This paper presents a microfluidic device for magnetophoretic separation of red blood cells from blood under continuous flow.
The separation method consists of continuous flow of a blood sample (diluted in PBS) through a microfluidic channel which
presents on the bottom “dots” of ferromagnetic layer. By applying a magnetic field perpendicular on the flowing direction,
the ferromagnetic “dots” generate a gradient of magnetic field which amplifies the magnetic force. As a result, the red blood
cells are captured on the bottom of the microfluidic channel while the rest of the blood is collected at the outlet. Experimental
results show that an average of 95% of red blood cells is trapped in the device. 相似文献
Polyphosphonates, polyphosphates, polyarylazophosphonates and polyarylazophosphates were synthesized by a new alternative to the classical phase transfer catalysis, respectively, the modified inverse phase transfer catalysis (IPTC) polycondensation of various phenylphosphonic (phosphoric) dichlorides (phenylphosphonic dichloride, phenylphosphoric dichloride, p-chlorophenylphosphoric dichloride) with bisphenols: bisphenol A, tetrabromobisphenol A, bis-(4-hydroxyphenyl)methane (bisphenol F), 4,4′-dihydroxyazobenzene. The polymers were characterized by infrared spectroscopy and magnetic resonance (1H-NMR, 31P-NMR, 13C-NMR) spectroscopy. Yields in the range of 63.5–85% and molecular weights (Mw) of ~2,000–8,200 g mol?1 were obtained. Polyphosphonates were stable up 210–270 °C and polyphosphates up 190–220 °C in air atmosphere. For a correct estimation of the thermal behavior of similar compounds, a kinetic analysis using a modified version of Non Parametric Kinetic method for representative polyphosphonate and polyphosphate was realized. The studies made on the hydrolytic degradation of the synthesized polyphosphates show that the most stable polymer under alkali-catalyzed degradation is the polyphosphate obtained by IPTC of phenylphosphoric dichloride and bisphenol A. 相似文献
Microsystem Technologies - Cell transplantation traditionally employs needles to inject donor cells into tissues to treat certain diseases. However, it is difficult for the current method to... 相似文献
A method for preparing organic–inorganic hybrids containing organophosphorus compounds, silica, and polyaniline (PANI) was
developed using sol–gel technique. This method allows the in situ synthesis of organic–inorganic hybrids by reacting tetraethoxysilane
(TEOS), aniline, initiator, organophosphorus compound in formic acid. The formic acid has multiple functions: as solvent and
acidic media for polymerization of aniline and reagent for sol–gel process. The use of an organophosphorus compound as coupling
agent and the introduction of a conductive polymer in silica matrix was investigated. 相似文献
The paper presents a dielectrophoretic chip, fully enclosed, with bulk silicon electrodes fabricated using wafer-to-wafer bonding techniques and packaged at the wafer level. The silicon electrodes, which are bonded to two glass dies, define in the same time the walls of the microfluidic channel. The device is fabricated from a silicon wafer that is bonded (at wafer level) anodically and using SU8 photoresist between two glass wafers. The first glass die includes drilled holes for inlet/outlet connections while the second glass die assure the electrical connections, through via holes and a metallization layer, between the silicon electrodes and a printing circuit board. 相似文献
This paper reports the highest etch depth of annealed Pyrex glass achieved by wet etching in highly concentrated HF solution, using a low stress chromium–gold with assistance of photoresist as masking layer. The strategies to achieve that are: increasing the etch rate of glass and simultaneously increasing the resistance of Cr/Au mask in the etchant. By annealing the Pyrex glass and using a highly concentrated HF acid, a high etch rate can be obtained. Furthermore, a method to achieve a good resistance of the Cr/Au masking layer in the etching solution is to control the residual stress and to increase the thickness of Au deposition up to 1 μm. In addition, the presence of a hard baked photoresist can improve the etching performance. As a result, a 500-μm thick Pyrex glass wafer was etched through.
Microfabricated systems provide an excellent platform for the culture of cells, and are an extremely useful tool for the investigation of cellular responses to various stimuli. Advantages offered over traditional methods include cost-effectiveness, controllability, low volume, high resolution, and sensitivity. Both biocompatible and bio-incompatible materials have been developed for use in these applications. Biocompatible materials such as PMMA or PLGA can be used directly for cell culture. However, for bio-incompatible materials such as silicon or PDMS, additional steps need to be taken to render these materials more suitable for cell adhesion and maintenance. This review describes multiple surface modification strategies to improve the biocompatibility of MEMS materials. Basic concepts of cell-biomaterial interactions, such as protein adsorption and cell adhesion are covered. Finally, the applications of these MEMS materials in Tissue Engineering are presented. 相似文献