Reflectometry by means of optical-coherence modulation

Novel reflectometry with millimetre or submillimetre spatial resolution is proposed to evaluate optical components or circuits. The optical coherence is modulated to have a periodic delta-function shape along the optical path, and the backscattering intensity is obtained directly. The experiment demonstrates a resolution of about 10 mm in air with a Fabry-Perot laser diode.<>     

Relationship between Expression of Onco-Related miRNAs and the Endoscopic Appearance of Colorectal Tumors

Accumulating data indicates that certain microRNAs (miRNAs or miRs) are differently expressed in samples of tumors and paired non-tumorous samples taken from the same patients with colorectal tumors. We examined the expression of onco-related miRNAs in 131 sporadic exophytic adenomas or early cancers and in 52 sporadic flat elevated adenomas or early cancers to clarify the relationship between the expression of the miRNAs and the endoscopic morphological appearance of the colorectal tumors. The expression levels of miR-143, -145, and -34a were significantly reduced in most of the exophytic tumors compared with those in the flat elevated ones. In type 2 cancers, the miRNA expression profile was very similar to that of the exophytic tumors. The expression levels of miR-7 and -21 were significantly up-regulated in some flat elevated adenomas compared with those in exophytic adenomas. In contrast, in most of the miR-143 and -145 down-regulated cases of the adenoma-carcinoma sequence and in some of the de novo types of carcinoma, the up-regulation of oncogenic miR-7 and/or -21 contributed to the triggering mechanism leading to the carcinogenetic process. These findings indicated that the expression of onco-related miRNA was associated with the morphological appearance of colorectal tumors.     

Dual voltage converter with improved power factor and enabling voltage control

In this paper, a novel version of the dual voltage converter is presented with improved voltage regulation and enabling a wide range of voltage control by using self-turn-off devices (power transistors) instead of diodes. Further, this system operates with unity displacement factor. We have obtained the analytical expressions of this circuit. The results are, however, quite complex. We could calculate values of the voltage and current waveforms by using a computer. The calculated values are essentially in agreement with the experimental values. Experimentally, an ideal load characteristic with null voltage regulation was obtained by the conduction angle control for power transistors. Further, a wide range of voltage from 220 V to 39 V was obtained. A disadvantage for this system is the requirement for snubber circuits with a larger value of capacitor in order to suppress the surge voltages at turn-off periods.     

Detection of six single-nucleotide polymorphisms associated with rheumatoid arthritis by a loop-mediated isothermal amplification method and an electrochemical DNA chip

An electrochemical DNA chip using an electrochemically active intercalator and DNA probe immobilized on a gold electrode has been developed for genetic analysis. In this study, the six polymorphisms associated with rheumatoid arthritis (RA), N-acetyltransferase2 (NAT2) gene polymorphisms T341C, G590A, and G857A, methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms C677T and A1298C, and serum amyloid A1 (SAA1) gene promoter polymorphism C-13T were simultaneously detected by the electrochemical DNA chip and the loop-mediated isothermal amplification (LAMP) method, which is a novel technique for DNA amplification. Human genomic DNAs were extracted from blood, and the targets containing the six polymorphisms were amplified by the LAMP method. A sample containing the six LAMP products was reacted with the electrochemical DNA chip using a DNA detection system that controls hybridization reaction, washing, electrochemical detection, and data analysis automatically. A total of 31 samples were genotyped by this method, and the results were completely consistent with those determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis or the PCR direct sequence analysis. The time required for this method was only 2 h, and operations were very simple. Therefore, this method is expected to contribute to personalized medicine based on genotype.     

A mathematical model for computer simulation of the direct continuous esterification process between terephthalic acid and ethylene glycol. Part II: Reaction rate constants

It has been confirmed that the reaction model proposed previously, which can express simultaneously the oligomer properties and the distillate properties under low esterification pressure, is applicable to a continuous direct esterification process in a practical plant. The experimental data of the first esterification reactor (RA-1) was obtained under low reaction pressures (atomospheric or 1 kg/cm²G) with the pilot plant throughput based on poly(ethylene terephthalate) (PET) polymer production of about 50 kg/h. The Arrhenius' parameters, frequency factor, and apparent activation energy, were determined fitting the experimental data of the pilot plant by using the Simplex method as an optimization technique. The activation energy of diethylene glycol (DEG) formation, E₇, is about twice as much as those of the esterifications, E₁, E₂, E₃, and E₄. The activation energies are E₁, = 19640 cal/mol, E₂ = 18140, E₃ = 22310, E₄ = 18380, E₅ = 2810, E₆ = 14960, and E₇ = 42520 cal/mol. Good agreement was obtained between experimental data and calculated predictions for several oligomer and distillate properties. The vapor-liquid equilibrium can be expressed by Raoult's law with little problem of practical use.     

Effects of a barrier on creeping discharge characteristics in SF6 and N2 gases under pulse voltages

This paper describes the effect of a barrier on creeping discharge characteristics in SF₆, N₂, and their mixtures. The barrier height effect on the discharge voltage was investigated under a positive pulse voltage. The discharge voltage increased with increasing barrier height in SF₆ gas. On the other hand, the discharge voltage hardly changed with the barrier height in N₂ gas. In order to clarify the mechanism of the initial creeping corona, it was observed by optical techniques, including an ultra-high-speed electronic imaging system (IMACON 468). The creeping corona path revealed differences in images at various barrier heights. The accumulated charge induced on the barrier by the initial corona in SF₆ gas was able to suppress the development of the later creeping corona, in contrast with N₂ gas. © 1998 Scripta Technica, Electr Eng Jpn, 125(4): 1–8, 1998     

Molecular basis for the interaction between human IgM and staphylococcal protein A

To examine the relationship between VH gene usage and reactivity of immunoglobulins, we cloned B cells from peripheral blood from adults and from human neonatal cord blood by EBV transformation. Nearly one-third of the B cell clones from both sources produced IgM reactive with staphylococcal protein A (SPA). None of such IgM reacted with other antigens, except for the crude extract of Staphylococcus aureus. All of 22 B cell clones producing IgM reactive with SPA expressed VH3 genes, while none of the control 15 clones secreting IgM nonreactive with SPA expressed VH3. The IgM proteins reactive with SPA could be clearly divided into two subjects based on the differential binding avidity to solid-phase SPA. Both kappa and lambda light chains were used in each subset of SPA-reactive IgM. Sequence analysis of the PCR products from seven VH3-IgM clones revealed that the VH3 genes were used in nearly germline configuration. The D and J gene usage was diverse. Comparison of amino acid sequences between antibodies with high and low avidity to SPA suggests that the differential avidity is related to amino acid sequence differences in the complementarity determining region 2 and framework region 3. The high frequencies of B cells committed to the production of SPA-reactive IgM in normal blood and the restricted use of VH3 heavy chain genes in nearly germline configuration in these cells support the notion that SPA behaves like a superantigen toward human B cells.     

A TEA‐CO2 laser oscillation by an inductive energy storage pulsed‐power generator using a wire‐fuse opening switch

We have carried out experiments on TEA‐CO₂ laser oscillation using the inductive energy storage pulsed‐power generator, which has a copper wire fuse as an opening switch. Maximum laser output energy of about 1 J/pulse was obtained in the case of a fuse length of 5 cm and energy storage inductance of 8 μH. The laser output energy depends on the energy storage inductance and the parameters of the fuse. In this paper, the dependencies of laser output energy on inductance and fuse length, and a comparison between the inductive and capacitive system were described. Furthermore the laser efficiency was discussed by calculating the electron energy distribution of laser main discharge region. © 2000 Scripta Technica, Electr Eng Jpn, 132(1): 15–21, 2000