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排序方式: 共有234条查询结果,搜索用时 15 毫秒
1.
Bernette Maria Oosterlaken Heiner Friedrich Gijsbertus de With 《Microscopy research and technique》2022,85(1):412-417
Transmission electron microscopy (TEM) is an important analysis technique to visualize (bio)macromolecules and their assemblies, including collagen fibers. Many protocols for TEM sample preparation of collagen involve one or more washing steps to remove excess salts from the dispersion that could hamper analysis when dried on a TEM grid. Such protocols are not standardized and washing times as well as washing solvents vary from procedure to procedure, with each research group typically having their own protocol. Here, we investigate the influence of washing with water, ethanol, but also methanol and 2-propanol, for both mineralized and unmineralized collagen samples via a protocol based on centrifugation. Washing with water maintains the hydrated collagen structure and the characteristic banding pattern can be clearly observed. Conversely, washing with ethanol results in dehydration of the fibrils, often leading to aggregation of the fibers and a less obvious banding pattern, already within 1 min of ethanol exposure. As we show, this process is fully reversible. Similar observations were made for methanol and propanol. Based on these results, a standardized washing protocol for collagenous samples is proposed. 相似文献
2.
On the application of ion sensitive field-effect transistors for the investigation of corrosion phenomena Ion sensitive field-effect transistors (ISFET) are chemical sensors based on semiconductor technology. They exhibit small dimensions, a plane measuring surface and have short response times. Thus, they are very suitable for the determination of local changes of the pH value at electrochemical corrosion studies. The described in this paper experimental set-up allows in situ measurements of the pH value by means of ISFET sensors in amounts of liquids below 1 mm3; the active surface area of the material under test may be below 1 mm2. In model tests preferably changes of the pH value of dist. water due to the corrosion of steel St 38 have been investigated. After an initial short-time rise caused by oxygen reduction a decay of the pH value to pH = 3 ?4 was obseved. Furthermore, ISFET sensors have been used as a potential probe for electrochemical polarization measurements in low conductive media. It was shown, that the dynamic behaviour of potentiostatic control circuits may be influenced favourably by the low output resistance of the ISFET. 相似文献
3.
JP Heiner SS Kohles PA Manley R Vanderby MD Markel 《Canadian Metallurgical Quarterly》1997,(341):233-240
In a canine model, the fixation stability of a prosthesis and proximal bone graft composite were measured relative to the distal femur. One group had the prosthesis graft composite cemented into the distal femur. The second group had the prosthesis graft composite press fit into the distal femur for biologic ingrowth. Displacements of the proximal femoral grafts relative to the host bone in each group were measured after ex vivo (acute with graft) implantation and 4 months after implantation. A third group with no osteotomy (acute intact) simulated perfect graft to host bone union. Relative displacements representing 6 degrees freedom (translation and rotation) were calculated from the displacement values measured by 9 eddy current transducers. Measurements of displacement were used to test the hypothesis that distal press fit fixation equals distal cement fixation at 4 months after implantation. In all cases the measured translations and rotations of the graft to implant construct were small and of a magnitude that should encourage bone ingrowth (< 0.05 mm and < 0.1 degree, respectively). The stability of the press fit group at 4 months was not significantly different from the cemented group in axial and transverse displacement during axial and transverse loading, respectively. There was no difference in stabilities at 4 months between distal press fit and cemented fixation in hip replacements requiring a proximal femoral graft. 相似文献
4.
LG Lee SL Spurgeon CR Heiner SC Benson BB Rosenblum SM Menchen RJ Graham A Constantinescu KG Upadhya JM Cassel 《Canadian Metallurgical Quarterly》1997,25(14):2816-2822
We have synthesized a set of four energy transfer dyes and demonstrated their use in automated DNA sequencing. The donor dyes are the 5- or 6-carboxy isomers of 4'-aminomethylfluorescein and the acceptor dyes are a novel set of four 4,7-dichloro-substituted rhodamine dyes which have narrower emission spectra than the standard, unsubstituted rhodamines. A rigid amino acid linker, 4-aminomethylbenzoic acid, was used to separate the dyes. The brightness of each dye in an automated sequencing instrument equipped with a dual line argon ion laser (488 and 514 nm excitation) was 2-2.5 times greater than the standard dye-primers with a 2 times reduction in multicomponent noise. The overall improvement in signal-to-noise was 4- to 5-fold. The utility of the new dye set was demonstrated by sequencing of a BAC DNA with an 80 kb insert. Measurement of the extinction coefficients and the relative quantum yields of the dichlororhodamine components of the energy transfer dyes showed their values were reduced by 20-25% compared with the dichlororhodamine dyes alone. 相似文献
5.
6.
Membranes in chemical engineering . In the last 10 years membranes and membrane processes have evolved from a useful laboratory tool to an industrial product of significant technical and commercial impact. In some applications membrane processes have today not only replaced some conventional separation procedures in the chemical process industry, because they are often more economical and yield better quality products, but they have also successfully been utilized to solve mass separation problems where conventional procedures failed or are too expensive. This contribution discusses the state of the art of membranes and membrane processes as well as their major applications, with the main emphasis being placed on more recent developments. 相似文献
7.
Dr. Angelika Hüppe Dr. Katharina Dziubek Prof. Dr. Dr. Heiner Raspe 《Ethik in der Medizin》2014,26(3):211-224
Background
One of the elementary prerequisites for medical research on and with humans is the patients’ or probands’ informed consent. To ensure informed consent, study participants must be—among other things—provided with high-quality information. We developed criteria to assess and evaluate the quality of various written patient information material.Methods
Based on a catalogue addressing 117 single criteria, we assessed the quality of 128 randomly selected documents from study proposals submitted to the ethical committee of Luebeck University in 2006. Each criterion refers to one of six quality areas (such as “readability and comprehensibility” or “potential benefit and harm”).Results
The documents on average satisfied half of the criteria with a range from 20–76% for single items. The area with the highest quality score was “consent form” (64%), while “potential benefit and harm” (35%) was the lowest. Material from drug trials showed a significantly higher quality than that from other study types. Only 21 out of 117 criteria were met in more than 80% of all relevant documents.Conclusion
The study provides evidence for significant deficits in the information material from basic and clinical research projects presented to one academic research ethics committee. Researchers need support in developing and writing informed consent documents. Our set of criteria could be used to make them more sensitive to the various demands involved. 相似文献8.
Recent trends in manufacturing and health care move these two work systems closer together from a system ergonomics point of view. Individual treatment of products, especially patients, by specialists in a distributed environment demand information technology (IT)‐based support suitable for complex systems. IT‐based support of processes in complex systems is difficult due to the lack of standard processes. IT support also means to rethink processes to use efficiency potentials. Close cooperation of users and software developers is needed to increase the ergonomic quality of the system. Therefore, suitable tools are needed: UML is available as the standard industry modeling language, Zope/Plone as the quasi‐standard for content management systems, SimPy as an object‐oriented simulation tool for event‐triggered processes, and ACT‐R as a powerful cognitive architecture for simulation of human information processes. The integration of these tools enables system‐ergonomic support of processes in the complex work system as well as of the development and deployment process. It is the base of an integral system‐ergonomic approach for IT‐based process management. Knowledge gained during process analysis either enters models or leads to the extension and adaptation of the tool chain. The models serve as basis for discussion among system ergonomists, programmers, and specialists from the work system. Further, they are understood by simulation and process support tools. Transcoding efforts between humans with different professional backgrounds and machines are reduced, and the flexibility demanded by complex systems is met. © 2008 Wiley Periodicals, Inc. 相似文献
9.
The majority of approaches to activity recognition in sensor environments are either based on manually constructed rules for recognizing activities or lack the ability to incorporate complex temporal dependencies. Furthermore, in many cases, the rather unrealistic assumption is made that the subject carries out only one activity at a time. In this paper, we describe the use of Markov logic as a declarative framework for recognizing interleaved and concurrent activities incorporating both input from pervasive lightweight sensor technology and common-sense background knowledge. In particular, we assess its ability to learn statistical-temporal models from training data and to combine these models with background knowledge to improve the overall recognition accuracy. We also show the viability and the benefit of exploiting both qualitative and quantitative temporal relationships like the duration of the activities and their temporal order. To this end, we propose two Markov logic formulations for inferring the foreground activity as well as each activities’ start and end times. We evaluate the approach on an established dataset where it outperforms state-of-the-art algorithms for activity recognition. 相似文献
10.
Bubb H 《Applied ergonomics》2006,37(4):401-407
In this article, it is shown that human work can be understood as a process of creating order, and that order can be seen as a form of information. Since information can be considered as negative entropy, work is associated with energy consumption. Therefore, it is important to investigate the nature of human necessities in more detail in order to meet the desire for comfort through the efficient application of energy. Temporary increases of information cause accelerated increases in entropy. This explains the appearance of living organisms, and the historic development of increasingly complex technology. Through technical progress, repetitive human work is being replaced by automation, so that primarily creative work remains. Now the question arises of how much creative work a human can manage. In addition, one goal of automation should be the reduction of human errors, but in doing so, an optimal balance should be found between supporting the operator both during normal procedures and during unforeseen circumstances. 相似文献