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1.
Elena V. Mitroshina Maria M. Loginova Roman S. Yarkov Mark D. Urazov Maria O. Novozhilova Mikhail I. Krivonosov Mikhail V. Ivanchenko Maria V. Vedunova 《International journal of molecular sciences》2022,23(2)
Ischemic brain injury is a widespread pathological condition, the main components of which are a deficiency of oxygen and energy substrates. In recent years, a number of new forms of cell death, including necroptosis, have been described. In necroptosis, a cascade of interactions between the kinases RIPK1 and RIPK3 and the MLKL protein leads to the formation of a specialized death complex called the necrosome, which triggers MLKL-mediated destruction of the cell membrane and necroptotic cell death. Necroptosis probably plays an important role in the development of ischemia/reperfusion injury and can be considered as a potential target for finding methods to correct the disruption of neural networks in ischemic damage. In the present study, we demonstrated that blockade of RIPK1 kinase by Necrostatin-1 preserved the viability of cells in primary hippocampal cultures in an in vitro model of glucose deprivation. The effect of RIPK1 blockade on the bioelectrical and metabolic calcium activity of neuron-glial networks in vitro using calcium imaging and multi-electrode arrays was assessed for the first time. RIPK1 blockade was shown to partially preserve both calcium and bioelectric activity of neuron-glial networks under ischemic factors. However, it should be noted that RIPK1 blockade does not preserve the network parameters of the collective calcium dynamics of neuron-glial networks, despite the maintenance of network bioelectrical activity (the number of bursts and the number of spikes in the bursts). To confirm the data obtained in vitro, we studied the effect of RIPK1 blockade on the resistance of small laboratory animals to in vivo modeling of hypoxia and cerebral ischemia. The use of Necrostatin-1 increases the survival rate of C57BL mice in modeling both acute hypobaric hypoxia and ischemic brain damage. 相似文献
2.
Self-modulation of the optical absorption spectrum is observed during the picosecond photogeneration of charge carriers and intense superluminescence in GaAs. As the picosecond delay τ of the probing pulse with respect to the pump pulse is varied in the region of τ < 0, the local points of the absorption intensification (juts) shift along the spectrum (the modulation resembles a running wave). As the value of τ is varied in the vicinity of τ = 0, the juts in the spectrum arise and disappear at approximately fixed photon energies (the modulation resembles a standing wave). At certain photon energies, the dependence of the rate of variation in the absorption coefficient dα/dτ on τ is found to be modulated by pulsations, similarly to the previously observed modulation of the picosecond stimulated emission from GaAs. Presumably, the spectrum self-modulation represents (and, thus, reveals) the modulation of the electron distribution in the conduction band. This modulation is caused by the fact that the evolution of the electron-population depletion at the bottom of the conduction band during superluminescence reflects (due to the electron-phonon interaction) on the population of the upper energy levels in the band. 相似文献
3.
S. V. Nizhankovskiy A. Ya. Dan’ko E. V. Krivonosov V. M. Puzikov 《Inorganic Materials》2010,46(1):35-37
We report the growth of large (110 × 200 × 50 mm) titanium-doped sapphire crystals by horizontal directional solidification
in a protective atmosphere and their lasing characteristics. The crystals are shown to be suitable for the fabrication of
lasing elements with a figure of merit above 150 and wavefront distortion within λ/6. 相似文献
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Under picosecond photogeneration of charge carriers in GaAs, accompanied by intense stimulated emission of the semiconductor itself, ultrafast self-modulation of its light-absorption spectrum takes place, which consists in the appearance of regions of local absorption enhancement (bumps) in the spectrum. The ultrafast self-modulation is found to exhibit a cyclic behavior; i.e., the pattern of the self-modulation of the spectrum (the number and spectral position of the bumps) is repeated after a certain time T c falling in the picosecond range. The cycle period T c varies over the time span of the pump pulse and depends on the pulse energy, which means that T c is a function of the pump intensity. Assuming that self-modulation of the absorption reflects self-modulation of the charge-carrier energy distribution in GaAs under pumping, experimental results can be formulated as follows: in the process of the ultrafast self-modulation, deviations of the occupancies of different energy levels from the Fermi distribution evolve with time in a mutually related way; the distribution of the occupancy depletion in the conduction band repeats cyclically in time; and the cycle period decreases as the intensity of the pump increases. 相似文献
8.
Semiconductors - During the powerful picosecond optical pumping of a thin (~1 μm) GaAs layer, a stimulated intense (up to 1 GW/cm2) picosecond emission arises. It is found that the... 相似文献
9.
Semiconductors - During the powerful picosecond optical pumping, intense stimulated picosecond emission arises in a thin GaAs layer. It is found that, first, the maximum emission intensity... 相似文献
10.
Pumping a thin layer of GaAs with a high-power picosecond optical pulse leads to nonstationary edge emission. Experimental
data are obtained for the way the time-integrated power spectrum of this emission varies with beam diameter and energy of
the optical pump pulse. These data are sufficient to confirm the stimulated nature of the emission, whose duration is in the
picosecond time range.
Fiz. Tekh. Poluprovodn. 32, 537–541 (May 1998) 相似文献