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排序方式: 共有95条查询结果,搜索用时 62 毫秒
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Mariia Belinskaia Tomas Zurawski Seshu Kumar Kaza Caren Antoniazzi J. Oliver Dolly Gary W. Lawrence 《International journal of molecular sciences》2022,23(2)
Nerve growth factor (NGF) is known to intensify pain in various ways, so perturbing pertinent effects without negating its essential influences on neuronal functions could help the search for much-needed analgesics. Towards this goal, cultured neurons from neonatal rat trigeminal ganglia—a locus for craniofacial sensory nerves—were used to examine how NGF affects the Ca2+-dependent release of a pain mediator, calcitonin gene-related peptide (CGRP), that is triggered by activating a key signal transducer, transient receptor potential vanilloid 1 (TRPV1) with capsaicin (CAP). Measurements utilised neurons fed with or deprived of NGF for 2 days. Acute re-introduction of NGF induced Ca2+-dependent CGRP exocytosis that was inhibited by botulinum neurotoxin type A (BoNT/A) or a chimera of/E and/A (/EA), which truncated SNAP-25 (synaptosomal-associated protein with Mr = 25 k) at distinct sites. NGF additionally caused a Ca2+-independent enhancement of the neuropeptide release evoked by low concentrations (<100 nM) of CAP, but only marginally increased the peak response to ≥100 nM. Notably, BoNT/A inhibited CGRP exocytosis evoked by low but not high CAP concentrations, whereas/EA effectively reduced responses up to 1 µM CAP and inhibited to a greater extent its enhancement by NGF. In addition to establishing that sensitisation of sensory neurons to CAP by NGF is dependent on SNARE-mediated membrane fusion, insights were gleaned into the differential ability of two regions in the C-terminus of SNAP-25 (181–197 and 198–206) to support CAP-evoked Ca2+-dependent exocytosis at different intensities of stimulation. 相似文献
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Viktor O. Iaroshenko Dmytro Ostrovskyi Mariia Miliutina Aneela Maalik Alexander Villinger Andrei Tolmachev Dmitriy M. Volochnyuk Peter Langer 《Advanced Synthesis \u0026amp; Catalysis》2012,354(13):2495-2503
A new strategy for the synthesis of polycyclic imidazole‐containing N‐heterocycles, based on the two general synthetic ways, namely the Pd(II)‐catalyzed intramolecular arylation via CH/C Hal and CH/CH coupling reactions, was developed. The method proposed here enables the synthesis of many fused N‐heterocycles containing purine, 1‐deazapurines and benzimidazole structural units. 相似文献
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AbstractDigitisation of historical costumes is an actual multidisciplinary area of research that uses science-based methods of reconstruction in virtual reality. The main aim of this study was to create a method for generating numerical replicas of skirts of the late 1850?s and the1860s. We applied two-dimensional and three-dimensional software to parameterise all the elements of the skirts and recreate them layer by layer. Computer modelling allowed us to gain insight into the interrelation between the parameters of cage crinolines, skirt construction and the behaviour of textile materials on the crinoline’s surface. A replica of a historical costume was generated and the similarity between the historical prototype and its replica was proved. The application of computer graphics tools for the reconstruction of the visible and invisible elements of historical costumes can advance their scientific study. The reconstructions can be an effective instrument for teaching, enriching museum collections and producing online presentations. 相似文献
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Mariia Antipchik Apollinariia Dzhuzha Vasilii Sirotov Tatiana Tennikova Evgenia Korzhikova-Vlakh 《应用聚合物科学杂志》2021,138(12):50070
In present work, the development of macroporous monolithic layers bearing the artificial recognition sites toward L-phenylalanine has been carried out. The set of macroporous poly(2-aminoethyl methacrylate-co-2-hydroxyethyl methacrylate-co-ethylene glycol dimethacrylate) materials with average pore size ranged in 340–1200 nm was synthesized. The applicability of Hildebrand's and Hansen's theories for the prediction of polymer compatibility with porogenic solvents was evaluated. The dependences of average pore size on theoretically calculated parameters were plotted. The linear trend detected for Hansen's theory has indicated the high suitability of this approach to select appropriate porogens. The synthesized monolithic MIP layers were tested toward the ability to rebind phenylalanine-derivative in microarray format. The influence of such factors as average pore size of the material, the concentration of template molecule in polymerization mixture, interaction time of analyte with its imprinted sites on binding efficiency were studied. The developed materials demonstrated good analyte rebinding from buffer solution with recognition factors 2.5–3.4 depending on the MIP sample. The comparable rebinding efficiency was also detected when the analysis was carried using complex biological media. The selectivity of phenylalanine binding from the equimolar mixture of structural analogues was 81.9% for free amino acid and 91.2% for labeled one. 相似文献
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B. Ya. Ryabko A. E. Gus’kov I. V. Selivanova 《Problems of Information Transmission》2017,53(3):294-304
We consider a method for automatic (i.e., unmanned) text classification based on methods of universal source coding (or “data compression”). We show that under certain restrictions the proposed method is consistent, i.e., the classification error tends to zero with increasing text lengths. As an example of practical use of the method we consider the classification problem for scientific texts (research papers, books, etc.). The proposed method is experimentally shown to be highly efficient. 相似文献