The Escherichia coli RecA protein complements recombination defective phenotype of the Saccharomyces cerevisiae rad52 mutant cells

The Saccharomyces cerevisiae rad52 mutants are sensitive to many DNA damaging agents, mainly to those that induce DNA double-strand breaks (DSBs). In the yeast, DSBs are repaired primarily by homologous recombination (HR). Since almost all HR events are significantly reduced in the rad52 mutant cells, the Rad52 protein is believed to be a key component of HR in S. cerevisiae. Similarly to the S. cerevisiae Rad52 protein, RecA is the main HR protein in Escherichia coli. To address the question of whether the E. coli RecA protein can rescue HR defective phenotype of the rad52 mutants of S. cerevisiae, the recA gene was introduced into the wild-type and rad52 mutant cells. Cell survival and DSBs induction and repair were studied in the RecA-expressing wild-type and rad52 mutant cells after exposure to ionizing radiation (IR) and methyl methanesulphonate (MMS). Here, we show that expression of the E. coli RecA protein partially complemented sensitivity and fully complemented DSB repair defect of the rad52 mutant cells after exposure to IR and MMS. We suggest that in the absence of Rad52, when all endogenous HR mechanisms are knocked out in S. cerevisiae, the heterologous E. coli RecA protein itself presumably takes over the broken DNA.     

Epidemiology of end‐stage renal disease and hemodialysis treatment in Serbia at the turn of the millennium

The study presents the epidemiological features of patients treated with renal replacement therapy (RRT) in Serbia from 1997 to 2009 and compares the results of hemodialysis treatment in 1999 and 2009. Epidemiological data were obtained from the National Registry of RRT patients and data on hemodialysis treatment from special surveys conducted in 1999 and 2009. Within the period 1997–2009 the incidence of patients on RRT increased from 108 to 179 per million population (pmp), prevalence rose from 435 to 699 pmp, while mortality rate fell from 20.7% to 16.7%. The frequency of patients with glomerulonephritis decreased, while that of patients with diabetes and hypertensive nephropathy increased. In late 2009 there were 5208 patients receiving RRT in Serbia. Within the examined period new hemodialysis and reverse osmosis equipment were purchased, high‐flux dialyzers with synthetic membranes were increasingly used and the number of patients receiving hemodiafiltration increased to 17.6%. Kt/V greater than 1.2 was recorded in 16% of the patients in 1999 but 52% in 2009. Options for correction of anemia and mineral disorders have also improved. The percentage of patients with HbsAg (13.8% vs. 4.8%) as well as anti‐hepatitis C virus antibodies positive patients (23.2% vs. 12.7%) was significantly lower in 2009 than in 1999. Both the incidence and prevalence of RRT patients in Serbia are rising continuously, while the mortality rate is falling. More favorable conditions for dialysis treatment have brought about significant improvement in the results over the last 10 years.     

Terminological (di) Similarities between Information Management and Knowledge Management: a Term Co-Occurrence Analysis

The terminological composition of information management (IM) and knowledge management (KM) is described in order to evidence how much do these areas overlap within the library and information sciences (LIS) space. A term co-occurrence analysis is conducted as from documents extracted from Web of Science, covering the period 1980–2015. To examine the terminological (di) similarity two approaches are applied: ‘overlay visualization? and ‘topic detection?. The percentage of terminological similarity oscillates between 24% and 38% according to the ‘overlay visualization? and ‘topic detection? techniques. At the core of the terminological intersection between IM and KM lies the study around processes, technologies and information systems. However, they are semantically two separate areas that tend to address similar dimensions -such as the managerial, educational and medical, but emphasizing different approach. Moreover, IM refers to the library and information organizations, highlighting the focus on personal information management, human information behavior, health information management, and information resource management. Oppositely, KM highlights the business, management and accounting realms in which topics like knowledge sharing and social networks, knowledge management strategies, and knowledge management projects are prevailing. These results would help to (re) define curricular contents of LIS programs as well as research and practical activities conducted by academicians and managers. This is the most comprehensive quantitative study in examining the epistemic borders between IM and KM in the LIS research. The authors consider that the methodological procedures here employed might be suitable to understand the boundaries between similar fields.

     

Functionality of chitosan-halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass

This study was designed to investigate functionality of tetracycline-loaded chitosan-halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan. SEM analysis revealed compact, nonporous and rough surface of the nanocomposite films due to the presence of halloysite agglomerates and tetracycline crystals. Increasing chitosan molar mass led to higher values of elongation at break (from 21.65 ± 2.65 to 34.48 ± 2.34%), tensile strength (from 134.8 ± 13.21 to 246.36 ± 14.69 MPa), and elastic modulus (from 633.79 ± 128.37 to 716.55 ± 60.76 MPa) of the nanocomposite films. FT-IR, XRPD, and thermal analyses confirmed molar mass dependent chitosan-halloysite interactions and improved thermal stability of the nanocomposite films in comparison with chitosan films. The nanocomposite films released tetracycline in a sustained manner, with the slowest release achieved from the films consisting of low molar mass chitosan. Chitosan molar mass was confirmed to be a functionality-related characteristic of chitosan-halloysite nanocomposite films as potential sustained-release carriers for topical delivery of antibiotics. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020 , 137, 48406.     

Development of hydrophilic gels containing coenzyme Q10-loaded liposomes: characterization,stability and rheology measurements

Objective: The aim of this study was to develop, characterize and evaluate stability of a gel containing coenzyme Q₁₀ (Q₁₀)-loaded liposomes, and enhance the stability of Q₁₀ in the nanocarrier-containing gel compared to the conventional gel.

Methods: Q₁₀-loaded liposome dispersions prepared from unsaturated or saturated lecithin, were characterized for particle size, polydispersity index (PDI), zeta-potential, pH value, oxidation index, Q₁₀-content and morphology, and incorporated into carbomer gel. Liposome gels and liposome-free gel were analyzed for flow properties, pH values, Q₁₀-content, and liposomes size and PDI (liposome gels), 48?h after preparation and in predetermined time intervals during 6?months storage at different temperatures in order to predict their long term stability.

Results: Liposomes were of small particle size, homogeneous, negatively charged, and their incorporation into gel did not significantly change (p?>?.05) their particle size and PDI. All gels revealed non-Newtonian, shear-thinning plastic flow behavior during storage with no marked changes in rheological parameters. Storage of gels did not significantly influence the pH value (p?>?.05), while it significantly decreased Q₁₀-content (p?<?.05). Q₁₀ was significantly more (p?<?.05) stable in liposome gel containing unsaturated lecithin liposomes (G1) than in gel containing saturated lecithin liposomes (G2) and liposome-free gel (G3).

Conclusions: Q₁₀-loaded liposome gel G1 was the optimal formulation, since during storage at different temperatures, it did not show significant increase in liposome size and PDI, it provided significantly higher stability for Q₁₀ than other gels and its pH value was suitable for skin application. Due to limited Q₁₀-stability it should be stored at 4?°C.     

Dissolution of alumina ceramics in HCl aqueous solution

Dissolution of a cold isostatically pressed high purity alumina ceramics in aqueous HCl solutions was studied as a function of immersion time and acid concentration. From the amounts of Al³⁺, Mg²⁺, Ca²⁺, Na⁺, Si⁴⁺ and Fe³⁺ ions released in the corrosive solution, a degree of dissolution χ_i for each component was calculated according to the equation χ_i = A/B, where A and B are respectively the amount of the element released in the corrosive solution and the amount of the element in the untreated material. The determination of the amounts of ions released in the corrosive solutions was carried out by means of atomic absorption spectrometry (AAS). The corrosion of alumina ceramics in the HCl aqueous solution is determined by the solubility of alumina and the solubility of grain-boundary impurities. Very low dissolution values of Al³⁺, Mg²⁺, Ca²⁺, Na⁺, Si⁴⁺ and Fe³⁺ ions after the corrosion test of alumina ceramics showed a very good corrosion resistance in the HCl aqueous solution.     

Vehicle matching in smart camera networks using image projection profiles at multiple instances

Tracking vehicles using a network of cameras with non-overlapping views is a challenging problem of great importance in traffic surveillance. One of the main challenges is accurate vehicle matching across the cameras. Even if the cameras have similar views on vehicles, vehicle matching remains a difficult task due to changes of their appearance between observations, and inaccurate detections and occlusions, which often occur in real scenarios. To be executed on smart cameras the matching has also to be efficient in terms of needed data and computations. To address these challenges we present a low complexity method for vehicle matching robust against appearance changes and inaccuracies in vehicle detection. We efficiently represent vehicle appearances using signature vectors composed of Radon transform like projections of the vehicle images and compare them in a coarse-to-fine fashion using a simple combination of 1-D correlations. To deal with appearance changes we include multiple observations in each vehicle appearance model. These observations are automatically collected along the vehicle trajectory. The proposed signature vectors can be calculated in low-complexity smart cameras, by a simple scan-line algorithm of the camera software itself, and transmitted to the other smart cameras or to the central server. Extensive experiments based on real traffic surveillance videos recorded in a tunnel validate our approach.     

2,2′-Bipyridine-Modified Tamoxifen: A Versatile Vector for Molybdacarboranes

Investigations on the antitumor activity of metallacarboranes are sparse in the literature and limited to a handful of ruthena- and molybdacarboranes. In this study, the molybdacarborane fragment [3-(CO)₂-closo-3,1,2-MoC₂B₉H₁₁] was combined with a vector molecule, inspired by the well-known drug tamoxifen or 4,4′-dihydroxytamoxifen (TAM-diOH). The molybdacarborane derivative [3,3-{4-[1,1-bis(4-hydroxyphenyl)but-1-en-2-yl]-2,2′-bipyridine-κ²N,N′}-3-(CO)₂-closo-3,1,2-MoC₂B₉H₁₁] ( 10 ), as well as the ligand itself 4-[1,1-bis(4-hydroxyphenyl)but-1-en-2-yl]-2,2′-bipyridine ( 6 ) showed cytotoxic activities in the low micromolar range against breast adenocarcinoma (MDA-MB-231, MDA-MB-361 and MCF-7), human glioblastoma (LN-229) and human glioma (U-251) cell lines. In addition, compounds 6 and 10 were found to induce senescence and cytodestructive autophagy, lower ROS/RNS levels, but only the molybdacarborane 10 induced a strong increase of nitric oxide (NO) concentration in the MCF-7 cells.     

pH‐sensitive polyelectrolyte films derived from submicron chitosan/Eudragit® L 100‐55 complexes: Physicochemical characterization and in vitro drug release

The objectives of this study were to prepare films from submicron chitosan/Eudragit^® L100‐55 polyelectrolyte complexes (CH/EL PEC) and to assess the influence of CH molecular weight and CH/EL mass ratio on their structure and drug‐release properties. The films were obtained by a simple, environmentally friendly, casting/solvent evaporation method and the verapamil hydrochloride (VH) was used as model drug. Submicron size, narrow size distribution, and acceptable stability of CH/EL PECs were confirmed by DLS and laser Doppler microelectrophoresis. SEM analysis revealed nonporous inner structure and flat surface of the films. Interactions between comprising polymers and formation of CH/EL PEC were established by DSC and FT‐IR spectroscopy. In vitro swelling and drug release studies revealed the pH sensitivity of the films, with burst drug release in acidic conditions (pH 1.2) and sustained release in phosphate buffers pH 5.8, 6.8, and 7.4. The slowest VH release was achieved from the films prepared from equal amounts of EL and CH of higher molecular weight, confirming the significance of the CH/EL ratio and CH molecular weight on their ability to sustain drug release. The obtained results suggested that presented, simple, and eco‐friendly preparation procedure can be used to obtain pH‐sensitive CH/EL PEC films with a promising potential as drug carriers. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 42583.     

Corrosion behavior of alumina ceramics in aqueous HCl and H2SO4 solutions

The corrosion behavior of cold isostatically pressed (CIP) high purity alumina ceramics in aqueous HCl and H₂SO₄ solutions with various concentrations has been studied simultaneously at room temperature (25 °C). Corrosion tests were also performed with 0.65 mol/l HCl and 0.37 mol/l H₂SO₄ solutions at 40, 55 and 70 °C for 48 h. Chemical stability was monitored by determining the amount of Al³⁺, Mg²⁺, Ca²⁺, Na⁺ Si⁴⁺ and Fe³⁺ ions eluted in different concentrations of HCl and H₂SO₄ solutions by means of atomic absorption spectrometry (AAS). By increasing the concentration from 0.37 to 6.5 mol/l, it was notified that the corrosion susceptibility in HCl and H₂SO₄ solutions for the CIP alumina specimens at room temperature decreases.