastic BEM solution and present the most relevant results showing the accuracy of the new method.     

Knowledge environments representing molecular entities for the virtual physiological human

In essence, the virtual physiological human (VPH) is a multiscale representation of human physiology spanning from the molecular level via cellular processes and multicellular organization of tissues to complex organ function. The different scales of the VPH deal with different entities, relationships and processes, and in consequence the models used to describe and simulate biological functions vary significantly. Here, we describe methods and strategies to generate knowledge environments representing molecular entities that can be used for modelling the molecular scale of the VPH. Our strategy to generate knowledge environments representing molecular entities is based on the combination of information extraction from scientific text and the integration of information from biomolecular databases. We introduce @neuLink, a first prototype of an automatically generated, disease-specific knowledge environment combining biomolecular, chemical, genetic and medical information. Finally, we provide a perspective for the future implementation and use of knowledge environments representing molecular entities for the VPH.     

Sex differences in benzodiazepine binding in the frontal cortex and amygdala of the rat 24 hours after restraint stress

Gravidic macromastia is a condition characterized by an excessive and generalized enlargement of the breasts which occurs during pregnancy or shortly after pregnancy. It is a rare condition whose etiology is unknown. Most Authors describe it as a pathology which is influenced by a disturbed play of hormones of hypersensitivity of mammary tissue to normal hormone stimulation. A therapeutic approach is discussed with reference to the literature.