Magnetically Guidable Proteinaceous Adhesive Microbots for Targeted Locoregional Therapeutics Delivery in the Highly Dynamic Environment of the Esophagus

The esophagus is a tubular-shaped muscular organ where swallowed fluids and muscular contractions constitute a highly dynamic environment. The turbulent, coordinated processes that occur through the oropharyngeal conduit can often compromise targeted administration of therapeutic drugs to a lesion, significantly reducing therapeutic efficacy. Here, magnetically guidable drug vehicles capable of strongly adhering to target sites using a bioengineered mussel adhesive protein (MAP) to achieve localized delivery of therapeutic drugs against the hydrodynamic physiological conditions are proposed. A suite of highly uniform microparticles embedded with iron oxide (IO) nanoparticles (MAP@IO MPs) is microfluidically fabricated using the genipin-mediated covalent cross-linking of bioengineered MAP. The MAP@IO MPs are successfully targeted to a specific region and prolongedly retained in the tubular-structured passageway. In particular, orally administered MAP@IO MPs are effectively captured in the esophagus in vivo in a magnetically guidable manner. Moreover, doxorubicin (DOX)-loaded MAP@IO MPs exhibit a sustainable DOX release profile, effective anticancer therapeutic activity, and excellent biocompatibility. Thus, the magnetically guidable locomotion and robust underwater adhesive properties of the proteinaceous soft microbots can provide an intelligent modular approach for targeted locoregional therapeutics delivery to a specific lesion site in dynamic fluid-associated tubular organs such as the esophagus.     

Isolation of antimicrobial agent from the marine algae Cystoseira hakodatensis

Cystoseira hakodatensis is an unutilised brown algae belonging to family Sargassaceae. A crude methanol extract from the algae showed inhibitory effects on the growths of Bacillus cereus and Bacillus licheniformis. To isolate the major antimicrobial agent, a sequential active‐guided isolation procedure was applied: liquid–liquid extraction, column chromatography and bio‐autography. A marked antimicrobial agent (active α) was isolated in hydrophobic fraction and was determined to phenolics without carbohydrates and proteins by phytochemical test. Regarding the antimicrobial potential, the isolated active α showed better inhibitory effects against B. cereus and B. licheniformis at 2 and 4 times of lower concentrations (62.5 and 31.3 μg mL^?1) in comparison with epigallocatechin gallate. These results showed that C. hakodatensis is a potential source of antimicrobial agent capable of preventing the growth of the two bacteria.     

Protocol misbehavior detection framework using machine learning classification in vehicular Ad Hoc networks

Wireless Networks - A novel approach is proposed to detect protocol misbehavior using state-of-the-art machine learning frameworks and entropy. Nodes in Vehicular Ad Hoc Networks (VANETs) use...     

Why does it pay to be selfish in a MANET?

Routing protocols for a mobile ad hoc network have assumed that all mobile nodes voluntarily participate in forwarding others' packets. This was a reasonable assumption because all MNs in a MANET belonged to a single authority. In the near future, however, a MANET may consist of MNs that belong to many different organizations since numerous civilian applications are expected to crop up. In this situation, some MNs may run independently and purposely decide not to forward packets so as to save their own energy. This could potentially lead to network partitioning and corresponding performance degradation. To minimize such situations in MANETs, many studies have explored the use of both the carrot and the stick approaches by having reputation-based, credit-payment, and game theory schemes. This article summarizes existing schemes, identifies their relative advantages, and projects future directions     

Effects of surface roughness of substrates on the c-axis preferred orientation of ZnO films deposited by r.f. magnetron sputtering

The c-axis preferred orientation of ZnO film is the most important factor for its successful application in piezoelectric devices. The effects of surface roughness of the substrate on the c-axis preferred orientation of ZnO thin films, deposited by radio frequency magnetron sputtering, were investigated. During sputtering, the oxygen content in the argon environment used was varied from 0 to 70% at a total sputtering pressure of 10 mTorr. Very smooth Si, smooth evaporated Au/Si, smooth evaporated-Al/Si, and rough sputtered-Al/Si were used as substrates. Their r.m.s. roughnesses, as measured by atomic force microscopy, were 1.27, 17.1, 21.1 and 65-118 Å, respectively. The crystalline structure and the angular spread of the (0 0* 2) plane normal to the ZnO films were determined using X-ray diffraction and X-ray rocking curves, respectively. The crystallinity and the preferred c-axis orientation of the ZnO films were strongly dependent on the surface roughness of the substrates rather than on the oxygen content of the working environment or on the chemical nature of the substrate.     

Performance analysis of OFDMA uplink systems with symbol timing misalignment

This letter presents our investigation for the effect of symbol timing errors in orthogonal frequency-division multiple access (OFDMA) uplink systems. We express the symbol timing errors between users as the symbol timing misalignments with respect to the desired user. Then, we derive an explicit expression of the signal-to-noise ratio (SNR) as a function of the maximum value of the symbol timing misalignments. Analyses and simulation results show that, to achieve an SNR of 20 dB, the maximum value of the symbol timing misalignments must be less than the cyclic prefix duration plus 6.25% of the useful symbol duration. Based on the resulting SNR degradation, we evaluate the SNR gain with guard subcarriers in order to mitigate the effect of the symbol timing misalignments.     

A three-dimensional FE analysis of large deformations for impact loadings using tetrahedral elements

 A three-dimensional dynamic program for the anaysis of large deformations in contact-penetration problems is developed using the finite element Lagrangian method with explicit time integration. By incorporating a tetrahedral element, which allows a single-point integration without a special hourglass control scheme, this program can be more effective to the present problem. The position code algorithm is used to search contact surface. Eroding surfaces are also considered. The defense node algorithm was slightly modified for the calculation of contact forces. A study of obliquity effects on metallic plate perforation and ricochet processes in thin plates impacted by a sphere was conducted. It is well simulated that on separation of two parts of the sphere, the portion still within the crater tends to perforate, while the portion in contact with the plate surface ricochets. This deformation pattern is observed in experiments, especially at high obliquities. A long rod that impacts an oblique steel plate at high impact velocity was also simulated in order to study the dynamics of the rod caused by the three dimensional asymmetric contact. The agreement between simulated and experimental results is quite good. Fracture phenomena occuring at high obliquity deserves further investigations. Received: 20 February 2002 / Accepted: 20 September 2002