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The regulation of programmed cell death in the developing nervous system involves target-derived survival factors, afferent synaptic activity, and hormone- and cytokine-dependent signaling. Cultured immature cerebellar granule neurons die by apoptosis within several days in vitro unless maintained in depolarizing (high) concentrations of potassium (25 mM K+). Here we report that transforming growth factors (TGF)-beta1, -beta2, and -beta3 accelerate apoptosis of these neurons when maintained in physiological (low) K+ medium (5mM K+) as assessed by measures of viability, quantitative DNA fragmentation, and nuclear morphology. TGF-beta-induced apoptosis of these neurons is not blocked by CNTF and LIF, cytokines that enhance neuronal survival when applied alone, or by IGF-I, which prevents apoptosis upon potassium withdrawal. In contrast, neurons that differentiate in high K+ medium for several days in vitro acquire resistance to TGF-beta-mediated cell death. Granule neurons maintained in either low or high K+ medium produce latent, but not bioactive, TGF-beta1 and -beta2. Because neutralizing TGF-beta antibodies fail to augment survival of low K+ neurons, the cerebellar neurons are apparently unable to activate latent TGF-beta. Thus, apoptosis of low K+ neurons is not attributable to endogenous production of TGF-beta. Taken together, our data suggest that TGF-beta may limit the expansion of postmitotic neuronal precursor populations by promoting their apoptosis but may support survival of those neurons that have maturated, differentiated, and established supportive synaptic connectivity. 相似文献
3.
Luca Scardovi Marco Baglietto Thomas Parisini 《Neural Networks, IEEE Transactions on》2007,18(4):1172-1184
In this paper, we consider the problem of actively providing an estimate of the state of a stochastic dynamic system over a (possibly long) finite time horizon. The active estimation problem (AEP) is formulated as a stochastic optimal control one, in which the minimization of a suitable uncertainty measure is carried out. Toward this end, the use of the Renyi entropy as an information measure is proposed and motivated. A neural control scheme, based on the application of the extended Ritz method (ERIM) and on the use of a Gaussian sum filter (GSF), is then presented. Simulation results show the effectiveness of the proposed approach. 相似文献
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In this paper, the authors propose two modeling procedures for wind speed simulation. These procedures could be implemented on the structure of a wind turbine simulator during studies concerning stand-alone or hybrid wind systems. The evolution of a horizontal wind speed has been synthesized taking into account two components. The medium- and long-term component is described by a power spectrum associated to a specific site. The turbulence component is assumed to be dependent on the medium- and long-term wind speed evolution. It is considered as a nonstationary process. Two simulation methods for this component, using rational and nonrational filters are proposed. In both procedures, the turbulence model is defined by two parameters, which are either obtained experimentally, or adopted a priori, according to information from the considered site. Numerical results and implementation aspects are also discussed. 相似文献
5.
Condia Josie E. Rodriguez Narducci Pierpaolo Sonza Reorda Matteo Sterpone Luca 《The Journal of supercomputing》2021,77(10):11625-11642
The Journal of Supercomputing - General-purpose graphics processing units (GPGPUs) are extensively used in high-performance computing. However, it is well known that these devices’... 相似文献
6.
Di Luca GA Mufarrege MM Sánchez GC Hadad HR Maine MA 《Water science and technology》2011,63(10):2374-2380
The aim of this work was to study the accumulation and fractionation of P in the inlet and outlet sediment of a constructed wetland for the wastewater treatment of a metallurgic plant in Argentina. It was important to predict whether P could be released into the water again by changing environmental conditions or retained over time. P-fractionation was performed using a sequential extraction method. Sediment cores were sliced at depths of: 0-3; 3-7 and 7-10 cm. Sediment showed high pH values and anoxic conditions. In the inlet area, P was principally bound to the carbonate fraction, whereas in the outlet area, it was mainly bound in the residual fraction. This behavior was justified by the effluent composition, which is rich in Ca2+ and Fe3+ and presents high values of pH and conductivity. These conditions favor CaCO3 and Fe(OOH)n precipitation and the subsequent sorption of P to their surface. The sediment active layer involved in the exchange reactions was the superficial one (0-3 cm). The wetland is highly efficient in P retention. P was retained by sediment in fractions that will not release it to the water while chemical and environmental conditions of the system are maintained. 相似文献
7.
Luca Aceto 《Formal Aspects of Computing》1994,6(2):201-222
This paper proposes alternative, effective characterizations for nets of automata of the location equivalence and preorder presented by Boudol et al. in the companion paper [BCHK]. Contrary to the technical development in the above given reference, where locations are dynamically associated to the subparts of a process in the operational semantics, the equivalence and preorder we propose are based on a static association of locations to the parallel components of a net. Following this static approach, it is possible to give these distributed nets a standard operational semantics which associates with each net a finite labelled transition system. Using this operational semantics for distributed nets, we introduce effective notions of equivalence and preorder which are shown to coincide with those proposed in [BCHK]. 相似文献
8.
Nordahl R Turchet L Serafin S 《IEEE transactions on visualization and computer graphics》2011,17(9):1234-1244
We propose a system that affords real-time sound synthesis of footsteps on different materials. The system is based on microphones, which detect real footstep sounds from subjects, from which the ground reaction force (GRF) is estimated. Such GRF is used to control a sound synthesis engine based on physical models. Two experiments were conducted. In the first experiment, the ability of subjects to recognize the surface they were exposed to was assessed. In the second experiment, the sound synthesis engine was enhanced with environmental sounds. Results show that, in some conditions, adding a soundscape significantly improves the recognition of the simulated environment. 相似文献
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Eric Pedrol Javier Martínez Magdalena Aguiló Manuel Garcia-Algar Moritz Nazarenus Luca Guerrini Eduardo Garcia-Rico Francesc Díaz Jaume Massons 《Microfluidics and nanofluidics》2017,21(12):181
This paper presents an optofluidic device for cell discrimination with two independent interrogation regions. Pumping light is coupled to the device, and cell fluorescence is extracted from the two interrogation zones by using optical fibers embedded in the optofluidic chip. To test the reliability of this device, AU-565 cells—expressing EpCAM and HER2 receptors—and RAMOS cells were mixed in a controlled manner, confined inside a hydrodynamic focused flow in the microfluidic chip and detected individually so that they could be discriminated as positive (signal reception from fluorescently labeled antibodies from the AU-565 cells) or negative events (RAMOS cells). A correlation analysis of the two signals reduces the influence of noise on the overall data. 相似文献