Monitoring und Diagnose von Hochspannungsschaltanlagen — Ein integraler Bestandteil der intelligenten Stationsleittechnik

After completing the integration of all bayoriented functions into the bay cubicle (modern substation automation) the next step brings intelligence even closer to the primary process. The introduction of new, intelligent sensors and actuators for all important measurements and setpoints is a prerequisite thereto. These sensors and actuators are linked together and to the bay level by means of a fieldbus. This permits a cost optimized, fully redundant acquisition and processing of all values as well as the plant wide diagnosis and monitoring of the substation.     

An 8-Gb/s simultaneous bidirectional link with on-die waveform capture

A full-duplex transceiver capable of 8-Gb/s data rates is implemented in 0.18-/spl mu/m CMOS. This equalized transceiver has been optimized for small area (329 /spl mu/m /spl times/ 395 /spl mu/m) and low power (158 mW) for point-to-point parallel links. Source-synchronous clocking and per-pin skew compensation eliminate coding bandwidth overhead and reduce latency, jitter, and complexity. This link is self-configuring through the use of automatic comparator offset trim and adaptive deskew. Comprehensive diagnostic capabilities have been integrated into the transceiver to provide link, interconnect, and circuit characterization without the use of external test equipment. With a resolution of 4 mV and 9 ps, these capabilities enable on-die eye diagram generation, equivalent time waveform capture, noise characterization, and jitter distribution measurements.     

A 3.6-Gb/s point-to-point heterogeneous-voltage-capable DRAM interface for capacity-scalable memory subsystems

We describe a DRAM interface operating at 3.6 Gb/s/pin implemented in 130-nm CMOS logic and 110-nm DRAM process technologies. It utilizes simultaneous bidirectional (SBD) signaling in a daisy-chained (repeated), point-to-point configuration to enable high performance scalable memory subsystems; and also provides direct attach capability for DRAMs to memory controllers or other logic devices. Source-synchronous strobes are used for data capture, minimizing strobe-to-data jitter. A low-jitter differential clock retimes the data at each DRAM on a per DIMM basis preventing jitter from accumulating in repeated data. The phase of this clock is adjusted on each DRAM to minimize the latency of the repeaters. 80 mW of total power is dissipated per DRAM I/O at 3.6 Gb/s. We present results from a system using both memory controller and DRAM repeater test chips.     

Enzyme-linked immunosorbent assay for measurement of JNK, ERK, and p38 kinase activities

A rapid enzyme-linked immunosorbent assay for the enzyme activity measurement of three well-known mitogen-activated protein (MAP) kinases, JNK2, ERK2, and p38 is described. The assay involves immobilization of the respective kinase substrates c-Jun, Elk1, or ATF2 on microtiter plates, addition of the kinase reaction mixture, and measurement of substrate phosphorylation using phospho-epitope-specific antibodies. This novel procedure represents a marked improvement to conventional radioactive MAP kinase assays in terms of quantification, precision, performance at physiological ATP concentration, high throughput, time consumption and amenability to automation. In addition to the standard solid phase assay using plastic-bound protein substrates, we developed an alternative solution phase protocol using soluble protein substrates. By comparing the results of the two assays, we found that MAP kinases retained much of their substrate specificity in the phosphorylation of immobilized protein substrates. Interestingly, we observed a strong preference of JNK2 and p38 for the phosphorylation of dimeric over monomeric substrates. We further characterized the kinase inhibitory activity of olomoucine, staurosporine, and SB 203580 for JNK2, ERK2, and p38. Taken together, this assay could assist in the biochemical characterization of MAP kinases and in identifying potent and specific inhibitors of these enzymes.     

A detailed experimental performance evaluation on TCP over UBR

The ATM service category UBR is intended for non-real-time applications that do not require guaranteed QoS commitments. With additional, relatively inexpensive control functions such as packet discard schemes, UBR could become a cost-effective alternative for the transmission of data traffic, offering a straightforward and flexible solution as opposed to nrt-VBR and GFR that applies stricter traffic specifications as well as ABR with its sophisticated and complex rate-control protocol. This paper presents the results obtained from a comprehensive set of experiments with TCP over UBR, comprising measurements taken on different protocol layers. The goal is to experimentally investigate the performance of UBR to carry TCP traffic, to evaluate the performance gain achievable by packet discard schemes and TCP parameter tuning, to study the influence of the TCP implementation, and in a final step, to relate the measurements to simulation results. This revised version was published online in August 2006 with corrections to the Cover Date.     

Mutant aspartate aminotransferase (K258H) without pyridoxal-5'-phosphate-binding lysine residue. Structural and catalytic properties

If the pyridoxal-phosphate-binding lysine residue 258 of aspartate aminotransferase is exchanged for a histidine residue, the enzyme retains partial catalytic competence [Ziak, M., Jaussi, R., Gehring, H. and Christen, P. (1990) Eur. J. Biochem. 187, 329-333]. The three-dimensional structures of the mutant enzymes of both chicken mitochondria and Escherichia coli were determined at high resolution. The folding patterns of the polypeptide chains proved to be identical to those of the wild-type enzymes, small conformational differences being restricted to parts of the active site. If aspartate or glutamate was added to the pyridoxal form of the mutant enzyme [lambda max 392 nm and 330 nm (weak); negative CD at 420 nm, positive CD at 370 nm and 330 nm], the external aldimine (lambda max = 430 nm; negative CD at 360 nm and 430 nm) transiently accumulated. Upon addition of 2-oxoglutarate to the pyridoxamine form (lambda max 330 nm, positive CD), a putative ketamine intermediate could be detected; however, with oxalacetate, an equilibrium between external aldimine and the pyridoxal form, which was strongly in favour of the former, was established within seconds. The transamination cycle with glutamate and oxalacetate proceeds only three orders of magnitude more slowly than the overall reaction of the wild-type enzyme. The specific activity of the mutant enzyme is 0.1 U/mg at 25 degrees C and constant from pH 6.0 to 8.5. Reconstitution of the mutant apoenzyme with [4'-3H]pyridoxamine 5'-phosphate resulted in rapid release of 3H with a first-order rate constant kappa' = 5 x 10(-4) s-1 similar to that of the wild-type enzyme. Apparently, in aspartate aminotransferase, histidine can to some extent substitute for the active-site lysine residue. The imidazole ring of H258, however, seems too distant from C alpha and C4' to act efficiently as proton donor/acceptor in the aldimine-ketamine tautomerization, suggesting that the prototropic shift might be mediated by an intervening water molecule. Transmination of the internal to the external aldimine apparently can be replaced by de novo formation of the latter, and by its hydrolysis in the reverse direction.     

A High-Frequency CMOS Current Summing Circuit

This work presents a signal summing circuit intended for application in a high-frequency linear equalizer. The circuit is capable of operating at frequencies reaching 10 GHz when implemented on a 0.18 CMOS process.     

NOx Reduction in the Exhaust of Mobile Heavy-Duty Diesel Engines by Urea-SCR

A DeNO _x demonstration system for a diesel engine used in construction machineries and mobile cranes was setup. In preliminary experiments various extruded and coated SCR catalysts were evaluated with and without oxidizing pre-catalyst. The data from stationary tests with two selected catalysts were used to establish various model-based control algorithms for the optimum dosage of urea in the ESC and ETC. A NO _x conversion of >93% at <10 ppm average ammonia slip could be achieved at a converter-to-swept volume ratio of <2.0.     

NO x reduction in the exhaust of mobile heavy-duty diesel engines by urea-SCR

Topics in Catalysis - A DeNO x demonstration system for a diesel engine used in construction machineries and mobile cranes was setup. In preliminary experiments various extruded and coated SCR...     

Caffeine release of radiation induced S and G2 phase arrest in V79 hamster cells: increase of histone messenger RNA levels and p34cdc2 activation

The serine/threonine protein kinase p34cdc2 activity in V79 hamster cells 4 h after treatment with 7-Gy X-rays is similar to that of unirradiated cells. Nevertheless, the irradiated cells are arrested in the S and G2 phases of the cell cycle. The mRNA concentrations of histones H1 and H4 are reduced by a factor of about 2 in irradiated cells compared to unirradiated cells, as opposed to the mRNAs of high-mobility group I(Y) and 17 proteins which appear unchanged. Both the p34cdc2 activity and the mRNA concentrations of the histones rise within 30 min after the release of the radiation induced cell cycle block by caffeine. During this time span the p34cdc2 activity increases about 4-fold and the histone mRNA levels recover approximately to those of an exponentially growing cell population. Regulatory pathways influenced in irradiated and in subsequently caffeine treated cells apparently interact with basic cell cycle control mechanisms.