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排序方式: 共有92条查询结果,搜索用时 31 毫秒
1.
Keitaro Enami 《Engineering Fracture Mechanics》2005,72(7):1089-1105
It has been recognized that ductility of prestrained steel is inferior to that without prestrain, and the critical equivalent plastic strain of ductile fracture initiation is inversely related to stress triaxiality. In this paper, the effects of compressive and tensile prestrain on ductile fracture initiation in steels are investigated quantitatively by adopting the relationship between stress triaxiality and critical equivalent plastic strain. It is found that compressive prestrain leads to cleavage cracking and reduces ductility. In the case of the TMCP steel, compressive prestrain up to 30% does not decrease the ductility, accompanied by no evidence of cleavage cracks. However, in the case of SM490B steel, 30% compressive prestrain leads to cleavage cracking and reduces ductility significantly. 相似文献
2.
Dr. Masanobu Nagano Takumi Oguro Ryo Sawada Dr. Toru Yoshitomi Prof. Keitaro Yoshimoto 《Chembiochem : a European journal of chemical biology》2021,22(23):3341-3347
Dysregulation of tumor necrosis factor-α (TNFα), a pro-inflammatory cytokine, causes several diseases, making it an important therapeutic target. Here, we identified a novel DNA aptamer against human TNFα using in vitro selection, which included a high exclusion pressure process against non-binding and weak binders through microbead-assisted capillary electrophoresis (MACE) in only three rounds. Among the 15 most enriched aptamers, Apt14 exhibited the highest inhibitory activity for the interaction between TNFα and its cognate receptor in mouse L929 cells. For further improving the bioactivity of the aptamer, dimerization programed by hybridization was evaluated, resulting in the Apt14 dimer exhibited a twofold higher binding affinity and stronger inhibition compared to the monomer counterpart. Rapid identification of bioactive aptamers using MACE in combination with facile dimerization by hybridization accelerates the discovery of novel bioactive aptamers, paving the way toward replacing current monoclonal antibody therapy with the less expensive and non-immunogenic aptamer therapy. 相似文献
3.
Masatake Ohnishi Atsuko Higuchi Setsuko Todoriki Keitaro Hiromi Wmikio Ohgushi Akiyoshi Wada 《Starch - St?rke》1990,42(7):273-276
Using the pH-jump (neutral to above 11) for Tyr-OH ionization as a probe, two glucoamylases from Rhizopus niveus and Rhizopus delemar were found to be caused the change in conformation, which is in a kinetically single step. The pH titration at pHs below 12 was carried out on a multidimensional correlation spectrophotometer. Correlations among the spectrophotometric properties: fluorescence, absorption and circular dichroism are almost identical for the two enzymes. These results suggest that conformational characteristics of the niveus enzyme is almost the same as that of the delemar one. 相似文献
4.
Based on the subsite structure of an α-amylase from Bacillus subtilis, production of glucose from a substrate maltohexaose in the amylase-catalyzed reaction was theoretically predicted as a function of time. The theoretical time course was found consistent with the experimental results, which were obtained by the determination of a product glucose with a commercial assay-kit. Hence, glucose, product from a substrate maltohexaose, will be a useful probe for the amylase-catalyzed reaction. 相似文献
5.
Using maltohexaose G6 as a substrate, a convenient analytical method for amylase-catalyzed hydrolytic reaction was developed by application of an enzymatic assay-kit (glucose oxidase-peroxidase) to measurement of glucose, produced on the reaction: G6→G5 + G. The initial velocity was confirmed to be proportional to the initial concentration of amylase, examined, ∼ 100 μm. The new method was found in a good correlation with the Nelson and Somogyi method. Rate assay was also examined: The initial velocity is proportional to the enzyme concentration, ∼ 30 μm. Hence, this enzymatic assay method is recognized to be convenient and useful for the measurement of α-amylase. 相似文献
6.
Masayuki Ohta Keitaro Kondo Masayuki Matsunaka Hiroyuki Miyamaru Isao Murata Toshiyuki Iida Kentaro Ochiai Chikara Konno 《Fusion Engineering and Design》2009,84(7-11):1446-1449
Integral benchmark experiments with DT neutrons are not always sufficient for nuclear data benchmarking in the MeV region, below 10 MeV. A neutron spectrum shifter, which will be placed between a sample and a DT neutron source, is effective to moderate DT neutrons incident to the sample. In order to estimate effects of the spectrum shifter, the ratio of the contribution of 14 MeV neutrons in the leakage neutron and gamma-ray spectra was calculated with MCNP-4C for an experimental configuration at FNS of JAEA, Japan. The calculations were carried out for a Li2TiO3 sample with a Be, D2O, or 7LiD spectrum shifter. It was found out that the Be shifter was superior to others and the Be shifter was effective to decrease the contribution of 14 MeV neutrons especially for secondary gamma-ray spectrum measurements. 相似文献
7.
This paper discusses robust seeking control of hard disk drives by the use of a variable structure multi-rate estimator (VSME). Compensation of pivot nonlinearity is one concern in the design of the seeking control, systems. Pivot nonlinearity is subject to uncertainties, and is difficult to deal with by a linear controller. The VSME estimates a class of uncertain disturbances and nonlinearities and yields compensation current through the linear feedback gain, which reduces the residual vibration and error during settling. Several experiments were carried out on several drives including a 3.5 in. 7,200 rpm drive. These experiments confirmed that the off-track probability after settling, which is defined by the number of seek failures over all trials, is much reduced to less than 1% when the drive is operated under a special temperature condition that increases pivot nonlinearity while the corresponding performance of a conventional linear controller is 12%. The seek failure is defined as the case when the position cannot be maintained within ±2.5% of a track pitch for a certain duration after track seeking mode. 相似文献
8.
Eitake Ibaragi Akira Hyogo Keitaro Sekine 《Analog Integrated Circuits and Signal Processing》1999,20(2):119-128
This paper proposes a novel low power dissipation technique for a low voltage OTA. A conventional low power OTA with a class AB input stage is not suitable for a low voltage operation (±1.5 V supply voltages), because it uses composite transistors (referred to CMOS pair) which has a large threshold voltage. On the other hand, the tail-current type OTA needs a large tail-current value to obtain a sufficient input range at the expense of power dissipation. Therefore, the conventional tail-current type OTA has a trade-off between the input range and the power dissipation to the tail-current value. The trade-off can be eliminated by the proposed technique. The technique exploits negative feedback control including a current amplifier and a minimum current selecting circuit. The proposed technique was used on Wang's OTA to create another OTA, named Low Power Wang's OTA. Also, SPICE simulations are used to verify the efficiency of Low Power Wang's OTA. Although the static power of Low Power Wang's OTA is 122 W, it has a sufficient input range, whereas conventional Wang's OTA needs 703 W to obtain a sufficient input range. However, we can say that as the input signal gets larger, the power of Low Power Wang's OTA becomes larger. 相似文献
9.
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