Synchronous Full-Scan for Asynchronous Handshake Circuits

Handshake circuits form a special class of asynchronous circuits that has enabled the industrial exploitation of the asynchronous potential such as low power, low electromagnetic emission, and increased cryptographic security. In this paper we present a test solution for handshake circuits that brings synchronous test-quality to asynchronous circuits. We add a synchronous mode of operation to handshake circuits that allows full controllability and observability during test. This technique is demonstrated on some industrial examples and gives over 99% stuck-at fault coverage, using test-pattern generators developed for synchronous circuits. The paper describes how such a full-scan mode can be achieved, including an approach to minimize the number of dummy latches in case latches are used in the data path of the handshake circuit.     

Testability-analysis driven test-generation of analogue cores

A new definition of the testability transfer factor for circuit components that provides better sensitivity with respect to parametric deviations is presented. New equations for the testability measures in a mixed-signal core are given. Testability analysis is used for test-pattern generation and for consideration of inserting wrapper cells. The simulation results show the effectiveness of the approach.     

ADC Multi-Site Test Based on a Pre-test with Digital Input Stimulus

This paper describes two novel algorithms based on the time-modulo reconstruction method intended for detection of the parametric faults in analogue-to-digital converters (ADC). In both algorithms, a pulse signal, in its slightly adapted form to allow sufficient time for converter settling, is taken as the test stimulus relieving the burden placed on the accuracy requirement of the excitation source. Instead of calculating the accurate conventional dynamic and static parameters, a signature result is obtained through the analysis of the output data in the time domain. The basic concept of the algorithms is the evaluation on the performance of ADCs by the comparison of the similarity of the output waveforms. The multi-site test is expensive for traditional specification-based tests of ADCs, as high quality analogue data generators are required. Based on these two algorithms, this paper proposes a solution for this problem. The objective of the test scheme is not to completely replace traditional specification-based tests, but to provide a reliable method for early identification of excessive parameter variations in production test that allows quickly discarding of most of the faulty circuits before performing a conventional test. The efficiency of the methods is validated on an industrial 12-bit pipelined ADC both in simulations and in measurements.     

Bartonella serology for patients with intraocular inflammatory disease

AIMS: Five cases of primary gastrointestinal (GI) lymphoma (three in the stomach, one in the ileum (IPSID) and one in the colon) associated with localized AL amyloidosis were studied to identify morphological or immunohistochemical features which could explain the amyloid deposition. METHODS AND RESULTS: All the cases were low-grade marginal zone B-cell lymphomas; one case of gastric lymphoma and the IPSID also had a high-grade component. The lymphomas had a monoclonal plasma cell population, with different light and heavy-chain type expression in the five cases. Plasma cell differentiation was closely associated with the amyloid deposits. The latter were an incidental microscopic finding in one case, but produced tumoral masses in the other. CONCLUSIONS: The presence of amyloid in primary GI lymphoma is rare, but can have diagnostic value. In the present study, neither particular features of the lymphomatous proliferation nor specific agents are identified. Therefore, the factors predisposing to amyloid deposition require elucidation.     

Scan Test Strategy for Asynchronous-Synchronous Interfaces

In the coming years, the well-known synchronous design style will not be able to keep pace with the increase speed and capabilities of integration of advanced processes. New design paradigms, like core reuse of the already designed synchronous modules and asynchronous designs, are considered in order to cope with the ever increasing complexity. The future SoCs will contain multiple synchronous and asynchronous cores. Asynchronous design will become more and more common among digital designers, while synchronous-asynchronous interactions will emerge as a key issue in the future SoC designs.This paper will present test strategies for 2-phase asynchronous-synchronous interfaces and vice versa. It will be shown how test vectors can be automatically generated using commercially available ATPG tools. The generated ATPG vectors will be able to test all stuck-at-faults within the asynchronous-synchronous interfaces.     

Induction of cell proliferation in quiescent NIH 3T3 cells by oncogenic c-Raf-1

The c-Raf-1 kinase is activated by different mitogenic stimuli and has been shown to be an important mediator of growth factor responses. Fusion of the catalytic domain of the c-Raf-1 kinase with the hormone binding domain of the estrogen receptor (deltaRaf-ER) provides a hormone-regulated form of oncogenic activated c-Raf-1. We have established NIH 3T3 cells stably expressing a c-Raf-1 deletion mutant-estrogen receptor fusion protein (c-Raf-1-BxB-ER) (N-BxB-ER cells). The transformed morphology of these cells is dependent on the presence of the estrogen antagonist 4-hydroxytamoxifen. Addition of 4-hydroxytamoxifen to N-BxB-ER cells arrested by density or serum starvation causes reentry of these cells into cell proliferation. Increases in the cell number are obvious by 24 h after activation of the oncogenic c-Raf-1 protein in confluent cells. The onset of proliferation in serum-starved cells is further delayed and takes about 48 h. In both cases, the proliferative response of the oncogenic c-Raf-1-induced cell proliferation is weaker than the one mediated by serum and does not lead to exponential growth. This is reflected in a markedly lower expression of the late-S- and G2/M-phase-specific cyclin B protein and a slightly lower expression of the cyclin A protein being induced at the G1/S transition. Oncogenic activation of c-Raf-1 induces the expression of the heparin binding epidermal growth factor. The Jnk1 kinase is putatively activated by the action of the autocrine growth factor. The kinetics of Jnk1 kinase activity is delayed and occurs by a time when we also detect DNA synthesis and the expression of the S-phase-specific cyclin A protein. This finding indicates that oncogenic activation of the c-Raf-1 protein can trigger the entry into the cell cycle without the action of the autocrine growth factor loop. The activation of the c-Raf-1-BxB-ER protein leads to an accumulation of high levels of cyclin D1 protein and a repression of the p27Kip1 cyclin-dependent kinase inhibitor under all culture conditions tested.     

Fast fault simulation for nonlinear analog circuits

A new method of transient fault simulation uses dc bias grouping of faulty circuits and decreases the number of Newton-Raphson iterations needed to reach a solution. An experimental tool implementing this method achieves a speedup of 20% to 30% on a flat netlist.     

Design and analysis of planar monopole antennas using a genetic algorithm approach

The application of genetic algorithm (GA) optimization to the design and analysis of planar monopole antennas is presented. GA is first used to optimize the impedance matching bandwidth of two particular planar element shapes, the bow-tie (BT) and reverse bow-tie (RBT). The results of this study indicate that the RBT can achieve a significantly wider bandwidth with a much smaller size than the traditional BT. In a follow-on study, GA is used to generate arbitrarily shaped planar monopole designs, which exhibit improved broadband performance and/or reduced size compared with the RBT. The designs generated by the GA demonstrate a better tradeoff between matching bandwidth and electrical size compared with planar monopole designs previously characterized in the literature. Analysis of results from simulation and measurement are presented, which provide insight into the operation of these antennas as well as the key parameters that lead to improved performance. Finally, a performance bound is generated to relate the bandwidth limitation of planar monopoles to size.     

Microfluidics-Based Force Spectroscopy Enables High-Throughput Force Experiments with Sub-Nanometer Resolution and Sub-Piconewton Sensitivity

Several techniques have been established to quantify the mechanicals of single molecules. However, most of them show only limited capabilities of parallelizing the measurement by performing many individual measurements simultaneously. Herein, a microfluidics-based single-molecule force spectroscopy method, which achieves sub-nanometer spatial resolution and sub-piconewton sensitivity and is capable of simultaneously quantifying hundreds of single-molecule targets in parallel, is presented. It relies on a combination of total internal reflection microscopy and microfluidics, in which monodisperse fluorescent beads are immobilized on the bottom of a microfluidic channel by macromolecular linkers. Application of a flow generates a well-defined shear force acting on the beads, whereas the nanomechanical linker response is quantified based on the force-induced displacement of individual beads. To handle the high amount of data generated, a cluster analysis which is capable of a semi-automatic identification of measurement artifacts and molecular populations is implemented. The method is validated by probing the mechanical response polyethylene glycol linkers and binding strength of biotin–NeutrAvidin complexes. Two energy barriers (at 3 and 5.7 Å, respectively) in the biotin–NeutrAvidin interaction are resolved and the unfolding behavior of talin's rod domain R3 in the force range between 1 to ≈10 pN is probed.