Minocycline Loaded Hybrid Composites Nanoparticles for Mesenchymal Stem Cells Differentiation into Osteogenesis

Bone transplants are used to treat fractures and increase new tissue development in bone tissue engineering. Grafting of massive implantations showing slow curing rate and results in cell death for poor vascularization. The potentials of biocomposite scaffolds to mimic extracellular matrix (ECM) and including new biomaterials could produce a better substitute for new bone tissue formation. A purpose of this study is to analyze polycaprolactone/silk fibroin/hyaluronic acid/minocycline hydrochloride (PCL/SF/HA/MH) nanoparticles initiate human mesenchymal stem cells (MSCs) proliferation and differentiation into osteogenesis. Electrospraying technique was used to develop PCL, PCL/SF, PCL/SF/HA and PCL/SF/HA/MH hybrid biocomposite nanoparticles and characterization was analyzed by field emission scanning electron microscope (FESEM), contact angle and Fourier transform infrared spectroscopy (FT-IR). The obtained results proved that the particle diameter and water contact angle obtained around 0.54 ± 0.12 to 3.2 ± 0.18 µm and 43.93 ± 10.8° to 133.1 ± 12.4° respectively. The cell proliferation and cell-nanoparticle interactions analyzed using (3-(4,5-dimethyl thiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt) MTS assay (Promega, Madison, WI, USA), FESEM for cell morphology and 5-Chloromethylfluorescein diacetate (CMFDA) dye for imaging live cells. Osteogenic differentiation was proved by expression of osteocalcin, alkaline phosphatase activity (ALP) and mineralization was confirmed by using alizarin red (ARS). The quantity of cells was considerably increased in PCL/SF/HA/MH nanoparticles when compare to all other biocomposite nanoparticles and the cell interaction was observed more on PCL/SF/HA/MH nanoparticles. The electrosprayed PCL/SF/HA/MH biocomposite nanoparticle significantly initiated increased cell proliferation, osteogenic differentiation and mineralization, which provide huge potential for bone tissue engineering.     

Fabrication and characterization of high flux poly(vinylidene fluoride) electrospun nanofibrous membrane using amphiphilic polyethylene-block-poly(ethylene glycol) copolymer

Electrospun nanofiber membranes (ENM) made of polymers such as polysulfone and poly(vinylidene fluoride) (PVDF) have a much higher contact angle (CA) and also more hydrophobic when compared to the virgin polymers. For water treatment applications, membranes with hydrophilic nature are highly desirable in order to achieve high flux and less fouling potentials. Hence, in the present study, highly hydrophilic electrospun nanofiber membranes (ENMs) were prepared by blending PVDF polymer with amphiphilic polyethylene-block-poly (ethylene glycol) (PE-b-PEG) copolymer. Resulting amphiphilic ENMs were highly porous (77%–92%) and the breaking elongation of 140% with a young's modulus of 2.55 MPa was observed. When compared with the control PVDF membrane, PE-b-PEG blended ENMs revealed higher water permeation flux owing to the enrichment of the hydrophilic PEG segments at the membrane surface, which was confirmed by using X-ray photoelectric spectroscopy and Energy-dispersive spectroscopy measurements. When compared to the phase inversion process (CA of 97.3°) blended ENM had CA of 0°, which indicates that besides hydrophilic block copolymer segments, the nature of membrane formation also contributes its role in influencing the hydrophilicity of the membrane. This improved hydrophilicity in combination with larger pore sizes of the PVDF/ PE-b-PEG membranes have contributed to enhancement of pure water flux, protein solution permeability and water flux recovery, which can be applied potentially for water treatment applications.     

Spectrum Resource Utilization and Security Enhancement in Multi-domain Elastic Optic Networks (MD-EON) Using Memetic Algorithm

Wireless Personal Communications - Routing and Spectrum utilization is a challenging approach when optical signal is used in data transfer. The underlying bandwidth requirements in supporting the...     

Improved segmentation and change detection of multi-spectral satellite imagery using graph cut based clustering and multiclass SVM

Multimedia Tools and Applications - The Satellite image analysis automatically looks over an image to attain valuable information such as land cover classification and change detection from it....     

Investigation on the working performance of partitionable-space enhanced coagulation reactor

In this study, the phosphorous removal efficiency of partitionable-space enhanced coagulation (PEC) technology has been investigated. A series of continuous experiments was conducted to find out the influence of operating parameters. With increase in the elapsed treatment time, the phosphorus removal rates improved progressively during 1.5–3 h and the reactor reached a steady state after 4 h. This improved performance is mainly attributed to the partitionable-space and “flocculation filter” in the PEC reactor which enhanced the coagulation process. The flocs formed exhibited excellent settling characteristics by quickly settling out within the first 15 min.     

Sulfonated Poly (Ether Ether Ketone) / Barium Strontium Titanium Oxide Polymer Nanocomposite Membranes for Fuel Cell Applications

ABSTRACT

Polymer nanocomposite membranes based on sulfonated poly (ether ether ketone) (SPEEK) and barium strontium titanium oxide (BSTO) have been prepared by solution casting technique. The SPEEK was obtained by direct sulfonation of poly (ether ether ketone) using concentrated sulfuric acid. The degree of sulfonation of SPEEK was determined by ¹H NMR spectroscopy. The composite membranes showed higher water uptake capacity when compared to SPEEK. The ion-exchange capacity of the composite membranes was found in the range between 1.48 and 1.62 meq. g^?1. The thermal stability of composite membrane was found to be good enough to use in fuel cell.     

Mobile Malicious Node Detection Using Mobile Agent in Cluster-Based Wireless Sensor Networks

Many application domains require that sensor node to be deployed in harsh or hostile environments, such as active volcano area tracking endangered species, etc. making these nodes more prone to failures. The most challenging problem is monitoring the illegal movement within the sensor networks. Attacker prefers mobile malicious node because by making the diversity of path intruder maximize his impact. The emerging technology of sensor network expected Intrusion detection technique for a dynamic environment. In this paper, a defective mechanism based on three-step negotiation is performed for identifying the mobile malicious node using the mobile agent. In many approaches, the multi-mobile agents are used to collect the data from all the sensor nodes after verification. But it is inefficient to verify all the sensor nodes (SNs) in the network, because of mobility, energy consumption, and high delay. In the proposed system this can be solved by grouping sensor nodes into clusters and a single mobile agent performs verification only with all the cluster heads instead of verifying all the SNs. The simulation result shows the proposed system shows a better result than the existing system.