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1.
A logical design that describes the overall structure of proteins, together with a more detailed design describing secondary and some supersecondary structures, has been constructed using the computer-aided software engineering (CASE) tool, Auto-mate. Auto-mate embodies the philosophy of the Structured Systems Analysis and Design Method (SSADM) which enables the logical design of computer systems. Our design will facilitate the building of large information systems, such as databases and knowledgebases in the field of protein structure, by the derivation of system requirements from our logical model prior to producing the final physical system. In addition, the study has highlighted the ease of employing SSADM as a formalism in which to conduct the transferral of concepts from an expert into a design for a knowledge-based system that can be implemented on a computer (the knowledge-engineering exercise). It has been demonstrated how SSADM techniques may be extended for the purpose of modelling the constituent Prolog rules. This facilitates the integration of the logical system design model with the derived knowledge-based system. 相似文献
2.
South Africa’s Reconstruction and Development Programme set ambitious goals for providing basic services to all, including housing and electrification. More efficient use of energy has the potential to socially and politically support these goals, particularly when it is targeted at low-income communities lacking adequate energy services. This paper examines the economics of energy efficiency for the urban poor from the perspective of society, utilities and poor consumers, using five example programmes. While the five energy efficiency programmes generally have significant economic and environmental benefits from a social perspective, they may not be as attractive to utilities and consumers. Also examined are the policy options for overcoming the significant barriers to energy efficiency, and ways government can bridge the gap between what is good for society and what is good for the electricity industry. 相似文献
3.
4.
Sugarcane can be very susceptible to damage by freezes. Freeze-deteriorated cane can cause problems in processing and sometimes leads to a factory shut-down. This study was undertaken during the 2000/2001 harvest season to assess the cold tolerance performance of six commercial sugarcane varieties and to establish new and more sensitive criteria to measure cold tolerance. Two varieties CP 70-321 and CP 79-318, with known cold tolerance, were planted in the study as controls. The other varieties included LHo 83-153, LCP 85-384, HoCP 85-845 and HoCP 91-555. Freezing temperatures occurred on 20 December 2000 when the min. field temperature was −4.4 °C, and again on 21 December, 30 December through 5 January 2001, 9–10 January and 20–21 January. The lowest field temperature recorded was −5.6 °C on 4 January. Freezing conditions prevailed for 8–15 h during each freeze incident. Stalks of all varieties were frozen to the ground following the initial freeze, with freeze cracks evident only after the 4 January freeze. For this study, samples were taken on the date of the first freeze, 20 December, and subsequently again at 7, 14, 22 and 30 days after the first freeze. Criteria used to measure overall stalk cold-tolerance included changes in pH, Brix, dextran (ASI-II method), sucrose, glucose, and fructose concentrations. Mannitol, ethanol and the oligosaccharides, palatinose, leucrose, iso-maltotriose and 1-kestose, were simultaneuously measured using IC-IPAD. Marked differences were observed in most criteria for all varieties, particularly 22 and 30 days after the first freeze. Mannitol was strongly correlated (r2=0.84) with dextran, confirming its use as an indicator for cane dextran deterioration. In comparison, ethanol was only weakly correlated (r2=0.55) with dextran and did not always predict cane dextran deterioration. Iso maltotriose was the most sensitive oligosaccharide indicator of freeze deterioration, although both leucrose and palatinose could be used to confirm whether severe dextran formation (>1500 ppm/Brix) has occurred in cane. Isomaltotriose was strongly correlated (r2=0.89) with dextran and pH (r2=−0.83); pH was also a strong indicator of both dextran (r2=−0.85) and mannitol (r2=−0.92) formation. Four of the varieties, CP 79-318, LCP 85-384, HoCP 85-845 and HoCP 91-555, were shown to be susceptible to other sources of microbial and enzymic deterioration as well as dextran deterioration from Leuconostoc bacteria, especially 30 days after the first freeze. This was indicated by increased glucose/fructose ratios, ethanol formation, changes in 1-kestose concentration, and further sucrose losses. 相似文献
5.
Gillian Allard 《AI & Society》2003,17(1):12-24
The power of some new entrants to the music industry derives from their position as brokers in computer-mediated environments.
Brokers act instrumentally to exploit their position within a network which, in turn, depends on their ability to build and
sustain links (and, in computer-mediated environments, hyperlinks). Bricolage in computer-mediated entrepreneurship refers
to the intuitive manipulation of resources in order to achieve (perhaps tacit) goals. Without careful stewardship of the new
intellectual wealth thus created, bricolage may profit neither the individual nor the wider community.
Accepted 14 March 2002 相似文献
6.
Christopher L Brown Gillian Bushell Michael W Whitehouse DS Agrawal SG Tupe KM Paknikar Edward RT Tiekink 《Gold bulletin》2007,40(3):245-250
Nanosized gold particles (27 +/− 3 nm) have been proven to be effective in ameliorating the symptoms of mycobacterial-, collagen-
and pristane-induced arthritis in rat models. This contrasts with the drug sodium aurothiomalate that was only effective against
mycobacterial-induced arthritis but not to the same extent as Au0. Gold in the traditional Indian Ayurvedic medicine,Swarna bhasma (gold ash), has been characterized as globular particles of gold with an average size of 56–57 nm. 相似文献
7.
The behavioral and physiological effects of plant allelochemicals have been difficult to demonstrate; it is not often clear whether the compounds are deterrent, toxic, or both. In this study, we compared the qualitative and quantitative effects of several iridoid glycosides on a generalist lepidopteran herbivore,Spodoptera eridania (Noctuidae). Larval growth and survivorship and larval preference or avoidance were measured on artificial diets containing different iridoid glycosides at different concentrations. We also tested the toxicity/deterrence of these compounds. We found that iridoid glycosides retarded larval growth significantly at relatively low concentrations and that they were usually avoided in preference tests. The toxicity/ deterrence test did not always reflect the results of these other tests. The merits of using a variety of methods for determining deterrence and/or toxicity of plant allelochemicals are discussed. 相似文献
8.
Sequence and structure of VH domain from naturally occurring camel heavy chain immunoglobulins lacking light chains 总被引:9,自引:0,他引:9
Muyldermans S.; Atarhouch T.; Saldanha J.; Barbosa J.A.R.G.; Hamers R. 《Protein engineering, design & selection : PEDS》1994,7(9):1129-1135
We cloned 17 different PCR fragments encoding VH genes of camel(Camelus dromedarius). These clones were derived from the camelheavy chain immunoglobulins lacking the light chain counterpartof normal immunoglobulins. Insight into the camel VH sequencesand structure may help the development of single domain antibodies.The most remarkable difference in the camel VH, consistent withthe absence of the VL interaction, is the substitution of theconserved Leu45 by an Arg or Cys. Another noteworthy substitutionis the Leu11 to Ser. This amino acid normally interacts withthe CH1 domain, a domain missing in the camel heavy chain immunoglobulins.The nature of these substitutions agrees with the increasedsolubility behavior of an isolated camel VH domain. The VH domainsof the camels are also characterized by a long CDR3, possiblycompensating for the absence of the VL contacts with the antigen.The CDR3 lacks the salt bridge between Arg94 and Asp101. However,the frequent occurrence of additional Cys residues in both theCDR1 and CDR3 might lead to the formation of a second internaldisulfide bridge, thereby stabilizing the CDR structure as inthe DAW antibody. Within CDRs of the camel VH domains we observea broad size distribution and a different amino acid patterncompared with the mouse or human VH. Therefore the camel hypervariableregions might adopt structures which differ substantially fromthe known canonical structures, thereby increasing the repertoireof the camel antigen binding sites within a VH. 相似文献
9.
Response of generalist and specialist insects to qualitative allelochemical variation 总被引:7,自引:0,他引:7
We examined the effects of a set of four biosynthetically related iridoid glycosides, aucubin, catalpol, loganin, and asperuloside, on larvae of a generalist,Lymantria dispar (Lymantriidae), the gypsy moth, and an adapted specialist, the buckeye,Junonia coenia (Nymphalidae). In general,L. dispar grew and survived significantly less well on artificial diets containing iridoid glycoside, compared to a control diet without iridoid glycosides. In choice tests, previous exposure to a diet containing iridoid glycosides caused larvae subsequently to prefer iridoid glycoside-containing diets even though they were detrimental to growth and survival. In contrast,J coenia larvae grew and survived better on diets with aucubin and catalpol, the two iridoid glycosides found in the host plantPlantago lanceolata (Plantaginaceae), than on diets with no iridoid glycoside or with loganin and asperuloside. The results of choice tests of diets with and without iridoid glycosides and between diets with different iridoid glycosides reflected these differences as well. These results are discussed in terms of (1) differences between generalists and specialists in their response to qualitative variation in plant allelochemical content, (2) the induction of feeding preferences, and (3) the evolution of qualitative allelochemical variation as a plant defense. 相似文献
10.
8月中旬当美国大型投资机构Sentinel停止投资者抽离资金时,它的理由是市场出现了“恐慌”。毫无疑问:近日全球市场剧烈震荡,导致金融界某些领域的危机感日益加剧。[第一段] 相似文献