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Mengyi Chen Xiaoyang Zhu Xiaojuan Liu Caiyu Wu Canye Yu Guojian Hu Lin Chen Riyuan Chen Mondher Bouzayen Mohammed Zouine Yanwei Hao 《International journal of molecular sciences》2021,22(7)
Auxin response factors (ARFs) play important roles in various plant physiological processes; however, knowledge of the exact role of ARFs in plant responses to water deficit is limited. In this study, SlARF4, a member of the ARF family, was functionally characterized under water deficit. Real-time fluorescence quantitative polymerase chain reaction (PCR) and β-glucuronidase (GUS) staining showed that water deficit and abscisic acid (ABA) treatment reduced the expression of SlARF4. SlARF4 was expressed in the vascular bundles and guard cells of tomato stomata. Loss of function of SlARF4 (arf4) by using Clustered Regularly Interspaced Short Palindromic Repeats/Cas 9 (CRISPR/Cas 9) technology enhanced plant resistance to water stress and rehydration ability. The arf4 mutant plants exhibited curly leaves and a thick stem. Malondialdehyde content was significantly lower in arf4 mutants than in wildtype plants under water stress; furthermore, arf4 mutants showed higher content of antioxidant substances, superoxide dismutase, actual photochemical efficiency of photosystem II (PSII), and catalase activities. Stomatal and vascular bundle morphology was changed in arf4 mutants. We identified 628 differentially expressed genes specifically expressed under water deficit in arf4 mutants; six of these genes, including ABA signaling pathway-related genes, were differentially expressed between the wildtype and arf4 mutants under water deficit and unlimited water supply. Auxin responsive element (AuxRE) elements were found in these genes’ promoters indicating that SlARF4 participates in ABA signaling pathways by regulating the expression of SlABI5/ABF and SCL3, thereby influencing stomatal morphology and vascular bundle development and ultimately improving plant resistance to water deficit. 相似文献
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李国建 《锻压装备与制造技术》2003,38(4):23-24
介绍了快速制动双绕组电动机的结构原理、性能特点,选用该电动机应注意的一些问题及其在压力机上的应用。 相似文献
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作为微波真空电子器件的常用材料之一,无氧铜材料的蒸发特性会对微波真空电子器件的电性能产生影响.该文利用超高真空测试设备,研究了处理工艺对无氧铜材料的蒸发性能的影响,采用X射线测厚仪测试了蒸发的铜膜厚度,用扫描电镜(SEM)观测了无氧铜材料的表面形貌.结果表明表面宏观形貌粗糙度对无氧铜材料的蒸发性能影响不大,但处理工艺对蒸发性能影响很大;无氧铜材料经过酸洗后,会大大增加蒸发量;无氧铜材料经过烧氢处理,可降低蒸发量,而经过去油清洗并烧氢处理的无氧铜的蒸发量极低.对无氧铜材料进行了表面分析,发现无氧铜材料的真空蒸发性能与材料的表面形貌状态有关,当表面微观形貌比较光滑、无孔洞等缺陷时,无氧铜材料的真空蒸发量就少. 相似文献
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The natural calculation region in fluid dynamics involves complex boundaries. When using the Cartesian grid to approximate complex boundaries, two difficulties develop: the boundary zigzag effect and disagreement of direction of grid line and velocity. The multidimensional upwind scheme of the diagonal Cartesian method (DCM), using both Cartesian grid lines and diagonal lines segments, is presented in this paper to simulate the complex boundaries of the multiple-layer quasi 3D model equations. The DCM improves the simulation accuracy for the boundaries and calculation time increases only slightly compared to the Cartesian method. In order to verify the new scheme, a test case is presented which rotates the cavity flow at 45° to compare the numerical calculation results at different Reynolds numbers. The test case shows that the scheme is accurate and efficient in improving the simulation results. Then the three-dimensional advection-diffusion processes in the tidal water of the Hongyanhe Power Plant are simulated using this model. Numerical results show that the scheme is not only efficient on an experiment basis, but also efficient and reliable when applied to a large scale natural water area. 相似文献
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针对多传感信息耦合问题,基于IEEE1451标准,提出了一种通用的多传感信息自校正模型。模型根据传感元件输出信号额定特征,从信号幅度、变化趋势等方面,实现对传感器的故障判断和自免疫处理;然后基于多传感信息插值解耦数学原理,建立了基于特定TEDS格式的校正引擎,通过将标定点数据以矩阵表格形式输入TEDS中来实现解耦校正。试验研究表明:通过TEDS解耦校正引擎,可实现对多传感耦合信息的良好校正补偿,提高检测准确度,在解耦校正的前端引入传感元件的自评估技术,可有效提高智能传感器自校正的可靠性。 相似文献
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Guojian Wang Xiumei Han Mingjun Song Guofu Wang Xifa Long 《Materials Letters》2006,60(29-30):3710-3713
(Ba0.32Sr0.68)5Nb4O15 crystal with sizes of Ø 17 × 35 mm was grown successfully by Czochralski technique method. The thermal anisotropy was discussed. The principal coefficients of thermal expansion along (100), (010), (001) directions were precisely measured to be 1.308 × 10− 5, 1.288 × 10− 5, 1.478 × 10− 5 K− 1, respectively. Its optical transparency range has been measured and found to span from 323 to 5500 nm. The bands present in the IR spectra were identified and assigned to the corresponding vibration modes of NbO6 anions. 相似文献