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1.
Dr. Yibin Zhang Dr. Shuai Xia Shulin Wan Tessa E. Steenwinkel Tara Vohs Prof. Rudy L. Luck Prof. Thomas Werner Prof. Haiying Liu 《Chembiochem : a European journal of chemical biology》2021,22(13):2282-2291
Abnormal levels of glutathione, a cellular antioxidant, can lead to a variety of diseases. We have constructed a near-infrared ratiometric fluorescent probe to detect glutathione concentrations in biological samples. The probe consists of a coumarin donor, which is connected through a disulfide-tethered linker to a rhodamine acceptor. Under the excitation of the coumarin donor at 405 nm, the probe shows weak visible fluorescence of the coumarin donor at 470 nm and strong near-infrared fluorescence of the rhodamine acceptor at 652 nm due to efficient Forster resonance energy transfer (FRET) from the donor to the acceptor. Glutathione breaks the disulfide bond through reduction, which results in a dramatic increase in coumarin fluorescence and a corresponding decrease in rhodamine fluorescence. The probe possesses excellent cell permeability, biocompatibility, and good ratiometric fluorescence responses to glutathione and cysteine with a self-calibration capability. The probe was utilized to ratiometrically visualize glutathione concentration alterations in HeLa cells and Drosophila melanogaster larvae. 相似文献
2.
This study examined whether objects are attended in serial or in parallel during a demanding visual search task. A component of the event-related potential waveform, the N2pc wave, was used as a continuous measure of the allocation of attention to possible targets in the search arrays. Experiment 1 demonstrated that the relative allocation of attention shifts rapidly, favoring one item and then another. In Experiment 2, a paradigm was used that made it possible to track the absolute allocation of attention to individual items. This experiment showed that attention was allocated to one object for 100-150 ms before attention began to be allocated to the next object. These findings support models of attention that posit serial processing in demanding visual search tasks. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
3.
The man-machine dialog is very important for the design process. In this paper a program system, based on the minicomputer KRS 4201 (ROBOTRON), is demonstrated by using an interactive graphical screen. This program system provides the opportunity to analyze and synthesize mechanisms up to 16 links. The application is demonstrated by designing a front-loader-system for a truck. Real-time animation of seven-bar linkages and coupler curves, including velocity and acceleration, are shown. 相似文献
4.
The identification of targets in visual search arrays may be improved by suppressing competing information from the surrounding distractor items. The present study provided evidence that this hypothetical filtering process has a neural correlate, the N2pc component of the event-related potential waveform. The N2pc was observed when a target item was surrounded by competing distractor items but was absent when the array could be rejected as a nontarget on the basis of simple feature information. In addition, the N2pc was eliminated when filtering was discouraged by removing the distractor items, making the distractors relevant, or making all items within an array identical. Combined with previous topographic analyses, these results suggest that attentional filtering occurs in occipital cortex under the control of feedback from higher cortical regions after a preliminary feature-based analysis of the stimulus array. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
5.
High-performance surface-micromachined inchworm actuator 总被引:1,自引:0,他引:1
de Boer M.P. Luck D.L. Ashurst W.R. Maboudian R. Corwin A.D. Walraven J.A. Redmond J.M. 《Journal of microelectromechanical systems》2004,13(1):63-74
This work demonstrates a polycrystalline silicon surface-micromachined inchworm actuator that exhibits high-performance characteristics such as large force (/spl plusmn/0.5 millinewtons), large velocity range (0 to /spl plusmn/4.4 mm/sec), large displacement range (/spl plusmn/100 microns), small step size (/spl plusmn/10, /spl plusmn/40 or /spl plusmn/100 nanometers), low power consumption (nanojoules per cycle), continuous bidirectional operation and relatively small area (600 /spl times/ 200/spl mu/m/sup 2/). An in situ load spring calibrated on a logarithmic scale from micronewtons to millinewtons, optical microscopy and Michelson interferometry are used to characterize its performance. The actuator consists of a force-amplifying plate that spans two voltage-controlled clamps, and walking is achieved by appropriately sequencing signals to these three components. In the clamps, normal force is borne by equipotential rubbing counterfaces, enabling friction to be measured against load. Using different monolayer coatings, we show that the static coefficient of friction can be changed from 0.14 to 1.04, and that it is load-independent over a broad range. We further find that the static coefficient of friction does not accurately predict the force generated by the actuator and attribute this to nanometer-scale presliding tangential deflections. 相似文献
6.
Lipase-Catalyzed Interesterification of Triglycerides in a Solvent-Free Process I: Analytics and Kinetics of the Interesterification The interesterification of lipids catalyzed by immobilized lipases is an interesting substitute to the chemical interesterification. Studies using an industrial manufactured biocatalyst in a solvent-free process were performed. The kinetics of the interesterification were examined as a function of temperature and water content of the reaction system. The reaction rate of the interesterification can be described with reversible first order reaction kinetics. A part of the triglycerides of the substrate is hydrolyzed by a lipasecatalyzed lipolysis. Hydrolysis products are 1,2(2,3) and 1,3-diglycerides. This shows that an unspecific interesterification is catalyzed if the residence time is extended. 相似文献
7.
GV Naccarelli DL Wolbrette JT Dell'Orfano HM Patel JC Luck 《Canadian Metallurgical Quarterly》1998,9(8):864-891
Multiple trials using antiarrhythmic drugs, pharmacologic therapy, and implantable cardioverter defibrillators have been performed in an attempt to improve survival in patients: (1) postmyocardial infarction; (2) with congestive heart failure, with and without nonsustained ventricular tachycardia; and (3) with sustained ventricular tachycardia and those who have survived an out-of-hospital cardiac arrest. This article reviews some of the key findings and limitations of completed and ongoing trials. We also make recommendations for the current treatment of such patients based on the results of these trials. 相似文献
8.
9.
Conclusion At the National Enterprise Carl Zeiss Jena (East Germany) an improved method of continuous sequential induction hardening of feed screws with highly accurate length was worked out.The method was introduced into production jointly with the firm AEG-Elotherm (West Germany), which designed the corresponding equipment and apparatus.By continuous measurement of the length during continuous sequential surface hardening and immediate computer processing of the results of measurements it is possible by manual control of the speed of the inductor to maintain the elongation of the screw within previously specified narrow limits corresponding to the negative correction of the pitch of the thread envisaged in its cutting.Experience accumulated in recent years shows that the required absolute linear dimension of screws can be maintained with an accuracy of ±0.17 mm, regardless of the length of the screw (within the limits 0.5–4.0 m).Further improvement of the process will be effected chiefly by automating its control which at present is carried out manually.National Enterprise Carl Zeiss Jena (East Germany). AEG-Elotherm, Remscheid. Translated from Metallovedenie i Termicheskaya Obrabotka Metallov, No. 12, pp. 4–8, December, 1984. 相似文献
10.
Natalia Criado Elizabeth Black Michael Luck 《Autonomous Agents and Multi-Agent Systems》2016,30(4):640-680
Norms can be used in multi-agent systems for defining patterns of behaviour in terms of permissions, prohibitions and obligations that are addressed to agents playing a specific role. Agents may play different roles during their execution and they may even play different roles simultaneously. As a consequence, agents may be affected by inconsistent norms; e.g., an agent may be simultaneously obliged and forbidden to reach a given state of affairs. Dealing with this type of inconsistency is one of the main challenges of normative reasoning. Existing approaches tackle this problem by using a static and predefined order that determines which norm should prevail in the case where two norms are inconsistent. One main drawback of these proposals is that they allow only pairwise comparison of norms; it is not clear how agents may use the predefined order to select a subset of norms to abide by from a set of norms containing multiple inconsistencies. Furthermore, in dynamic and non-deterministic environments it can be difficult or even impossible to specify an order that resolves inconsistencies satisfactorily in all potential situations. In response to these two problems, we propose a mechanism with which an agent can dynamically compute a preference order over subsets of its competing norms by considering the coherence of its cognitive and normative elements. Our approach allows flexible resolution of normative inconsistencies, tailored to the current circumstances of the agent. Moreover, our solution can be used to determine norm prevalence among a set of norms containing multiple inconsistencies. 相似文献