A Taguchi design approach for the enhancement of a detergent-biocompatible alkaline thermostable protease production by Streptomyces mutabilis strain TN-X30

The ability of microorganisms to grow at high temperature, alkaline pH, and high salinity makes them an attractive target for enzyme-production with several industrial applications. One strain TN-X30 has been selected as protease producer and identified as Streptomyces mutabilis after a phenotypic and molecular study. Its production of protease was improved using Taguchi L27 design. The strategy was carried out to identify the optimum levels and the interaction of the screened factors. Following this step, maximum protease activity (10,895 U/ml) was achieved after 6-days of incubation. The TN-X30 protease activity had an optimum of pH and temperature of 10 and 65°C, respectively. Thermodynamic parameters at 60°C were enthalpy 14.26 kJ/mol, entropy −220 J/mol/K, and Gibbs free energy 90.53 kJ/mol. TN-X30 protease production displayed a 16-fold increase reaching 175,000 U/ml in a 100-L fermentor. Furthermore, the lyophilization in presence of sorbitol enhanced the stability of the TN-X30 protease which remained active at 75% after 24-months of storage. The lyophilized TN-X30 protease exhibited exceptional stability indexes in presence of some known commercialized detergent components as NEODOL® 25-7, Dehydol® LT 7, Na₂ CMC, Galaxy LAS, Galaxy LES 70, Galaxy 110, Galaxy CAPB Plus, and Sulfacid K. The lyophilized enzyme also displayed high stability with respect to both solid and liquid detergents. Finally, TN-X30 protease exhibited remarkable destaining of blood, egg, and chocolate stained cloth pieces. These findings may promote TN-X30 protease for use as bioadditive in detergent formulation, thereby reducing environmental chemical threat.     

A model-driven development approach for context-aware systems

The widespread usage of various types of computer devices with different platform characteristics created a need for new methods and tools to support the development of context-aware applications capable of dynamically adapting themselves to context changes. In this paper, we present a new model-based approach that addresses the development of context-aware applications from both the theoretical and practical perspectives and that supports all development phases of context-aware systems. On the one hand, we describe how our approach is applied to dynamically capture, observe the change of the context and notify the system at runtime. On the other hand, we show how our approach is used by programmers to develop a context- aware application.     

On the Spectral Efficiency of Energy Constrained Short-Range Communicating Systems

Binary offset carrier modulation is applied in several signals of the global navigation satellite system (GNSS). It enhanced the robustness of GNSS against the impact of multipath and make the compatibility of GNSS better. However, it has the main drawback of potentially giving biased measurements in acquisition and tracking. The technique we proposed is based on linear fitting with multi-correlator to accomplish unambiguous tracking. The coefficient of each correlator was deduced in this paper. The theoretical analysis and simulation results indicate that the proposed method can performs well on multipath and thermal noise.     

Single and multi-objective shape optimization of streamlined bridge decks

Structural and Multidisciplinary Optimization - Civil engineers focus on developing an optimum design that is cost-effective without compromising the performance. Experiences from optimizing...     

Kenaf fibre-reinforced polyester composites: flexural characterization and statistical analysis

This paper deals with the effect of the chemical treatment, fibre ratio and fibre reinforcement structure on the flexural properties of kenaf-polyester composites. Composites were made from an unsaturated polyester matrix reinforced with an alkali-treated and virgin kenaf fibres in a loose fibres and nonwovens. Results reveal that alkali treatment improves the flexural properties of composites expect elongation. The same result was obtained when using a nonwoven structure us reinforcement. The best flexural properties were observed for 11.1% fibre weight ratio with the nonwoven structure reinforce composite. The flexural strength and the flexural modulus were 69.5 MPa and 7.11 GPa, respectively, for this composite while it was 42.24 MPa and 3.61, respectively, for polyester samples (no fibre reinforcement). A statistical study was carried out in order to study the effect of the alkali treatment, reinforcement structure and the reinforcement weight ration on the composite properties. This study proved that the parameter with most impact on the measured properties is the fibre-to-matrix weight ratio. And also this study aims to determine the optimum parameters allowing maximising all measured properties and we found that when using a nonwoven structure made with chemically-treated fibre at 11.10% fibre weight ratio, is the optimum solution.     

New lab-scale device for nonwoven production: optimization of setting parameters

This paper studies the effectiveness of lab scale ‘air laid machine’ designed in the Laboratory of Textile Engineering in ISET of Ksar Hellal, Tunisia for production of nonwoven. Three different types of fibers were used: cotton, kenaf, and polyester. The uniformity of the nonwovens made in terms of thickness, weight per unit area (g/m²), air permeability, and mechanical properties were determined through their coefficient of variation. The method used to evaluate the effectiveness is the overload contour plot. This study demonstrated that ‘Airlaid machine’ is compatible with different types of fibers especially at high values of frequency and pressure.     

Fermentation of date palm juice by curdlan gum production from Rhizobium radiobacter ATCC 6466™: Purification, rheological and physico-chemical characterization

The present study was undertaken to investigate the possibility of using date palm juice byproducts for curdlan production by Rhizobium radiobacter ATCC 6466™ in batch experiments. A number of operational parameters, namely pH value, temperature range, inoculum ratio, agitation speed, carbon concentration, nitrogen source, and fermentation time, were investigated in terms of their optimal values for as well as individual and synergistic effects on curdlan production. The findings indicated that the strain exhibited a high ability to use the natural substrate under investigation. A curdlan production yield of 22.83 g/l was obtained in 500-ml agitated flasks (50 ml) when the strain was cultivated in the optimal medium (pH, 7; ammonium sulphate concentration, 2 g/l; date glucose juice concentration, 120 g/l) operating at 30 °C with an inoculum ratio of 5 ml/100 ml, an agitation speed of 180 rpm, and a fermentation period of 51 h. The purified date byproducts-curdlan (DBP-curdlan) had a molecular weight of 180 kDa, a linear structure composed exclusively of β-(1,3)-glucosidic linkages, a melting temperature (T_m) and glass transition temperature (T_g) of 1.24 and −3.55 °C, respectively. The average measured heights of its molecules were noted to fluctuate between 14.1 ± 0.07 and 211.73 ± 0.6 μm.