Determinants of genital human papillomavirus infection among cytologically normal women attending the University of New Mexico student health center

OBJECTIVES: To confirm the risk factors for genital human papillomavirus (HPV) infection. GOAL OF THIS STUDY: To investigate risk factors for HPV detection apart from the correlated risk factors for cervical neoplasia. STUDY DESIGN: Cervical human papillomavirus (HPV) DNA was assessed in 357 cytologically normal women attending the University of New Mexico student health center. Cervical swab samples were obtained for HPV DNA detection and typing using a PCR-based DNA amplification system. Possible determinants of cervical HPV were examined including age, ethnicity, history of sexually transmitted disease, oral contraceptive use, smoking, age at first intercourse, lifetime number of sex partners, marital status, and history of pregnancy. RESULTS: A 44.3% overall prevalence of cervical HPV was observed. On univariate analysis, factors associated with increasing HPV prevalence included higher lifetime number of sex partners and single marital status. After adjustment for potential confounding variables, we found that HPV prevalence increased with higher lifetime number of sexual partners. CONCLUSION: These findings, along with those from the companion reports in this issue of the journal, support the sexual route of transmission of the virus.     

Prediction of Experimental Methanol Decomposition Rates on Platinum from First Principles

A microkinetic model for methanol decomposition on platinum is presented. The model incorporates competitive decomposition pathways, beginning with both O–H and C–H bond scission in methanol, and uses results from density functional theory (DFT) calculations [Greeley and Mavrikakis, J. Am. Chem. Soc. 124 (2002) 7193, Greeley and Mavrikakis, J. Am. Chem. Soc. 126 (2004) 3910]. Results from reaction kinetics experiments show that the rate of H₂ production increases with increasing temperature and methanol concentration in the feed and is only nominally affected by the presence of CO or H₂ with methanol. The model, based on the values of binding energies, pre-exponential factors and activation energy barriers derived from first principles calculations, accurately predicts experimental reaction rates and orders. The model also gives insight into the most favorable reaction pathway, the rate-limiting step, the apparent activation energy, coverages, and the effects of pressure. It is found that the pathway beginning with the C–H bond scission (CH₃OH→H₂COH→HCOH→CO) is dominant compared with the path beginning with O–H bond scission. The cleavage of the first C–H bond in methanol is the rate-controlling step. The surface is highly poisoned by CO, whereas COH appears to be a spectator species.     

Scanning tunnelling microscopy of tobacco mosaic virus on evaporated and sputter-coated palladium/gold substrates

We present scanning tunnelling microscope (STM) images of untreated tobacco mosaic virus (TMV) deposited on thermally evaporated and on sputter-coated palladium/gold 40:60 (Pd/Au) substrates, and imaged under ordinary atmospheric conditions. The TMV imaged on both evaporated and sputter-coated substrates was consistently several times wider than the known diameter of the virus. TMV on evaporated Pd/Au became overcoated with Pd/Au material during sample preparation and appeared elevated in STM images, whereas TMV on sputter-coated Pd/Au appeared as depressions. When naked TMV were intentionally overcoated with Pd/Au, the STM images were found to be similar to those for TMV on evaporated Pd/Au.     

Formic acid decomposition on Au catalysts: DFT,microkinetic modeling,and reaction kinetics experiments

A combined theoretical and experimental approach is presented that uses a comprehensive mean‐field microkinetic model, reaction kinetics experiments, and scanning transmission electron microscopy imaging to unravel the reaction mechanism and provide insights into the nature of active sites for formic acid (HCOOH) decomposition on Au/SiC catalysts. All input parameters for the microkinetic model are derived from periodic, self‐consistent, generalized gradient approximation (GGA‐PW91) density functional theory calculations on the Au(111), Au(100), and Au(211) surfaces and are subsequently adjusted to describe the experimental HCOOH decomposition rate and selectivity data. It is shown that the HCOOH decomposition follows the formate (HCOO) mediated path, with 100% selectivity toward the dehydrogenation products (CO₂ + H₂) under all reaction conditions. An analysis of the kinetic parameters suggests that an Au surface in which the coordination number of surface Au atoms is ≤4 may provide a better model for the active site of HCOOH decomposition on these specific supported Au catalysts. © 2014 American Institute of Chemical Engineers AIChE J, 60: 1303–1319, 2014     

Transesterification of rapeseed oil for the production of biodiesel using homogeneous and heterogeneous catalysis

In the present work, the transesterification reaction of rapeseed oil with methanol, in the presence of alkaline catalysts, either homogeneous (NaOH) or heterogeneous (Mg MCM-41, Mg–Al Hydrotalcite, and K+ impregnated zirconia), using low frequency ultrasonication (24 kHz) and mechanical stirring (600 rpm) for the production of biodiesel fuel was studied. Selection of heterogeneous catalysts was based on a combination of their porosity and surface basicity. Their characterization was carried out using X-ray diffraction (XRD), Nitrogen adsorption–desorption porosimetry and scanning electron microscopy (SEM) with energy dispersive spectroscopy (EDS). The activities of the catalysts were related to their basic strength. Mg–Al hydrotalcite showed particularly the highest activity with conversion reaching 97%). The activity of ZrO₂ in the transesterification reaction increased as the catalyst was doped with more potassium cations, becoming thus more basic. Use of ultrasonication significantly accelerated the transesterification reaction compared to the use of mechanical stirring (5 h vs. 24 h).Given the differences in experimental design, it can be concluded that the homogeneous catalyst accelerated significantly the transesterification reaction, as compared to all heterogeneous catalysts, using both mechanical stirring (15 min vs. 24 h) and ultrasonication (10 min vs. 5 h). However, the use of homogeneous base catalysts requires neutralization and separation from the reaction mixture leading to a series of environmental problems related to the use of high amounts of solvents and energy. Heterogeneous solid base catalysts can be easily separated from the reaction mixture by simple filtration, they are easily regenerated and bear a less corrosive nature, leading to safer, cheaper and more environment-friendly operations.     

Design rule development for microwave flip-chip applications

This paper presents a novel experimental approach for the analysis of factors to be considered when designing a flip-chip package. It includes the design of an experiment and statistical analysis of the outputs and uses both test-structure measurements and full-wave simulation techniques in the 1-35-GHz frequency range. The most significant factors are found to be, from the most to least important, the length of the area where the device and substrate overlap (referred to as conductor overlap), the bump diameter, and the width of the coplanar-waveguide transmission-line launch. These results are valid for conductor overlaps between 300-500 μm. For a lower value (120 μm), the significance level of the overlap decreases and the bump height also becomes significant. Test-structure measurements in the 120-200-μm overlap range validate this result and demonstrate the decrease in the significance level. The substrate thickness in the 10-25-mil interval is found to be statistically insignificant, therefore, it can be eliminated from further analysis. This approach provides a foundation for development of a set of design rules for RF and microwave flip-chip similar to RF integrated-circuit design rules     

Low-loss LTCC cavity filters using system-on-package technology at 60 GHz

In this paper, three-dimensional (3-D) integrated cavity resonators and filters consisting of via walls are demonstrated as a system-on-package compact solution for RF front-end modules at 60 GHz using low-temperature cofired ceramic (LTCC) technology. Slot excitation with a /spl lambda/g/4 open stub has been applied and evaluated in terms of experimental performance and fabrication accuracy and simplicity. The strongly coupled cavity resonator provides an insertion loss <0.84 dB, a return loss >20.6 dB over the passband (/spl sim/0.89 GHz), and a 3-dB bandwidth of approximately 1.5% (/spl sim/0.89 GHz), as well as a simple fabrication of the feeding structure (since it does not require to drill vias to implement the feeding structure). The design has been utilized to develop a 3-D low-loss three-pole bandpass filter for 60-GHz wireless local area network narrow-band (/spl sim/1 GHz) applications. This is the first demonstration entirely authenticated by measurement data for 60-GHz 3-D LTCC cavity filters. This filter exhibits an insertion loss of 2.14 dB at the center frequency of 58.7 GHz, a rejection >16.4 dB over the passband, and a 3-dB bandwidth approximately 1.38% (/spl sim/0.9 GHz).     

PML absorbing boundary conditions for the characterization of openmicrowave circuit components using multiresolution time-domaintechniques (MRTD)

The multiresolution time domain technique (MRTD) is applied to the modeling of open microwave circuit problems. Open boundaries are simulated by the use of a novel formulation of the perfect matching layer (PML) absorber. The PML is modeled both in split and nonsplit forms and can be brought right on the surface of the planar components. The applicability of the MRTD technique to complex geometries with high efficiency and accuracy in computing the fields at discontinuities is demonstrated through extensive comparisons to conventional finite difference time domain (FDTD). In addition, the numerical reflectivity of the PML absorber is investigated for a variety of cell sizes, some of which are very close to the Nyquist limit (λ/2)     

A novel wideband and circularly polarized cross‐dipole antenna

In this paper, we present a novel wideband circularly polarized (CP) composite, called cavity‐backed crossed dipole antenna for 2.45 GHz industrial, scientific, and medical (ISM) band wireless communication. To excite the CP radiation effectively, a curved‐delay line providing an orthogonal phase difference among the cross‐dipole elements is attached at corners of the sequentially rotated elements. By choosing a proper radius of the curved‐delay line, a wide input impedance of the antenna can be realized. Unlike conventional cross‐dipole antennas, the proposed cross‐dipole antenna is designed with an open stub added to the radiating arms of the dipole so that both impedance and axial ratio bandwidths are enhanced. The antenna is center‐fed by a 50‐Ω coaxial cable and is placed above a cavity‐backed reflector to obtain a directional CP radiation pattern. With the advantage of being center‐fed, a symmetric CP radiation pattern can be achieved across the entire operating bandwidth. To further improve the directivity and the radiation pattern, a rectangular cavity‐backed reflector is used. Simulated and measured results confirm that the proposed antenna has good CP characteristics. The proposed antenna obtains a broad 3‐dB axial ratio bandwidth of 49% (1.20 GHz, 1.96–3.16 GHz) and an impedance bandwidth of 67.7% (1.66 GHz, 1.69–3.35 GHz) for reflection coefficient (S₁₁) ≦ −10 dB. It also yields an average CP gain of 9.2 dBic across the operating bandwidth and a peak CP gain of 10 dBic. Copyright © 2016 John Wiley & Sons, Ltd.     

Ligand-Specific Nano-Contrast Agents Promote Enhanced Breast Cancer CT Detection at 0.5 mg Au

For many cancer types, being undetectable from early symptoms or blood tests, or often detected at late stages, medical imaging emerges as the most efficient tool for cancer screening. MRI, ultrasound, X-rays (mammography), and X-ray CT (CT) are currently used in hospitals with variable costs. Diagnostic materials that can detect breast tumors through molecular recognition and amplify the signal at the targeting site in combination with state-of-the-art CT techniques, such as dual-energy CT, could lead to a more precise detection and assist significantly in image-guided intervention. Herein, we have developed a ligand-specific X-ray contrast agent that recognizes α5β1 integrins overexpressed in MDA-MB-231 breast cancer cells for detection of triple (−) cancer, which proliferates very aggressively. In vitro studies show binding and internalization of our nanoprobes within those cells, towards uncoated nanoparticles (NPs) and saline. In vivo studies show high retention of ~3 nm ligand-PEG-S-AuNPs in breast tumors in mice (up to 21 days) and pronounced CT detection, with statistical significance from saline and iohexol, though only 0.5 mg of metal were utilized. In addition, accumulation of ligand-specific NPs is shown in tumors with minimal presence in other organs, relative to controls. The prolonged, low-metal, NP-enhanced spectral-CT detection of triple (−) breast cancer could lead to breakthrough advances in X-ray cancer diagnostics, nanotechnology, and medicine.