Preparation of gold nanoparticles (GNP) aqueous suspensions by a new method involving Tiron

A new method is proposed to produce gold nanoparticles (GNP) by in situ reduction of a gold salt dissolved in water. The reducing agent used is Tiron instead of the citrate anion most often mentioned in literature. The influence of various parameters has been investigated, such as the content of Tiron with respect to that of the precursor of gold HAuCl₄, or the initial pH of the solution after mixing of reactants. It is shown that Tiron also exerts a positive influence as a dispersant, which impedes agglomeration of gold nanoparticles. The typical average size of GNP synthesized in the present work is close to 7 nm.     

Time‐averaged spatial profile of Xe excitation efficiency in PDPs

Abstract— The Xe excitation efficiency for various Xe content was analyzed by monitoring the panel luminance and IR emission intensity. It was found that dependences of the Xe excitation efficiency and luminous efficacy on the sustain voltage show almost the same tendency. A decrease for increasing sustaining voltage was found in a low‐Xe‐content panel and an increase was found in a high‐Xe‐content panel. A reduction in the effective electron temperature and a reduction in plasma saturation contribute to the efficacy improvement. The time‐averaged spatial profile of the Xe excitation efficiency in PDPs was investigated by measuring the distribution of IR and blue‐phosphor emissions. The results show that the Xe excitation efficiency is similar in the cathode and anode regions even though the spatial and time development of the discharge in these regions is very different. An extended theory that takes into account not only the radiative transition process but also the collisional de‐excitation process from Xe** to Xe* is proposed for investigating the pressure dependence of the Xe excitation efficiency. By using the proposed theory, it was found that Xe excitation efficiency increases, attains a maximum value at 30% Xe, then decreases as the Xe content is increased, when the rate coefficient of the collisional de‐excitation process is less than 1.0 × 10^?10 cm³/sec.     

Staphylococcus aureus Infection-Related Glomerulonephritis with Dominant IgA Deposition

Since 1995, when we reported the case of a patient with glomerulonephritis with IgA deposition that occurred after a methicillin-resistant Staphylococcus aureus (MRSA) infection, many reports of MRSA infection-associated glomerulonephritis have accumulated. This disease is being systematized as Staphylococcus infection-associated glomerulonephritis (SAGN) in light of the apparent cause of infection, and as immunoglobulin A-dominant deposition infection-related glomerulonephritis (IgA-IRGN) in light of its histopathology. This glomerulonephritis usually presents as rapidly progressive glomerulonephritis or acute kidney injury with various degrees of proteinuria and microscopic hematuria along with an ongoing infection. Its renal pathology has shown several types of mesangial and/or endocapillary proliferative glomerulonephritis with various degrees of crescent formation and tubulointerstitial nephritis. IgA, IgG, and C₃ staining in the mesangium and along the glomerular capillary walls have been observed on immunofluorescence examinations. A marked activation of T cells, an increase in specific variable regions of the T-cell receptor β-chain-positive cells, hypercytokinemia, and increased polyclonal immune complexes have also been observed in this glomerulonephritis. In the development of this disease, staphylococcal enterotoxin may be involved as a superantigen, but further investigations are needed to clarify the mechanisms underlying this disease. Here, we review 336 cases of IgA-IRGN and 218 cases of SAGN.     

Association of Ligamentum Flavum Hypertrophy with Adolescent Idiopathic Scoliosis Progression—Comparative Microarray Gene Expression Analysis

The role of the ligamentum flavum (LF) in the pathogenesis of adolescent idiopathic scoliosis (AIS) is not well understood. Using magnetic resonance imaging (MRI), we investigated the degrees of LF hypertrophy in 18 patients without scoliosis and on the convex and concave sides of the apex of the curvature in 22 patients with AIS. Next, gene expression was compared among neutral vertebral LF and LF on the convex and concave sides of the apex of the curvature in patients with AIS. Histological and microarray analyses of the LF were compared among neutral vertebrae (control) and the LF on the apex of the curvatures. The mean area of LF in the without scoliosis, apical concave, and convex with scoliosis groups was 10.5, 13.5, and 20.3 mm², respectively. There were significant differences among the three groups (p < 0.05). Histological analysis showed that the ratio of fibers (Collagen/Elastic) was significantly increased on the convex side compared to the concave side (p < 0.05). Microarray analysis showed that ERC2 and MAFB showed significantly increased gene expression on the convex side compared with those of the concave side and the neutral vertebral LF cells. These genes were significantly associated with increased expression of collagen by LF cells (p < 0.05). LF hypertrophy was identified in scoliosis patients, and the convex side was significantly more hypertrophic than that of the concave side. ERC2 and MAFB genes were associated with LF hypertrophy in patients with AIS. These phenomena are likely to be associated with the progression of scoliosis.     

Direct Reprogramming and Induction of Human Dermal Fibroblasts to Differentiate into iPS-Derived Nucleus Pulposus-like Cells in 3D Culture

Intervertebral disc (IVD) diseases are common spinal disorders that cause neck or back pain in the presence or absence of an underlying neurological disorder. IVD diseases develop on the basis of degeneration, and there are no established treatments for degeneration. IVD diseases may therefore represent a candidate for the application of regenerative medicine, potentially employing normal human dermal fibroblasts (NHDFs) induced to differentiate into nucleus pulposus (NP) cells. Here, we used a three-dimensional culture system to demonstrate that ectopic expression of MYC, KLF4, NOTO, SOX5, SOX6, and SOX9 in NHDFs generated NP-like cells, detected using Safranin-O staining. Quantitative PCR, microarray analysis, and fluorescence-activated cell sorting revealed that the induced NP cells exhibited a fully differentiated phenotype. These findings may significantly contribute to the development of effective strategies for treating IVD diseases.     

Quantification of dance movement by simultaneous measurement of body motion and biophysical information

The purpose of this research is a quantitative analysis of movement patterns of dance,which cannot be analyzed with a motion capture system alone,using simultaneous measurement of body motion and biophysical information.In this research,two kinds of same leg movement are captured by simultaneous measurement;one is a leg movement with given strength,the other is a leg movement without strength on condition of basic experiment using optical motion capture and electromyography (EMG) equipment in order to quantitatively analyze characteristics of leg movement.Also,we measured the motion of the traditional Japanese dance using the constructed system.We can visualize leg movement of Japanese dance by displaying a 3D CG character animation with motion data and EMG data.In addition,we expect that our research will help dancers and researchers on dance through giving new information on dance movement which cannot be analyzed with only motion capture.     

Automated single nucleotide polymorphism typing using bead array in capillary tube

A low-cost and simple on-site technique for genotyping single nucleotide polymorphisms (SNPs) was developed. The technique is based on allele-specific primer PCR and the recently developed bead arrays in a single tip technique. The performance of the method was verified by genotyping four SNPs that correlate with cardiovascular diseases.     

Two Step Timing Synchronization Scheme for OFDM Signal in General Purpose Processor Based Software Defined Radio Receiver

Software defined radio (SDR) is a technology that allows a single terminal to support various kinds of wireless systems by changing its software to reconfigure itself. A general purpose processor (GPP) based SDR receiver platform named Sora has been recently developed by Microsoft. In the GPP based SDR receiver, timing synchronization of an OFDM signal consumes a significant amount of computational resources in the GPP. In this paper, a timing synchronization scheme which uses delayed correlation and matched filtering for the GPP based SDR platform is evaluated. The two stage timing synchronization scheme reduces the computational complexity by limiting the timing range of matched filtering. The proposed scheme reduces the amount of data transmission between the memory and the GPP of the SDR platform. It is shown through an experiment that the proposed scheme reduces the number of cycles for timing synchronization by up to 30 %.     

Signal Detection Performance of Overlapped FFT Scheme with Additional Frames Consisting of Non-continuous Samples in Indoor Environment

Overlapped FFT has been proposed as a signal detection scheme in dynamic spectrum access to reduce the variance of the noise and improve the detection probability. However, the improvement of the detection probability in the conventional overlapped FFT is bounded with the upper limit of the overlap ratio. This paper proposes a new overlapped FFT scheme using additional frames. In the proposed scheme, in addition to the original FFT frames, new frames that consist of multiple subframes with non-continuous samples are constructed and included. It can realize the increase of the number of the FFT frames and the improvement of the detection probability compared with the conventional scheme. Numerical results through computer simulation show that the proposed scheme improves the detection probability by up to 0.07. On indoor channel models the proposed scheme also improves the detection probability. In addition, it is clarified that as the delay spread increases the detection probability reduces due to the correlation between the frames.     

Are hybrid sit–stand postures a good compromise between sitting and standing?

Potential alternatives for conventional sitting and standing postures are hybrid sit-stand postures (i.e. perching). The purposes of this study were (i) to identify where lumbopelvic and pelvic angles deviate from sitting and standing and (ii) to use these breakpoints to define three distinct postural phases: sitting, perching, and standing, in order to examine differences in muscle activations and ground reaction forces between phases. Twenty-four participants completed 19 1-min static trials, from sitting (90°) to standing (180°), sequentially in 5°trunk–thigh angle increments. The perching phase was determined to be 145–175° for males and 160–175° for females. For both sexes, knee extensor activity was lower in standing compared to perching or sitting (p < .01). Anterior–posterior forces were the highest in perching (p < .001), requiring ～15% of body-weight. Chair designs aimed at reducing the lower limb demands within 115–170° trunk–thigh angle may improve the feasibility of sustaining the perched posture.

Practitioner summary: Individuals who develop low back pain in sitting or standing may benefit from hybrid sit-stand postures (perching), yet kinematic and kinetic changes associated with these postures have not been investigated. Perching can improve lumbar posture at a cost of increased lower limb demands, suggesting potential avenues for chair design improvement.

Abbreviations: A/P: anterior-posterior; M/L: medial-lateral; LBP: low back pain; EMG: electromyography; TES: thoracic erector spinae; LES: lumbar erector spinae; VMO: vastus medialis obliquus; MVC: maximum voluntary contraction; ASIS: anterior superior iliac spine; PSIS: posterior superior iliac spine; BW: body weight; RMSE: root mean square error; SD: standard deviation; ROM: range of motion